ISSN:
1435-1463
Keywords:
Phosphoinositide hydrolysis
;
hippocampus
;
serotonin
;
para-chlorophenylalanine
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Serotonin (5-HT) caused a dose-dependent accumulation of inositol monophosphate (IP-1) in rat hippocampal slices (maximal effect +172%, EC50=630 nM) in the presence of LiCl. The 5-HT response was blocked potently by a non-selective 5-HT receptor antagonist metergoline and a 5-HT1C/ 5-HT2 receptor antagonist ritanserin. The 5-HT2 receptor antagonist ketanserin and spiperone were, however, less potent at inhibiting the 5-HT response, m-Chlorophenylpiperazine (mCPP), a 5-HT1C receptor agonist but a 5-HT2 antagonist stimulated directly PI turnover, yet mCPP inhibited 5-HT-stimulated PI response. These findings indicate that both 5HT1C and 5-HT2 receptors are involved with a complicated interaction of each receptor in 5-HT-stimulated PI hydrolysis in rat hippocampus. 10-Day treatment with para-chlorophenylalanine (PCPA), a 5-HT synthesis inhibitor, resulted in a significant increase (maximal effect +225%, EC50=580 nM) in 5-HT-stimulated IP-1 accumulation with no substantial change in EC50 value, which suggest that subchronic treatment with PCPA enhances the 5-HT-mediated PI hydrolysis in rat hippocampus.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01245119
Permalink