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  • 1
    Electronic Resource
    Electronic Resource
    Effects of glucose, leucine and adenosine on insulin release, ^4^5Ca^2^+ net uptake, NADH/NAD ratios and oxygen consumption of islets isolated from fed and starved mice
    Amsterdam : Elsevier
    Molecular and Cellular Endocrinology 30 (1983), S. 51-62 
    Details
    ISSN: 0303-7207
    Keywords: ^4^5Ca^2^+ net uptake ; insulin release ; mouse pancreatic islets ; nutrient secretagogues ; oxidative metabolism ; starvation
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0303-7207(83)90200-9
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  • 2
    Electronic Resource
    Electronic Resource
    The renal subcapsular site offers better growth conditions for transplanted mouse pancreatic islet cells than the liver or spleen (1986)
    Springer
    Diabetologia 29 (1986), S. 670-672 
    Details
    ISSN: 1432-0428
    Keywords: Mouse panreatic islets ; islet transplantation ; implantation sites ; autoradiography ; labelling index ; islet cell replication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In order to investigate the importance of the transplantation site for the replication of grafted islet cells, we implanted syngeneic mouse pancreatic islets intrasplenically, intraportally and subcapsularly in the kidney. Fourteen days later the alloxan-diabetic mice were killed after an injection of tritiated thymidine, and the graft-bearing organs fixed and autoradiography. The highest labelling indices were recorded for subcapsularly grafted islets, followed by intraportal and intrasplenic islets in that order. In separate experiments some islet-containing kidney sections were immune stained for insulin before the autoradiographic process. The labelling index of the insulin-positive cells was as high as in the entire islet cell population of the sections from the same mice stained with haematoxylin only. This indicates that the B cells of the islets replicate as often as the other islet cell types. The present data also suggest that the renal subcapsular space offers better growth conditions for transplanted islet cells than the liver or spleen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00869269
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Rapid deposition of amyloid in human islets transplanted into nude mice (1995)
    Springer
    Diabetologia 38 (1995), S. 543-549 
    Details
    ISSN: 1432-0428
    Keywords: Islet amyloid polypeptide ; human pancreatic islets ; islet transplantation ; immune histochemistry ; hyperglycaemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Human islets of Langerhans were transplanted to the subcapsular space of the kidneys of nude mice which were either normoglycaemic or made diabetic with alloxan. After 2 weeks, the transplants were processed for light and electron microscopical analyses. In all transplants, islet amyloid polypeptide (IAPP)-positive cells were found with highest frequency in normoglycaemic animals. IAPP-positive amyloid was seen in 16 out of 22 transplants (73%), either by polarisation microscopy after Congo red staining or by immune electron microscopy. At variance with previous findings of amyloid deposits exclusively in the extracellular space of islets of non-insulin-dependent diabetic patients, the grafted islets contained intracellular amyloid deposits as well. There was no clear difference in occurrence of amyloid between diabetic and non-diabetic animals. The present study indicates that human islets transplanted into nude mice very soon present IAPP-positive amyloid deposits. This technique may provide a valuable model for studies of the pathogenesis of islet amyloid and its impact on islet cell function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00400722
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Long-term effects of exposure of pancreatic islets to nicotinamide in vitro on DNA synthesis, metabolism and B-cell function (1986)
    Springer
    Diabetologia 29 (1986), S. 199-202 
    Details
    ISSN: 1432-0428
    Keywords: Autoradiography ; insulin release ; DNA synthesis ; nicotinamide ; pancreatic islets ; poly(ADP-ribose) ; synthetase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Using3H-thymidine labeling techniques, we found that rates of DNA synthesis in islet cells doubled when mouse pancreatic islets were cultured for 1 week with 10 mmol/1 nicotinamide, a potent poly(ADP-ribose) synthetase inhibitor. Culture with nicotinamide partially inhibited glucose-stimulated insulin release, whereas the islet insulin content and rate of (pro)insulin biosynthesis remained unchanged. Long-term exposure to nicotinamide decreased glucose oxidation and ATP content in the islets. The findings support the view that poly(ADP-ribose)synthetase inhibitors stimulate islet cell replication, but may be accompanied by significant inhibitory effects on islet cell function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02427093
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  • 5
    Electronic Resource
    Electronic Resource
    Failure of successful intrasplenic transplantation of islets from lean mice to cure obese-hyperglycaemic mice, despite islet growth (1981)
    Springer
    Diabetologia 20 (1981), S. 237-241 
    Details
    ISSN: 1432-0428
    Keywords: Obese-hyperglycaemic mice ; isolated pancreatic islets ; islet transplantation ; serum glucose ; islet volume ; autoradiography ; islet cell replication ; immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Implantation of allogeneic pancreatic islets encapsulated in Millipore diffusion chambers has been reported to normalize the obese-hyperglycaemic syndrome in mice. In the present study, both young and adult ob/ob mice remained hyperglycaemic and gained weight after intrasplenic implantation of 500 isogeneic islets isolated from lean mice. Such islets normalized the elevated blood-glucose of alloxan-diabetic lean mice. Morphometric analysis of the intrasplenically implanted islets showed that the mean islet volume in the ob/ob mice was five times larger than that of the lean, non-diabetic mice. Immunocytochemical staining of the spleens showed an increased proportion of B-cells in the enlarged, intrasplenic islets in the ob/ob mice. Moreover, autoradiographical examination of these islets demonstrated the presence of several labelled cells. These results suggest that the growth of the implanted “lean” islets is due to extrapancreatic factors which stimulate islet cell replication in the obese-hyperglycaemic mouse.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252634
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Rapid deposition of amyloid in human islets transplanted into nude mice (1995)
    Springer
    Diabetologia 38 (1995), S. 543-549 
    Details
    ISSN: 1432-0428
    Keywords: Key words Islet amyloid polypeptide ; human pancreatic islets ; islet transplantation ; immune histochemistry ; hyperglycaemia.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Human islets of Langerhans were transplanted to the subcapsular space of the kidneys of nude mice which were either normoglycaemic or made diabetic with alloxan. After 2 weeks, the transplants were processed for light and electron microscopical analyses. In all transplants, islet amyloid polypeptide (IAPP)-positive cells were found with highest frequency in normoglycaemic animals. IAPP-positive amyloid was seen in 16 out of 22 transplants (73 %), either by polarisation microscopy after Congo red staining or by immune electron microscopy. At variance with previous findings of amyloid deposits exclusively in the extracellular space of islets of non-insulin-dependent diabetic patients, the grafted islets contained intracellular amyloid deposits as well. There was no clear difference in occurrence of amyloid between diabetic and non-diabetic animals. The present study indicates that human islets transplanted into nude mice very soon present IAPP-positive amyloid deposits. This technique may provide a valuable model for studies of the pathogenesis of islet amyloid and its impact on islet cell function. [Diabetologia (1995) 38: 543–549]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00400722
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Effects of glucose on the ultrastructure and insulin biosynthesis of isolated mouse pancreatic islets maintained in tissue culture (1974)
    Springer
    Diabetologia 10 (1974), S. 743-753 
    Details
    ISSN: 1432-0428
    Keywords: Tissue culture ; pancreatic islets ; degranulation ; endoplasmic reticulum ; insulin content ; insulin release ; insulin degradation ; insulin biosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Isolated mouse pancreatic islets were maintained in tissue culture for up to 12 days in glucose concentrations varying between 3.3 and 28 ml. A satisfactory ultrastructural preservation of the islet cells was found irrespective of the glucose concentration of the culture medium. While the B-cells of islets cultured in the lower glucose concentration showed slight degranulation, there was extensive degranulation and increased amounts of rough-surfaced endoplasmic reticulum after culture in the higher glucose concentration. The immunoreactive insulin content of islets cultured at 28 mM glucose was markedly decreased and the insulin secretion during the culture period was much higher than that of islets cultured at 3.3 mM glucose. The insulin biosynthesis, as reflected in the incorporation of3H-leucine into gel chromatographed extracts of acid-ethanol soluble islet proteins, was studied either during the culture period or at the end of a 6-day culture in short-term incubations lasting for 90 or 180 min. The results consistently showed that the biosynthesis of insulin proceeded at a high rate and remained regulated by glucose throughout the culture period. The continuous addition of newly synthesized and labelled insulin to the small intracellular insulin pool of the high-glucose cultured B-cells produced a very high specific radioactivity of the insulin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01219536
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