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  • 1
    Electronic Resource
    Electronic Resource
    Disturbed sugar metabolism in a fully habituated nonorganogenic callus of Beta vulgaris (L.) (1993)
    Springer
    Plant growth regulation 13 (1993), S. 257-261 
    Details
    ISSN: 1573-5087
    Keywords: Beta vulgaris ; glycolysis ; hexose monophosphate pathway ; sugarbeet ; sugar metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Habituated (H) nonorganogenic sugarbeet callus was found to exhibit a disturbed sugar metabolism. In contrast to cells from normal (N) callus, H cells accumulate glucose and fructose and show an abnormal high fructose/glucose ratio. Moreover, H cells which have decreased wall components, display lower glycolytic enzyme activities (hexose phosphate isomerase and phosphofructokinase) which is compensated by higher activities of the enzymes of the hexose monophosphate pathway (glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase). The disturbed sugar metabolism of the H callus is discussed in relation to a deficiency in H2O2 detoxifying systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024846
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Low activity of amine-oxidases and accumulation of conjugated polyamines in disfavour of organogenic programs in Chrysanthemum leaf disc explants (1998)
    Springer
    Plant cell, tissue and organ culture 55 (1998), S. 85-94 
    Details
    ISSN: 1573-5044
    Keywords: budding ; callus ; polyamine anabolism ; polyamine catabolism ; rooting ; transglutaminase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Foliar discs (8 mm diameter) from expanding leaves of the middle part of vegetative shoots of Chrysanthemum morifolium Ramat raised in vitro were induced to form directly on specific media in vitro either roots or vegetative buds, or callus. The budding programme, on its specific medium, was deviated to callus formation by the addition of 2 mM β-OH-E (β-OH-ethyldrazine, an inhibitor of diamine oxidase). Conversely vegetative buds instead of callus were formed on the callus medium in the presence of 2 mM DFMO (difluoromethylornithine, an inhibitor of ornithine decarboxylase). Callus formation was characterized by high accumulation of free and particularly conjugated polyamines (PA), very low or undetectable activities of diamine- and polyamine oxidases, and transglutaminase. DFMO-deviation of callus initiation in favour of bud formation lowered the accumulation of PA and increased the activity of amine-oxidases. The high catabolism of PA in the organogenic (rooting, budding) programs was questioned as to its role in developmental processes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006153806610
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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