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  • 1
    Electronic Resource
    Electronic Resource
    Cytosolic thymidine kinase activity in cultured human fibroblasts from individuals with galactokinase deficiency (1989)
    Springer
    Biochemical genetics 27 (1989), S. 219-228 
    Details
    ISSN: 1573-4927
    Keywords: thymidine kinase ; galactokinase ; fibroblasts ; cell culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The structural genes for human galactokinase (GALK) and the human cytosolic form of thymidine kinase (TK1) are located on 17q21–q22. These two loci are tightly linked, and studies on Chinese hamster cell lines have shown that the expression of TK1 and GALK genes may alter simultaneously. We investigated the possibility of a dependent mutation of TK1 and GALK genes in cultured fibroblasts obtained from two patients homozygous for the GALKG-deficient gene. Since we showed that the TK1 level varies as a function of the passage and the growth rate of a given strain, our experiments were performed on nonstored skin fibroblasts, between the third and the fifth passage for both controls and patients. We found that TK1 levels in GALK-deficient cells were almost 75% of those observed in control strains with a similar growth rate. Previous results in the literature have shown a pronounced decrease in TK1 activity in three GALK-deficient fibroblastic strains. We suggest that these disparities of TK1 levels in GALK-deficient fibroblasts may be related either to genetic heterogeneity of GALK deficiency or to differences in culture conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00552993
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Cytosolic thymidine kinase activity in cultured human fibroblasts from individuals with galactokinase deficiency (1989)
    Springer
    Biochemical genetics 27 (1989), S. 219-228 
    Details
    ISSN: 1573-4927
    Keywords: thymidine kinase ; galactokinase ; fibroblasts ; cell culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The structural genes for human galactokinase (GALK) and the human cytosolic form of thymidine kinase (TK1) are located on 17q21–q22. These two loci are tightly linked, and studies on Chinese hamster cell lines have shown that the expression of TK1 and GALK genes may alter simultaneously. We investigated the possibility of a dependent mutation of TK1 and GALK genes in cultured fibroblasts obtained from two patients homozygous for the GALKG-deficient gene. Since we showed that the TK1 level varies as a function of the passage and the growth rate of a given strain, our experiments were performed on nonstored skin fibroblasts, between the third and the fifth passage for both controls and patients. We found that TK1 levels in GALK-deficient cells were almost 75% of those observed in control strains with a similar growth rate. Previous results in the literature have shown a pronounced decrease in TK1 activity in three GALK-deficient fibroblastic strains. We suggest that these disparities of TK1 levels in GALK-deficient fibroblasts may be related either to genetic heterogeneity of GALK deficiency or to differences in culture conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02401802
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Mullite based compositions prepared by sol-gel techniques (1997)
    Springer
    Journal of sol gel science and technology 8 (1997), S. 403-407 
    Details
    ISSN: 1573-4846
    Keywords: alumina-silica ; multite ; composites ; gels and powders
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Ceramic powders and gels in the multi-alumina system with different compositions were prepared by sol-gel method. The preparation of gels was done in strong acidic conditions and also with the addition of ammonia. The powders were prepared by dropping the sols on a hot plate. The effect of water/alkoxide and ethanol/alkoxide ratios was studied together with the type of catalyst used (hydrochloric acid or ammonia). The thermal evolution of materials was followed by thermal analysis, X-ray diffraction, specific surface area measurements and electron microscopy. The morphology of powders was also studied. The characteristic exothermic peak attributed to a good mixing degree of the Al and Si species was observed at ∼980°C. The intensity of this exothermic peak was more pronounced in gels than in sol-gel derived powders. The crystalline phases formed are related with the preparation conditions and with the thermal treatment. The crystallisation of mullite and transient phases of alumina were observed in powder samples heat treated at 1000°C while in gel samples mullite was the only crystalline phase detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02436873
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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