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  • ceruloplasmin receptor  (1)
  • α-1-antitrypsin  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Expression of the human α-1-antitrypsin gene in transgenic rats (1995)
    Springer
    Bulletin of experimental biology and medicine 120 (1995), S. 727-729 
    Details
    ISSN: 1573-8221
    Keywords: α-1-antitrypsin ; transgenic animals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A genetic-engineering construction is developed to provide expression of the full-size cDNA of human α-1-antitrypsin controlled by the promoter elements of the murine metallothionein-1 gene. Transgenic animals were obtained by microinjection of pAT-MT plasmid into fertilized rat oocytes. The genome of such animals contains the sequences of human α-1-antitrypsin-cDNA revealed by blot- and dot-hybridization. Integrated human α-1-antitrypsin-cDNA copies are found with a high frequency (9 out of 22 animals) in the second-generation offspring obtained after crossings of transgenic animals. A protein with antigenic specificity of human a-1-antitrypsin is found in the serum of transgenic rats by the immunoblotting method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02444673
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation and partial characterization of cDNA clone of human ceruloplasmin receptor (2000)
    Springer
    Bulletin of experimental biology and medicine 129 (2000), S. 491-495 
    Details
    ISSN: 1573-8221
    Keywords: ceruloplasmin receptor ; cloning ; cDNA sequencing ; copper metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract An individual clone, presumably carrying a 3 bp fragment of ceruloplasmin receptor cDNA was isolated from the expression library of human placenta cDNA using polyclonal specific antibodies to ceruloplasmin receptors.EcoR1-hydrolysate of isolated DNA was cloned in apTZ19 bacterial vector and sequenced in the forward and reverse direction. The comparison of the revealed sequence with known sequences of human genome revealed its high similarity to ceruloplasmin cDNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02439812
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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