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  • 1
    ISSN: 1572-8889
    Keywords: honeybee ; learning ; proboscis extension ; olfaction ; odor mixture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Individually restrained worker bees were trained to recognize complex odors in a conditioned proboscis extension assay. Three groups of bees were considered, based on the responses recorded during the experimental procedure: selective learners, nonselective learners, and nonlearners. For conditioning, three concentrations of two synthetic mixtures were used. The distribution of bees between groups was not significantly affected by the nature or by the concentration of the conditioning mixture. After conditioning, bees were tested with the individual compounds, and the responses were analyzed with respect to the three groups. Selective learners showed discriminative responses to a few key compounds, while nonselective learners responded to all the compounds, and nonlearners to none. These results showed that complex odor recognition is based on the recognition of key components and relies on the ability of bees to learn.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of chemical ecology 16 (1990), S. 701-711 
    ISSN: 1573-1561
    Keywords: Insect-plant relationships ; olfaction ; honeybee ; Hymenoptera ; Apidae ; sunflower ; allelochemicals ; coupling GC-EAG
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Combined electrophysiological recordings (EAG) and gas chromatographic separation were performed in order to investigate which volatile chemical components of a sunflower extract could be detected by honeybee workers and thus are likely to trigger the foraging behavior. A direct coupling device allowed for the stimulation of the antennal receptors with individual constituents of a polar fraction of the flower aroma shown to be attractive to bees. More than 100 compounds were separated from the extract. Twenty-four compounds elicited clear EAG responses. These compounds were identified by mass spectrometry (electronic impact and chemical ionisation). Both short- and long-chain aliphatic alcohols, one short-chain aliphatic aldehyde, one acid, two esters, and terpenic compounds were found to stimulate the antennal receptors. Six compounds identified in previous behavioral experiments were found to exhibit EAG activity. The chemicals screened by this method may be used for recognition of the plant odor and the selective behavior of honeybees.
    Type of Medium: Electronic Resource
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