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Digitale Medien

High cell density perfusion cultures of anchorage-dependent Vero cells in a depth filter perfusion system (1995)

Choi, Sang Kyo ; Chang, Ho Nam ; Lee, Gyun Min ; [weitere]

Springer

Cytotechnology 17 (1995), S. 173-183

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ISSN: 1573-0778

Schlagwort(e): depth filter perfusion system ; Vero cell culture ; gelatin coating ; polypropylene fiber ; air sparging ; high cell density culture

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Abstract A depth filter perfusion system (DFPS) with polypropylene fibers had been demonstrated to support high density cultures of anchorage-independent hybridoma cells. The DFPS provides advantages of high surface-to-volume ratio of 450–600 cm2/cm3, low cost set-up, easy operation and scale-up. To test the feasibility of using DFPS for high density cultures of anchorage-dependent cells, Vero cells were cultivated in the DFPS. Gelatin coating on polypropylene fibers in the DFPS was necessary to promote cell attachment and growth. Dissolved oxygen (DO) concentrations could be controlled by sparging air into the reservoir vessel through a filter sparger. When DO concentration was controlled above 40% of air saturation in the DFPS with 40 μm pore size, the maximum cell concentration as estimated on specific lactate production rate, was 3.81×107 cells/ml of the total reactor volume. This viable cell concentration is approximately 18 times higher than that obtained in a T-flask batch culture. Taken together, the results obtained here showed the potential of DFPS for high-density cultures of anchorage-dependent cells.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00749655

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Volltext

Digitale Medien

High-density continuous cultures of hybridoma cells in a depth filter perfusion system (1994)

Oh, Duk Jae ; Choi, Sang Kyo ; Chang, Ho Nam

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 44 (1994), S. 895-901

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ISSN: 0006-3592

Schlagwort(e): hybridoma culture ; monoclonal antibody production ; depth filter perfusion system ; perfusion ulture ; Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: A depth filter perfusion system (DFPS) for animal cell culture was developed and its use in continuous highdensity cultures of hybridoma cells was investigated. In the DFPS, based on cell immobilization in a cylindrical depth filter matrix, cells were easily immobilized and cultivated by simple medium recirculation. The cell density in the 20-μm pore size filter matrix reached up to 3 × 107 cells/mLin less than 10 days. This resulted in a high monoclonal antibody productivity of 744 mg/L/day, which was 25-35 times higher than that of continuous-suspension cultures using the same cell line. The 20-μm pore filter retained more cells than the 30-μm filterin a shorter period. The DFPS provides advantages of low-cost set-up, easy operation, and scale-up in the cultures of anchorage-independent cells. It also has a high potential for anchorage-dependent cell cultures because of its unusually high surface-to-volume ratio of 450-600 cm2/cm3. © 1994 John Wiley & Sons, Inc.

Zusätzliches Material: 7 Ill.