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  • 1
    Electronic Resource
    Electronic Resource
    High cell density perfusion cultures of anchorage-dependent Vero cells in a depth filter perfusion system (1995)
    Springer
    Cytotechnology 17 (1995), S. 173-183 
    Details
    ISSN: 1573-0778
    Keywords: depth filter perfusion system ; Vero cell culture ; gelatin coating ; polypropylene fiber ; air sparging ; high cell density culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A depth filter perfusion system (DFPS) with polypropylene fibers had been demonstrated to support high density cultures of anchorage-independent hybridoma cells. The DFPS provides advantages of high surface-to-volume ratio of 450–600 cm2/cm3, low cost set-up, easy operation and scale-up. To test the feasibility of using DFPS for high density cultures of anchorage-dependent cells, Vero cells were cultivated in the DFPS. Gelatin coating on polypropylene fibers in the DFPS was necessary to promote cell attachment and growth. Dissolved oxygen (DO) concentrations could be controlled by sparging air into the reservoir vessel through a filter sparger. When DO concentration was controlled above 40% of air saturation in the DFPS with 40 μm pore size, the maximum cell concentration as estimated on specific lactate production rate, was 3.81×107 cells/ml of the total reactor volume. This viable cell concentration is approximately 18 times higher than that obtained in a T-flask batch culture. Taken together, the results obtained here showed the potential of DFPS for high-density cultures of anchorage-dependent cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00749655
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  • 2
    Electronic Resource
    Electronic Resource
    Continuous production of tissue plasminogen activator from recombinant CHO cells in a depth filter perfusion system (1995)
    Springer
    Biotechnology techniques 9 (1995), S. 567-572 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A depth filter perfusion system (DFPS), equipped with a 40-μm polypropylene depth filter for cell immobilization, was used for the continuous production of tissue plasminogen activator (t-PA) from recombinant Chinese hamster ovary cells. Final cell density in the DFPS with oxygen control was 1.8×107 cells/mL of the total working volume and maximum t-PA productivity was 2.63 mg/L/day. Dissolved oxygen concentration in the filter matrix was successfully controlled by air sparging and stable operation was possible for more than 20 days.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00152445
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  • 3
    Electronic Resource
    Electronic Resource
    Production of monoclonal antibodies against human chorionic gonadotropin by hybridoma cultures in calcium alginate capsules (1993)
    Springer
    Cytotechnology 13 (1993), S. 41-49 
    Details
    ISSN: 1573-0778
    Keywords: calcium-alginate capsule ; hybridoma culture ; microencapsulation ; monoclonal antibody production ; oxygen transfer analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A new method of making microcapsules with calcium alginate gel was developed and the cultivation of the encapsulated hybridoma cells producing monoclonal antibodies against human chorionic gonadotropin was investigated. A high cell density of 2.0×108 cells/cm3 in the capsules led to a high dilution rate of 0.68 per hour and resulted in the high volumetric monoclonal antibody productivity of 652.8 mg/l/day, which was 20–30 times higher than those of traditional continuous suspension cultures. However, long-term continuous culture was not achieved with this capsule system probably because of the limitation in nutrient supply and the accumulation of waste products. Also the analysis of oxygen transfer in this system showed that oxygen supply was not enough to support such a high cell density.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00749974
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  • 4
    Electronic Resource
    Electronic Resource
    High-density continuous cultures of hybridoma cells in a depth filter perfusion system (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 44 (1994), S. 895-901 
    Details
    ISSN: 0006-3592
    Keywords: hybridoma culture ; monoclonal antibody production ; depth filter perfusion system ; perfusion ulture ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A depth filter perfusion system (DFPS) for animal cell culture was developed and its use in continuous highdensity cultures of hybridoma cells was investigated. In the DFPS, based on cell immobilization in a cylindrical depth filter matrix, cells were easily immobilized and cultivated by simple medium recirculation. The cell density in the 20-μm pore size filter matrix reached up to 3 × 107 cells/mLin less than 10 days. This resulted in a high monoclonal antibody productivity of 744 mg/L/day, which was 25-35 times higher than that of continuous-suspension cultures using the same cell line. The 20-μm pore filter retained more cells than the 30-μm filterin a shorter period. The DFPS provides advantages of low-cost set-up, easy operation, and scale-up in the cultures of anchorage-independent cells. It also has a high potential for anchorage-dependent cell cultures because of its unusually high surface-to-volume ratio of 450-600 cm2/cm3. © 1994 John Wiley & Sons, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260440805
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