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  • 1
    ISSN: 1573-5060
    Keywords: bean common mosaic virus (BCMV) ; bean common mosaic necrosis virus(BCMNV) ; epistatic resistance genes ; gene pyramiding ; Phaseolus vulgaris ; SCAR marker
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Pyramiding epistatic resistance genes to improve long term disease resistance has challenged plant breeders. Indirect selection using tightly linked markers will often facilitate the breeding of desired epistatic resistance gene combinations. In common bean, the most effective strategy for broad spectrum control of the bean common mosaic virus disease is to combine I, bc-u, bc-1 2, bc-2 2, and bc-3 genes. We describe the use of near-isogenic lines and bulked segregant analysis to identify a marker tightly linked with the bc-1 2 gene. The recessive bc-1 2 gene conditions resistance to specific strains of bean common mosaic virus and bean common mosaic necrosis virus and is masked by the bc-2 2 and bc-3 genes. We identified a RAPD marker completely linked (0 recombinants) with bc-1 2, based on 72 F3 progeny generated from a cross between the contrasting near isogenic lines (I + bc-1/I + bc-1 2). Segregation in this I gene background revealed that bc-1 2 was dominant to bc-1 in conferring resistance to top necrosis in the allelic series Bc-1 〉 bc-1 2 〉 bc-1. To facilitate marker-assisted selection of bc-1 2 across breeding programs, the RAPD was converted to a SCAR marker, designated SBD51300. Tight linkage (0 recombinants) was confirmed in a second population of 58 F2 progeny co-segregating for SBD51300 and bc-1 2 gene from a different source. Based on a survey of 130 genotypes, the SCAR will be useful for MAS of bc-1 2 in most beans of Middle American origin and snap beans, but will have very limited utility in the case of kidney and cranberry beans. The SBD51300 marker mapped on linkage group B3, revealing independence of bc-1 2 from the I gene on B2 and bc-3 gene on B6, which supports the opportunity to readily combine genes for broad spectrum and pyramided resistance to bean common mosaic potyviruses in a single bean cultivar.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 102 (1998), S. 343-356 
    ISSN: 1573-5060
    Keywords: breeding pyramid ; combining ability ; gene pools ; ideotype ; partitioning ; wild species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Strategies employed by dry bean breeders to improve yield include early generation testing, ideotype breeding, selection for physiological efficiency, and selection based on genotypic performance and combining ability across gene pools of Phaseolus vulgaris. Ideotype breeding has been successfully deployed to improve yield in navy, pinto and great northern seed types. The ideotype method is based on an ideal plant architecture to which breeders target their selection. Breeding for physiological efficiency is important in combining increased biomass, high growth rates and efficient partitioning. Genotypic performance and combining ability are also critical for yield improvement, since crosses between gene pools can exhibit negative combining ability and problems with lethality, whereas interracial crosses within the same gene pool exhibit the greatest potential. Breeders must work within specific constraints for growth habit, maturity, seed quality and disease resistance. A three-tiered pyramidal breeding strategy is proposed to facilitate yield improvement in dry bean. Breeding of elite, agronomically acceptable germplasm within the same market class is restricted to the apex of the pyramid. The intermediate level has fewer constraints and greater access to diverse germplasm. Interracial crosses within the same gene pool are utilized to exploit genetic differences within adapted material. Extracting genetic diversity from unadapted sources, including wild germplasm and other Phaseolus species, is conducted at the base of the pyramid. The objective of this breeding strategy is the movement of improved germplasm towards the apex, using different breeding procedures to optimize improvement at each tier of the breeding pyramid.
    Type of Medium: Electronic Resource
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