ISSN:
0021-9304
Keywords:
cell-substrate interaction
;
cell adhesion
;
cell repulsion
;
guidance
;
poly(ethylene glycol)
;
hippocampal neurons
;
patterning
;
peptides
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
,
Technology
Notes:
Glass surfaces were patterned with cell-adhesive regions of laminin adhesive peptides YIGSR, RGD, and IKVAV, and cell-repulsive regions of poly(ethylene glycol) (PEG). The patterns were created by sputter-coating titanium and then gold onto glass coverslips through electron microscope grids. Gold surfaces were modified with cysteine-terminated peptides to have approximately 450 fmol/cm2 of peptide incorporated on the glass coverslips as determined with radiolabeled CGYIGSR. Amine-functionalized glass coverslips were prepared using an amine-functionalized silane and then further modified with PEG-aldehyde by a Schiff base reduction. All surfaces were characterized by X-ray photoelectron spectroscopy and water contact angles. Hippocampal neurons, plated from a serum-free medium, adhered preferentially to peptide-functionalized surfaces over PEG-modified surfaces. Cell adhesion and neurite outgrowth were limited to the peptide region, demonstrating that neurite outgrowth could be directed by a combination of cell-adhesive and cell-repulsive cues. © 1998 John Wiley & Sons, Inc. J. Biomed Mater Res, 42, 13-19, 1998.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
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