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  • 1
    Electronic Resource
    Electronic Resource
    Photosynthetically active suspension cultures of potato spindle tuber viroid infected tomato cells as tools for studying viroid — host cell interaction (1993)
    Springer
    Plant cell reports 12 (1993), S. 597-602 
    Details
    ISSN: 1432-203X
    Keywords: Lycopersicon peruvianum ; Lycopersicon esculentum ; viroid pathogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Photosynthetically active callus and cell suspension cultures were established from uninfected Lycopersicon peruvianum plants and from uninfected and potato spindle tuber viroid (PSTVd) infected plants of Lycopersicon esculentum cv. Rutgers. Viroid infection was maintained in photoheterotrophic culture on media containing 3% sucrose, but during continuous photo-mixotrophic culture in low sucrose media (1% sucrose), the level of PSTVd accumulation decreased. Photoautotrophic cell suspensions could be established with uninfected, but not with viroid infected tomato cells. As compared to uninfected cells, PSTVd infected cells grew slowly, were morphologically different in size and shape, and formed tight cell aggregates. Electronmicroscopy showed that starch accumulation in chloroplasts, deformation of the chloroplast envelope and irregular plasmalemmasomes at the cell membrane were associated with PSTVd infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232806
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Contitions for optimal growth of a PSTV-infected potato cell suspension and detection of viroid-complementary longer-than-unit-length RNA in these cells (1983)
    Springer
    Plant molecular biology 2 (1983), S. 239-247 
    Details
    ISSN: 1573-5028
    Keywords: molecular hybridization ; plant cell cultures ; potato spindle tuber viroid (PSTV) ; viroid replication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A suspension culture from potato spindle tuber viroid (PSTV)-infected cells of the wild type potato (Solanum demissum) has been established, which is a suitable model system for studying PSTV replicationin vivo. The conditions for rapid growth of these cells and for permanent extensive viroid biosynthesis within them are described. Biosynthesis of PSTV in the potato cells was demonstrated by32P-incorporation into nucleic acids and their subsequent electrophoretic analysis on polyacrylamide gels. Under optimum culture conditions the amount of32P-orthophosphate incorporation into PSTV reached 10% of that incorporated into the 2 M LiCl-soluble cellular RNA. (+)PSTV and its complementary form, i.e. (−)PSTV were identified after their electrophoretic separation on polyacrylamide and agarose gels by molecular hybridization. This analysis revealed the presence of six high molecular weight(−)PSTV species, which are possibly multimers of the unit length(+)PSTV molecule consisting of 359 nucleotides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01578642
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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