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Digitale Medien

A study of nicotine release from tobacco hairy roots by transient technique (1993)

Larsen, William A. ; Hsu, James T. ; Flores, Hector E. ; [weitere]

Springer

Biotechnology techniques 7 (1993), S. 557-562

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Details

ISSN: 1573-6784

Quelle: Springer Online Journal Archives 1860-2000

Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Abstract A material balancing method for determining the productivity of nicotine-releasing Nicotiana tabacum hairy roots is presented. Diffusive nicotine release from the intracellular environment of N. tabacum to its medium is based upon an equilibrium partitioning process. By removing nicotine from a culture's medium, subsequent transient release from the tissue is induced, as is the heightened production of nicotine. Under normal conditions, sixteen hours is required for releasing tissue to re-establish equilibrium with the medium. 100 ppm of the surfactant Triton X-100 was used to enhance the rate of nicotine release and to reduce the return to equilibrium to four hours without toxic side-effects.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00156328

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