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  • 11
    Electronic Resource
    Electronic Resource
    Analysis ofcis-regulatory elements involved in induction of a tobacco PR-5 gene by virus infection (1992)
    Springer
    Plant molecular biology 18 (1992), S. 155-158 
    Details
    ISSN: 1573-5028
    Keywords: pathogenesis-related proteins ; tobacco mosaic virus infection ; cis-regulatory elements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract cis-Regulatory elements involved in tobacco mosaic virus (TMV)-inducible expression were indentified in a tobacco PR-5 gene, encoding an acidic thaumatin-like protein. By fusing upstream sequences of the PR-5 gene to the GUS reporter gene and analysing transgenic plants containing these fusions for local and systemic induction of GUS activity by TMV, it was found that sequences between-1364 and-718 are involved in TMV induction of PR-5 gene expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00018471
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  • 12
    Electronic Resource
    Electronic Resource
    The PR-1a promoter contains a number of elements that bind GT-1-like nuclear factors with different affinity (1996)
    Springer
    Plant molecular biology 30 (1996), S. 493-504 
    Details
    ISSN: 1573-5028
    Keywords: GT-1 ; induced expression ; PR proteins ; salicylic acid ; trans-acting factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The 900 by promoter region of the tobacco PR-1a gene was divided into eight fragments using PCR. The fragments were tested for their ability to bind to nuclear factors isolated from tobacco leaf. Band shift assays demonstrated that all but one of the fragments specifically interacted with nuclear proteins. From competition experiments it was determined that the same nuclear factors bind various promoter fragments with different affinity. Moreover, efficient competition with a synthetic tetramer of box II of the rbcS promoter (Green PJ et al., EMBO J 13 (1988) 4035–4044) indicated that GT-1-like nuclear factors are involved in these interactions. Furthermore, in comparison to extracts from untreated plants, nuclear protein preparations from tobacco mosaic virus-infected tobacco showed a reduced GT-1 binding activity. These results will be discussed in relation to induced PR-1a gene expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00049327
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  • 13
    Electronic Resource
    Electronic Resource
    Biologically active transcripts of cloned DNA of the coat protein messenger of two plant viruses (1986)
    Springer
    Plant molecular biology 6 (1986), S. 281-288 
    Details
    ISSN: 1573-5028
    Keywords: alfalfa mosaic virus ; tobacco streak virus ; in vitro translation ; in vitro transcription ; in vivo translation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To initiate infection, a mixture of the three genomic RNAs of alfalfa mosaic virus (AIMV) has to be supplemented with a small amount of coat protein or RNA 4, the subgenomic messenger for coat protein. The possibility to replace RNA 4 in the inoculum by in vitro synthesized transcripts of a cloned DNA copy of the coat protein cistron was investigated using the SP6 transcription system. Transcripts with or without the cap structure m7G(5′)ppp(5′)G were both translated in vitro in viral coat protein, but only capped transcripts yielded an infectious mixture when added to the AIMV genomic RNAs. This indicates that the cap structure is essential to the in vivo translatin of RNA 4. Similar results were obtained with RNAs transcribed in vitro from a DNA copy of the putative coat protein cistron of tobacco streak virus (TSV). re]19850822 rv]19851203 ac]19860114
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015234
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  • 14
    Electronic Resource
    Electronic Resource
    Transformation of lily by Agrobacterium (1955)
    Springer
    Euphytica 85 (1955), S. 97-100 
    Details
    ISSN: 1573-5060
    Keywords: Agrobacterium ; transformation ; lily ; β-glucuronidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Lily cv. Harmony was inoculated with several Agrobacterium strains to study its susceptibility to Agrobacterium infection and transformation. Tumorous tissue formation on inoculated stem internodes of sterile-grown plantlets, as well as expression of a β-glucuronidase marker gene interrupted by an intron in cells of inoculated stem nodes, indicate that the monocotyledon Lilium is a host for Agrobacterium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023935
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  • 15
    Electronic Resource
    Electronic Resource
    Differential induction of acquired resistance and PR gene expression in tobacco by virus infection, ethephon treatment, UV light and wounding (1991)
    Springer
    Plant molecular biology 17 (1991), S. 1117-1125 
    Details
    ISSN: 1573-5028
    Keywords: β-1,3-glucanase ; acquired resistance ; chitinase ; phenylalanine ammonia-lyase ; wound-induced proteins ; UV-induced proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genes for acidic, extracellular and basic, intracellular pathogenesis-related (PR) proteins of tobacco were studied for their response to tobacco mosaic virus (TMV) infection, ethephon treatment, wounding and UV light. The genes encoding the acidic PR proteins (PR-1, PR-2, PR-3, PR-4 and PR-5) responded similarly to the different forms of stress. They appeared to be highly inducible by TMV, moderately inducible by ethephon treatment and UV light and not inducible by wounding. The genes for the basic counterparts of PR-1, PR-2, PR-3 and PR-5 also displayed a common stress response. However, this response was different from that of the acidic PR proteins. Here, the highest induction was obtained upon ethephon treatment, while the other stress conditions resulted in somewhat lower levels of expression. Most genes for acidic PR proteins are systemically induced in the uninfected upper leaves of TMV-infected plants, whereas the genes encoding the basic PR proteins are not. Increased levels of resistance to TMV, comparable to resistance obtained by pre-infection with the virus, were found in UV-irradiated leaves but not in wounded or ethephon-treated leaves. This indicates that the basic PR proteins are not involved in resistance to TMV infection. Tobacco phenylalanine ammonia-lyase genes were not inducible by the various stress conditions. The implications of these findings in relation to the phenomenon of acquired resistance are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028729
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  • 16
    Electronic Resource
    Electronic Resource
    Circadian expression and induction by wounding of tobacco genes for cysteine proteinase (1993)
    Springer
    Plant molecular biology 21 (1993), S. 685-694 
    Details
    ISSN: 1573-5028
    Keywords: circadian expression ; papain ; targeting ; thiol protease ; wound induction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two sets of clones were isolated from a tobacco cDNA library, utilizing as a probe a PCR fragment obtained from tomato cDNA using a degenerate primer based on the sequence of tomato systemin. Contrary to expectation, the clones did not correspond to tobacco homologues of tomato pro-systemin. However, the cDNAs encoded two highly similar proteins with extensive structural homology to cysteine proteinases from a wide range of plant and animal species. Northern blot analyses showed that in unstressed tobacco leaf the genes for the putative proteinases are expressed according to a circadian rhythm. Furthermore, incision wounding enhances the expression approximately six-fold. Other forms of stress, such as infection with tobacco mosaic virus, treatment with ethephon or UV light do not result in induced expression of the tobacco cysteine proteinase genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00014551
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  • 17
    Electronic Resource
    Electronic Resource
    Analysis of regulatory elements involved in stress-induced and organ-specific expression of tobacco acidic and basic β-1,3-glucanase genes (1993)
    Springer
    Plant molecular biology 21 (1993), S. 451-461 
    Details
    ISSN: 1573-5028
    Keywords: β-1,3-glucanase ; organ-specific expression ; pathogenesis-related proteins ; regulatory elements ; tobacco mosaic virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Infection of tobacco by tobacco mosaic virus (TMV) induces coordinate expression of genes encoding acidic and basic β-1,3-glucanase isoforms. These genes are differentially expressed in response to other treatments. Salicylate treatment induces acidic glucanase mRNA to a higher level than basic glucanase mRNA. Ethylene treatment and wounding strongly induce the basic glucanase genes but have little effect on genes encoding the acidic isoforms. Furthermore, the basic glucanase genes are constitutively expressed in roots and lower leaves of healthy plants, whereas the acidic glucanase genes are not. In order to investigate how these expression patterns are established, we fused promoter regions of an acidic and a basic glucanase gene to the β-glucuronidase (GUS) reporter gene and examined expression of these constructs in transgenic tobacco plants. A fragment of 1750 bp and two 5′-truncated fragments of 650 bp and 300 bp of the acidic glucanase promoter were tested for induction of GUS gene expression after salicylate treatment and TMV infection. Upstream sequences of 1750 bp and 650 bp were sufficient for induction of the reporter gene by salicylate treatment and TMV infection, but the activity of the 300 bp fragment was strongly reduced. The results suggest that the 1750 bp upstream sequence of the acidic glucanase gene contains multiple regulatory elements. For the basic glucanase promoter it is shown that 1476 bp of upstream sequences were able to drive expression in response to TMV infection and ethylene treatment, but no response was found to incision wounding. Furthermore, high GUS activity was found in lower leaves and roots of healthy transgenic plants, carrying the 1476 bp basic glucanase promoter/GUS construct. When the promoter was truncated up to position −446 all activity was lost, indicating that the region between −1476 and −446 of the basic glucanase promoter is necessary for organ-specific and developmentally regulated expression as well as for induced expression in response to infection and other stress treatments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028803
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  • 18
    Electronic Resource
    Electronic Resource
    Homology between the proteins encoded by tobacco mosaic virus and two tricornaviruses (1984)
    Springer
    Plant molecular biology 3 (1984), S. 379-384 
    Details
    ISSN: 1573-5028
    Keywords: alfalfa mosaic virus ; brome mosaic virus ; tobacco mosaic virus ; protein homologies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A comparison was made of the amino acid sequences of the proteins encoded by RNAs 1 and 2 of alfalfa mosaic virus (A1MV) and brome mosaic virus (BMV), and the 126K and 183K proteins encoded by tobacco mosaic virus (TMV). Three blocks of extensive homology of about 200 to 350 amino acids each were observed. Two of these blocks are located in the A1MV and BMV RNA 1 encoded proteins and the TMV encoded 126K protein; they are situated at the N-terminus and C-terminus, respectively. The third block is located in the A1MV and BMV RNA 2 encoded proteins and the C-terminal part of the TMV encoded 183K protein. These homologies are discussed with respect to the functional equivalence of these putative replicase proteins and a possible evolutionary connection between A1MV, BMV and TMV.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033385
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