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  • 11
    Electronic Resource
    Electronic Resource
    Effects of Pluronic F-68 on plant cells in suspension culture (1990)
    Springer
    Biotechnology letters 12 (1990), S. 29-32 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The effects of the non-ionic surfactant, Pluronic F-68, on growth and structure ofSolanum dulcamara cells in suspension culture have been studied. Growth of cells, as measured by dry weight, was unaffected by low concentrations (0.01–1.0% w/v) of pluronic, while culture with higher concentrations (2.5–10.0%) resulted in cell death. It is suggested that low concentrations of pluronic may be valuable supplements in plant cell cultures to protect against mechanical damage and to manipulate membrane systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01028488
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    Paper (German National Licenses)
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  • 12
    Electronic Resource
    Electronic Resource
    Parameters influencing transient and stable transformation of barley (Hordeum vulgare L.) protoplasts (1995)
    Springer
    Plant cell, tissue and organ culture 41 (1995), S. 125-138 
    Details
    ISSN: 1573-5044
    Keywords: Hordeum vulgare ; plasmid uptake ; polyethylene glycol (PEG) ; protoplasts ; transient gene expression ; stable transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cat gene expression has been investigated following PEG-mediated plasmid uptake into barley protoplasts. The uptake conditions optimised for transient expression were employed for stable transformation. Transformed protoplast-derived calli of the cvs. Dissa and Igri, were selected on medium containing G418 at 40 μg ml−1 or kanamycin sulphate at 250 μg ml−1. Absolute transformation frequencies of 28.9×10−5 and 21.3×10−5 were recorded for Dissa with kanamycin sulphate and G418 selection, respectively. The frequency for Igri was 11.5×10−5 with G418 selection. Antibiotic resistant protoplast-derived colonies expressed NPTII activity; Southern hybridisation confirmed integration of the nptII gene into barley genomic DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00051581
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    Paper (German National Licenses)
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  • 13
    Electronic Resource
    Electronic Resource
    Effects of Ri plasmid rol gene expression on the IAA content of transformed roots of Solanum dulcamara L. (1991)
    Springer
    Plant growth regulation 10 (1991), S. 235-241 
    Details
    ISSN: 1573-5087
    Keywords: rol genes ; hairy roots ; auxins ; plant growth regulation ; Solanum dulcamara
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects have been investigated of various combinations of rol genes, together with Ri plasmid TR-DNA transcripts 1 and 2, upon free IAA levels in transformed roots of Solanum dulcamara L. Differences between the IAA levels were observed, including a highly significant (p=0.01) difference between the IAA levels of rol − TR+ and rol ABC+ TR+ transformed roots. Possible mechanisms are discussed for the regulation of free IAA levels by rol gene products.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024414
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    Paper (German National Licenses)
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  • 14
    Electronic Resource
    Electronic Resource
    Effects of Pluronic F-68 on shoot regeneration from cultured jute cotyledons and on growth of transformed roots (1993)
    Springer
    Plant cell, tissue and organ culture 34 (1993), S. 133-140 
    Details
    ISSN: 1573-5044
    Keywords: Corchorus capsularis ; medium supplement ; necrosis ; organogenesis ; suppression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects have been studied of the non-ionic surfactant, Pluronic F-68, on the growth in culture of jute (Corchorus capsularis L.) cotyledons with attached petioles, cotyledon explants and transformed roots. Supplementation of culture medium with 0.001–0.5% (w/v) of either commercial grade Pluronic F-68 or a purified fraction prepared by passage through silica gel, stimulated shoot production from the petioles of C. capsularis var. D154 and C134 cotyledons. This effect was most marked in C134, because of the failure of control cotyledons to produce shoots in the absence of Pluronic. Plants regenerated from Pluronic-treated cotyledons were morphologically normal. Growth of transformed roots of C. capsularis var. D154 was stimulated in medium supplemented with commercial grade or purified Pluronic F-68, with maximum increases in both fresh and dry weights with 0.1% (w/v) of the surfactant. Roots cultured in the presence of Pluronic F-68 could be maintained without sub-culture for up to 70 days, whereas roots cultured in the absence of Pluronic required subculture every 7 days, to prevent necrosis. Transformed roots also produced callus in the presence of 0.001–1.0% (w/v) of either commercial grade or purified Pluronic. The biotechnological implications of these results are discussed in relation to the potential value of non-ionic surfactants as growth-stimulating additives to plant culture media.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036093
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  • 15
    Electronic Resource
    Electronic Resource
    Effects of Pluronic F-68 on growth of transformed roots ofSolanum dulcamara (1990)
    Springer
    Biotechnology letters 12 (1990), S. 937-940 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The effects of the non-ionic surfactant, Pluronic F-68, on the growth of transformed roots ofSolanum dulcamara L. have been studied. Growth was stimulated by addition of low concentrations (0.001–0.1% w/v) of freshly-prepared commercial grade Pluronic to liquid culture medium, with maximum increases in root fresh and dry weights at 0.01% (w/v). In contrast, higher concentrations (0.25–1.00% w/v) of freshly-prepared Pluronic inhibited growth. Freshly-prepared purified Pluronic retarded root growth, even at concentrations that were stimulatory with the commercial preparation. Similarly, commercial grade Pluronic solutions stored at 4°C or 22°C for 5 days (“aged”) were inhibitory to root growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01022594
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    Paper (German National Licenses)
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