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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Journal of comparative physiology 80 (1972), S. 247-254 
    ISSN: 1432-1351
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Naupliar brine shrimp (Artemia salina) have been used to study the differentiation of salt secretory cells. The effects of several kinds of inhibitors on survival of nauplii at various salinities are presented. Results with ouabain indicate that the ability of the nauplii to survive at high salinities is dependent upon the presence of Na++K+-activated ATPase. Puromycin, chloramphenicol and cycloheximide, all inhibitors of protein synthesis, are more effective at high salt concentrations. Inhibitors of RNA synthesis that are primarily extranuclear in mechanism, i.e. rifamycin and ethidium bromide, also show a marked salt dependency. Among inhibitors which were assayed but did not effect survival under any circumstances were mitomycin C, 5-fluorodeoxyuridine and sarkomycin. Those inhibitors which did not show a differential salt sensitivity were actinomycin D and α-amanitin. It is concluded from these data that coincident transcription and translation of both nuclear and mitochondrial genes are involved in the development of the naupliar salt gland.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Journal of comparative physiology 82 (1973), S. 277-289 
    ISSN: 1432-1351
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Naupliar brine shrimp (Artemia salina) have been used to study the salt-dependent regulation of protein synthesis. Measurement of thein vivo rates of protein synthesis was found to be very complex and dependent upon the leucine concentration of the external medium, rate of leucine entry and time of equilibration between various internal pools of leucine. Techniques were developed which permitted the measurement of rates of incorporation ofl4C-L-leucine into naupliar protein at various salinities under conditions that provided the organisms with a constant internal specific activity. It was found that salinities over 0.25 M NaC1 caused decreased rates of protein biosynthesis. A comparison of the rate of protein synthesis in the presence of chloramphenicol, cycloheximide and puromycin indicated that qualitative as well as quantitative changes in synthesis of proteins was directed by the external salinity. A feed-back mechanism based on the partitioning of available energy (ATP) between ion transport and protein synthesis is hypothesized.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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