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  • 11
    Electronic Resource
    Electronic Resource
    An Effect of Ultrasonic Standing Waves on Electrodeposition (1965)
    [s.l.] : Nature Publishing Group
    Nature 207 (1965), S. 186-186 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Ultrasonic standing waves were set up in the electrolyte of a small plating cell, using a barium titanate disk transducer and, 2*5 cm from it, a glass block as a reflector. In the gap between the reflector and transducer was a sheet nickel cathode, 1-5 cm x 1 cm, arranged so that the standing ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/207186a0
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  • 12
    Electronic Resource
    Electronic Resource
    Identification of canary grass (Phalaris aquatica) pollen allergens by immunoblotting: IgE and IgG antibody-binding studies (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 48 (1993), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The pollen of canary grass, which was introduced as a pasture grass from Europe, is a major allergen in the external environment of southern Australia. Seventeen allergenic fractions of canary grass pollen, ranging in mol. mass from 14 to 100 kDa. have been identified by immunoblotting, using IgE antibodies from sera of 24/30 grass-pollen-allergic subjects. The highest frequency of IgE binding (77%) was to a major 34-kDa fraction (tentatively designated Pha a I). This protein bas been partially purified and identified as a group I allergen by immunodepletion experiments, with partially purified Lol p I (from rye-grass pollen), atopic serum, and Lol p I-specific MAb. In addition, microsequencing of the N-terminus of Pha a I showed an amino acid sequence identical to Lol p I. In a separate study. IgE binding to Western blots of Pha a I, Lol p I. and Cyn d I was investigated in 24 sera and found to occur in 19/24. 18/24, and 9/24. respectively. IgE binding to ail three major allergens, and to both Pha a I and Lol p I, occurred in 8/24 sera. Our findings suggest that while tbe N-terminal sequence of Pha a I is identical to Lol p I, there may be specific allergenic epitopes exclusive to this allergen that are important for allergenicity in southern Australia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1993.tb00728.x
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  • 13
    Electronic Resource
    Electronic Resource
    Components of organic phosphorus in soil extracts that are hydrolysed by phytase and acid phosphatase (2000)
    Springer
    Biology and fertility of soils 32 (2000), S. 279-286 
    Details
    ISSN: 1432-0789
    Keywords: Key words Citric acid ; Organic phosphorus ; Phosphatase ; Phytase ; Soil phytate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Extracts were prepared from soil using water, 50 mM citric acid (pH ∼2.3) or 0.5 M NaHCO3 (pH 8.5), and were incubated with excess phytase from Aspergillus niger to determine the amounts of labile P. Two A. niger phytase preparations were used: (1) a purified form which exhibited a narrow substrate specificity and high specific activity against phytate; and (2) a commercial preparation (Sigma) with activity against a broad range of P compounds. A comparatively large proportion (up to 79%, or 5.7 μg g–1 soil) of the organic P (Po) extracted with citric acid was hydrolysed by the commercial phytase, while between 28% and 40% (up to 3.1 μg g–1 soil) was hydrolysed using purified phytase. By comparison, only small quantities of the Po in water and NaHCO3 soil extracts were enzyme labile. While extractable Po was increased both with increasing concentrations of citric acid (up to 50 mM) and increasing pH (pH 2.3–6.0), enzyme-labile P increased only with citric acid concentration. The labile component of Po in citric acid extracts from soils with contrasting fertiliser histories indicated that enzyme-labile Po is a relatively large soil P pool and is potentially an important source of P for plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740000249
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  • 14
    Electronic Resource
    Electronic Resource
    Intestinal iron absorption and mucosal transferrin in rats subjected to hypoxia (1987)
    Springer
    Annals of hematology 55 (1987), S. 421-431 
    Details
    ISSN: 1432-0584
    Keywords: Intestinal iron absorption ; Hypoxia ; Mucosal transferrin ; Iron binding proteins ; Erythropoiesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Three days hypoxia (0.5 atm) increased the haemoglobin and haematocrit values in rats paralleled by enhanced intestinal iron absorption. The destination of recently-absorbed iron was primarily the erythropoietic system, viz. bone marrow, spleen and red cells. Total plasma transferrin, was increased by 30%, but no significant changes in mucosal transferrin were found. No increase in labelling of mucosal transferrin by absorbed iron was observed. These results suggest that mucosal transferrin does not play a major role in the regulation of intestinal iron absorption in hypoxia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00367458
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  • 15
    Electronic Resource
    Electronic Resource
    Effect of hypoxic exposure on iron absorption in heterozygous hypotransferrinaemic mice (1992)
    Springer
    Annals of hematology 65 (1992), S. 260-264 
    Details
    ISSN: 1432-0584
    Keywords: Iron metabolism ; Mouse strains ; Trans ferrin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Heterozygous hypotransferrinaemic mice show serum iron, haemoglobin and reticulocyte levels similar to those of normal (+/+) controls but the plasma apotransferrin level is significantly reduced. Male heterozygous hypotransferrinaemic Balb/cJ mice and control (+/+) littermates were exposed to 1–3 days' hypoxia at 0.5 atmospheres. Controls increased their haemoglobin and iron absorption significantly during the first day of exposure; serum transferrin levels were increased and iron absorption had returned to normal levels by the third day of exposure. Heterozygotes failed to increase haemoglobin or serum transferrin above normal levels and the elevated iron absorption persisted for longer than in controls. Serum iron levels were lower following hypoxic exposure than in controls. Liver iron loading was significantly enhanced by hypoxia in heterozygotes, but not in controls. These observations demonstrate the importance of the reservoir of plasma apotransferrin in the initial phase of enhanced erythropoiesis, and that the ability to enhance plasma levels of iron-bound transferrin is required in order to increase haemoglobin levels under the hypoxic stress.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01836070
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  • 16
    Electronic Resource
    Electronic Resource
    Nuclear Magnetic Resonance as a Method for Continuously Monitoring Rehydration (1967)
    [s.l.] : Nature Publishing Group
    Nature 216 (1967), S. 474-475 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The uptake of water by several dried materials (for example, macaroni, peas, lentils, collagen fibres) has been followed with an NMR process control instrument (PCI). The experimental method is simple: a known volume of water is added to a weighed dry material in a 100 ml. Nessler tube in the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/216474a0
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  • 17
    Electronic Resource
    Electronic Resource
    A double-blind comparison of felodipine and hydrochlorothiazide added to metoprolol to control hypertension (1988)
    Springer
    European journal of clinical pharmacology 34 (1988), S. 21-24 
    Details
    ISSN: 1432-1041
    Keywords: felodipine ; metoprolol ; hydrochlorothiazide ; hypertension ; blood pressure ; adverse effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary Seventy-six uncomplicated hypertensive patients treated in General Practice, whose seated diastolic blood pressure (Phase V) (dBP) remained ≥95 mmHg after a minimum of 4 weeks treatment with metoprolol 50 mg b.i.d. as antihypertensive monotherapy, were randomized to receive the selective ‘calcium antagonist’ felodipine 5 mg b.i.d. or hydrochlorothiazide 12.5 mg b.i.d. in addition to metroprolol 50 mg b.i.d. The trial duration was 8 weeks, the dose of the felodipine or hydrochlorothiazide being doubled after 4 weeks if ‘control’ of BP (dBP 〈90 mmHg) was not achieved on the initial doses. Over the trial period of 8 weeks, felodipine reduced dBP from 102 to 85 mmHg and hydrochlorothiazide from 101 to 91 mmHg; the dBP reduction in the felodipine group was greater than that in the hydrochlorothiazide group (17 vs 9 mmHg) and the attained dBP lower in the felodipine group. About half of the patients in each group required the higher dose. Both regimes were effective and well tolerated. In the dosages used, felodipine was a slightly more effective antihypertensive drug than hydrochlorothiazide when added to metoprolol. There was no apparent difference in the tolerability of the two regimes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01061411
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  • 18
    Electronic Resource
    Electronic Resource
    Style proteins of a wild tomato (Lycopersicon peruvianum) associated with expression of self-incompatibility (1986)
    Springer
    Planta 169 (1986), S. 184-191 
    Details
    ISSN: 1432-2048
    Keywords: Lycopersicon (self-incompatibility) ; Nicotiana (self-incompatibility) ; Pollination ; S-allele ; Sell-incompatibility ; Style
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The identification, isolation and aminoterminal sequencing of two S-genotype-associated proteins from style extracts of Lycopersicon peruvianum Mill. is reported. There is a high level of homology between these two sequences and with the amino-terminal sequences of other S-allele-associated glycoproteins isolated from Nicotiana alata Link et Otto. These sequences were obtained by a new high-sensitivity method of selected twodimensional gel analysis followed by electroelution and purification of proteins by inverse-gradient high-performance liquid chromatography before sequencing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392312
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  • 19
    Electronic Resource
    Electronic Resource
    Nitrogenase activity of two genotypes of Acacia mangium as affected by phosphorus nutrition (1992)
    Springer
    Plant and soil 144 (1992), S. 51-58 
    Details
    ISSN: 1573-5036
    Keywords: Acacia mangium ; nitrogenase activity ; phosphorus nutrition ; plant genotypes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The specific nodulation, nitrogenase activity (acetylene reduction) and budgets of carbon allocation to respiration by nodulated roots were examined in two provenances of Acacia mangium Willd. grown in a glasshouse for 17 weeks to investigate the effects of soil phosphorus and genotypes of the host plant on symbiotic nitrogen fixation. Application of phosphorus (0–80 mg P kg-1 soil) increased specific nodulation (g nodule dry weight g-1 plant dry weight) of provenance Ma11 by two-fold and the percentage of nodulated root respiration allocated to nitrogenase by 50%, but had no effect on specific activity of nitrogenase or specific respiration coupled with nitrogenase activity. Improved phosphorus nutrition increased the specific nitrogenase activity of provenance Ma9 by 2-fold, the percentage of nodulated root respiration allocated to nitrogenase, and specific nitrogenase-linked respiration by 50%, respectively, but had no effect on the specific nodulation. The percentage of respiration coupled with nitrogenase activity in nodulated root respiration by provenance Ma9 was 60–70% higher than that in provenance Ma11, regardless of phosphorus levels applied. At the optimal level of phosphorus addition (10 mg P kg-1 soil), provenance Ma9 had a lower dry mass than provenance Ma11. This was accompanied by a lower nodulated root respiration and a higher percentage of nodulated root respiration allocated to nitrogenase activity in provenance Ma9.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00018844
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  • 20
    Electronic Resource
    Electronic Resource
    Effects of pH, Ca and Al on the exudation from clover seedlings of compounds that induce the expression of nodulation genes inRhizobium trifolii (1988)
    Springer
    Plant and soil 109 (1988), S. 37-47 
    Details
    ISSN: 1573-5036
    Keywords: β-galactosidase activity ; calcium ; lac fusion ; nod gene expression ; pH ; Rhizobium trifolii ; seedling exudates ; Trifolium repens ; T. subterraneum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The expression of nodulation genes inR. trifolii is induced by flavone compounds present in clover root exudates. In the present experiments a bioassay with an indicator strain ofR. trifolii, which contained thelacZ gene fromEscherichia coli fused to theR. trifolii nodA gene, was used to measure the level ofnod gene expression inR. trifolii. Compounds that stimulatednodA gene expression were shown to be present in exudates of white clover (Trifolium repens L.) and nine cultivars of subterranan clover (T. subterraneum L.) seedling sgrown at a range of pH between pH 3.0 and pH 8.0. Thenod gene-induction activity of exudates was, however, reduced when seedlings of all clover species were grown at pH〉7.0 and at pH〈4.0 and pH〈5.0 for white clover and subterranean clover respectively. No major differences were apparent in the activity of exudates from seedlings of the various cultivars of subterranean clover.Nod gene-induction activity of exudates was shown to increase markedly with seedling age. The presence of Ca at concentrations up to 10 mM in seedling culture solutions also resulted in marked increases in thenod gene-induction activity of seedling exudates. Increases in activity due to the presence of Ca were most apparent at low pH where between 5 and 10-fold increases were observed for white clover and subterranean clover respectively. Conversely, the presence of Al at concentrations up to 60 μM in seedling culture solutions had no effect on thenod gene-induction activity of seedling exudates. The observations that both low pH and Ca concentrations affected thenod gene-induction activity of seedling exudates suggested that the net presence of stimulatory flavones in root exudates was an important contributing factor to the ‘acid-sensitive’ step in nodule formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02197578
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