ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The interaction between lipid peroxidation and phospholipase A2 was investigated in fish muscle microsomes and frozen fish muscle. Lysophosphatidylcholine (LPC), a phospholipid hydrolysis product produced by the activity of phospholipase A2, was detected in the lipids extracted from microsomes peroxidized by incubation with NADH and ADP-Fe3+. No detectable level of LPC was found in the microsomal lipids when NADH was withdrawn from the reaction mixture nor in the untreated microsomal lipids. LPC level in the lipids extracted from unheated microsomes was considerably higher than that from heat-treated microsomes when both were peroxidized by incubation with ascorbate and Fe3+. Phospholipase A2 was activated by either NADH-dependent or ascorbate-induced lipid peroxidation in vitro. The levels of lipid peroxidation in fish muscle were higher when fish were stored at higher frozen storage temperatures and so were the levels of LPC.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1987.tb06596.x
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