ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Of 125 microorganisms that were able to use α-hydroxy acid amides as sole nitrogen source, Ochrobactrum anthropi NCIMB 40321 was selected for its ability to hydrolyse racemic amides l-selectively. The substrate specificity of whole O. anthropi cells is remarkably wide and ranges from α-H-α-amino-, α-alkyl-α-amino, N-hydroxy-α-amino acid amides to α-hydroxy-acid amides. After 50% conversion, both the l-acids formed and the remaining d-amides were present in 〉99% enantiomeric excess, and ammonia accumulated in stoichiometric amounts. Using mandelic acid amide as a model substrate, the hydrolysis was optimized. Optimal rates were observed at pH 8.5 at 50°C. At higher temperatures the initial rate was even higher; however, fairly rapid inactivation occurred.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00192081
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