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Digitale Medien

Sanitization of immobilized biocatalysts for the production of 6-aminopenicillanic acid (2000)

Nováčková, J. ; Plháčková, K. ; Sikyta, B.

Berlin : Wiley-Blackwell

Acta Biotechnologica 20 (2000), S. 161-168

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Details

ISSN: 0138-4988

Schlagwort(e): Life Sciences ; Life Sciences (general)

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Five different chemical reagents and γ-rays were tested for the sanitization of immobilized biocatalysts with high penicillin G acylase (PGA) activity. The most effective chemical reagents were N-cetyl-N,N,N-trimethylammonium bromide (CTAB) and 2-isopropyl-5-methylphenol (thymol). The optimum concentration of CTAB for the treatment of the immobilized enzyme was 0.25% [w/v] and 1 h, for immobilized cells 0. [w/v] and 3 h. The optimum concentration of thymol for the immobilized enzyme was found to be 0.1% [w/v] and 1 h, for immobilized cells 0.27% [w/v] and 2 h. The optimum dose of γ-rays for the sanitization of the immobilized enzyme was established as 3.2 kGy, for immobilized cells as 4.5 kGy.

Zusätzliches Material: 5 Tab.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/abio.370200211

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