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Digitale Medien

A new nucleic acid-protein cross-linking reagent (1977)

Oste, C. ; Parfait, R. ; Bollen, A. ; [weitere]

Springer

Molecular genetics and genomics 152 (1977), S. 253-257

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Details

ISSN: 1617-4623

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Summary A new photoactivable reagent is described, which allows the formation of RNA-protein crosslinks via disulfide bridges in combination with mercaptobutyrimidate. The reconstituted L24 protein-23S RNA complex from the large subunit of E. coli ribosomes has been used as a model system for the cross-linking. The main advantages of the reagent are the absence of U.V. generated cross-links, since photoactivation is carried out at 360 nm, on one hand and the ease of cleavage of the cross-link by mild reduction (β-mercaptoethanol) on the other.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00693078

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