ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract In the absence of added template and primer, DNA synthesis activity which required dATP, dTTP, magnesium ion, and ATP was detected in the cell extracts prepared from a thermophile Bacillus stearothermophilus carrying a plasmid pTB913, but not from the strain without plasmid. Polymer synthesis was detectable only after a lag period and then proceeded at an exponential rate. The DNA synthesized in vitro was the alternating copolymer of dAMP and dTMP, poly(dAT). This reaction was very similar to the de novo DNA synthesis by DNA polymerase I of Escherichia coli, Bacillus subtilis, and Micrococcus luteus, except for the requirement of ATP and thermostability.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1985.tb01579.x
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