ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
A DNA recombination-deficient Rec− mutant of Bacillus stearothermophilus was obtained via mutagenesis from a parental strain B. stearothermophilus MO-3 deficient in neutral protease npr. The Rec− status was confirmed by the fact that no recombinational revertant appeared when a hybrid plasmid pNP13 carrying npr was used. The performance of the temperature-dependent integrative and excisable plasmid pTRA117 was further studied in this Rec− host. Its integration into host chromosome was found to be dependent on Rec, although flanking-homology integration had been disproved. Consequently, the excision product of pTRA117, a thermostable plasmid pTRZ117, did not emerge in the Rec− host.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1987.tb02246.x