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  • 1
    Electronic Resource
    Electronic Resource
    Density Distribution Profiles of T Cells: TM, TG and TA Cells and Response Patterns in Autologous Versus Allogeneic MLRs (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 16 (1982), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Discontinuous Percoll gradients have been used to obtain selected humanperipheral blood T lymphocytes without having to resort to interactions with immune complexes in the fractionation of TM, TG and TA cells. Here, we could show that TA cells represent a heterogeneous population with no distinct density profile, in contrast to light(TG) and heavy (TM) cells. Enriched, heavy TM cells could be shown to be excellent responders in allogeneic MLR while failing to react in autologous MLR In contrast, T cells of light density preferentially respond in autologous compared with allogeneic MLR.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1982.tb00719.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Down-regulation of CD95 (Fas/Apo-1) in the epithelia of adenovirus-infected appendices (1997)
    Oxford, UK : Blackwell Science Ltd
    Histopathology 31 (1997), S. 0 
    Details
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Adenoviral inclusions are commonly seen in appendices from infants with intussusception. They are associated with focal epithelial budding and less frequently with epithelial shedding. These morphological changes could depend on the opposing effects of adenoviral gene products on CD95-mediated apoptosis.〈section xml:id="abs1-2"〉〈title type="main"〉Methods and results:Appendices from intussusceptions with viral inclusions (n = 4) and normal appendices (n = 10) were studied by immunochemistry with anti-adenovirus, anti-CD95 and anti-HLA-DR antibodies. Apoptosis was studied by the TUNEL method. The mucosa of normal appendices contained no adenoviral protein. CD95 was present in all epithelial cells except Paneth cells. HLA-DR was absent in epithelial cells and apoptosis was seen only in germinal centres and in a few surface epithelial cells. The epithelium of appendices from intussusceptions contained nuclear inclusions labelled with anti-adenovirus antibody, always found in the epithelial buds. The epithelial CD95 pattern was drastically altered in adenovirus-infected appendices. CD95 was absent from the budding foci. In these foci, HLA-DR was overexpressed. There was also increased epithelial apoptosis in areas remote from those lacking CD95 antigen.〈section xml:id="abs1-3"〉〈title type="main"〉Conclusions:The appearance of epithelial budding or shedding in appendices from intussusception could be due to focal in situ differences in the expression of adenoviral genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2559.1997.2840875.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Laparoscopic Duhamel procedure (1999)
    Springer
    Surgical endoscopy and other interventional techniques 13 (1999), S. 972-974 
    Details
    ISSN: 1432-2218
    Keywords: Key words: Hirschsprung disease — Aganglionosis — Pull-through — Duhamel operation — Laparoscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: Between February 1995 and June 1998, 30 laparoscopic Duhamel pull-through procedures were performed in our department. Methods: Our main aim was to prove the feasibility of the laparoscopic abdominal Duhamel procedure for different localizations of Hirschsprung disease. We used one camera port and three working ports. The sigmoid colon and posterior rectum were mobilized laparoscopically. A standard posterior colo-anal anastomosis was fashioned and a stapler was used for the anterior anastomosis. The top of the rectum was then closed by endo stapler under laparoscopic vision. Results: Thirty patients underwent laparoscopic surgery for this procedure. Three laparoscopic procedures were converted because of technical difficulties. The operative time was 100–330 mn. Oral feeding was started at a mean postoperative time of 2.5 days. Mean postoperative hospitalization was 9 days. Early postoperative complications included 1 anastomotic leak, 1 retrorectal abscess, 2 urinary infections, and 1 evisceration (after conversion). No enterocolitis or enterocolitis-like symptoms were noted. All patients now have daily spontaneous bowel movements. Conclusion: The laparoscopic Duhamel procedure can be performed safely, giving good results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004649901149
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    Paper (German National Licenses)
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