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  • 1
    Digitale Medien
    Digitale Medien
    ELECTRON MICROSCOPE STUDIES OF KERATINIZATION AS OBSERVED IN GINGIVA AND CHEEK MUCOSA (1960)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 85 (1960), S. 0 
    Details
    ISSN: 1749-6632
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Allgemeine Naturwissenschaft
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1749-6632.1960.tb49966.x
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  • 2
    Digitale Medien
    Digitale Medien
    Superoxide dismutase, catalase, and glutathione peroxidase in the swim bladder of the physoclistous fish, Opsanus tau L (1981)
    Springer
    Cell & tissue research 220 (1981), S. 739-752 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Superoxide dismutase ; Catalase ; Glutathione peroxidase ; Swim bladder ; Opsanus tau
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The antioxidant enzymes superoxide dismutase, glutathione peroxidase, and catalase were measured in the rete mirabile and gas gland epithelium area of the swim bladder of the toadfish Opsanus tau. When the concentration of enzyme in the swim bladder was compared with the concentration in other organs (kidney, heart, gills) of the same fish, the swim bladder was found to have the highest concentration of superoxide dismutase but relatively low levels of glutathione peroxidase and catalase. Cytochemical assay for the peroxidatic activity of catalase confirmed that virtually no catalase is present in epithelial cells of the gas gland. A similar assay for peroxidase revealed a cyanide-sensitive peroxidase in the multilamellar bodies of these cells. Most of the catalase and peroxidase in the rete mirabile appears to be confined to the granules of neutrophils and the cytoplasm of erythrocytes. Enzyme activity in the neutrophils is not inhibited by 10-1 M KCN. Cyanide does appear to inhibit the peroxidase activity in erythrocytes but has little effect on catalase in these cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00210458
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  • 3
    Digitale Medien
    Digitale Medien
    Light-microscopic immunocytochemical localization of fibronectin in the developing rat lung (1983)
    Springer
    Cell & tissue research 233 (1983), S. 113-123 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Lung development ; Fibronectin ; Immunocytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The development of the rat lung is a process of continuing morphological change. Indications from work in other mammalian systems suggest that fibronectin may be important in the control of this process. The present study has examined embryonic, neonatal, and adult lung tissue of the rat by means of the peroxidase-antiperoxidase (PAP) technique to demonstrate fibronectin at the light-microscopic level. Positive reaction was observed with anti-fibronectin serum in all stages examined. Control sections treated with pre-immune serum or no primary serum gave negative results in each case. Fibronectin in adult tissue was localized to the alveolar surface and alveolar basal lamina. Neonatal tissue showed fibronectin on pulmonary tubule walls and in basal lamina while embryonic tissue revealed localization of the protein in the basal lamina and in association with small groups of cells at the base of septal buds. These findings suggest a role for fibronectin in the control of rat lung development. The results are discussed in terms of the known functions of fibronectin as a preliminary matrix for the subsequent deposition of collagenous connective tissue, as a cellular adhesion protein, and as surface-bound material for cellular migration.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00222236
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  • 4
    Digitale Medien
    Digitale Medien
    Ultrastructure of the swim bladder of the goldfish, Carassius auratus (1979)
    Springer
    Cell & tissue research 198 (1979), S. 105-117 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Swim bladder ; Physostome ; Cyprinid ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The swim bladder of the cyprinid Carassius auratus (goldfish) is a two-chambered organ connected to the esophagus by a pneumatic duct. The anterior chamber is lined by a single type of squamous epithelial cell. Two types of epithelial cells are present in the posterior chamber. Flattened cells with differences in the electron density of the cytoplasm line most of the chamber. Darker cells generally contain large amounts of glycogen. Cuboidal epithelial cells also occur in the posterior chamber. A glandular layer external to the muscularis in the posterior chamber is composed of large cells containing little glycogen, an extensive Golgi apparatus, and numerous mitochondria with single large granules. Capillaries and nerves are present in large numbers in this layer. Blood vessels form micro-retia mirabilia in the submuscular layer external to the glandular layer. Vessels are of two distinct types with wide lumina and flattened endothelium characterizing the venous vessels. Arterial vessels have smaller lumina, thick endothelial cells with prominent pinocytotic vesicles, and surrounding pericytes. Collagen is present in three forms in this swim bladder — large tactoids in the tunica externa of the anterior chamber, smaller tactoids in the lamina propria of the posterior chamber, and small fibrils in all other areas.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00234838
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  • 5
    Digitale Medien
    Digitale Medien
    The ultrastructure of the swimbladder of the toadfish, Opsanus tau L. (1975)
    Springer
    Cell & tissue research 164 (1975), S. 85-104 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Swimbladder ; Opsanus tau ; Physoclist ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The anterior chamber of the swimbladder of the toadfish Opsanus tau L. is lined by a single layer of columnar gas gland cells, cuboidal cells that resemble gas gland cells but are located outside of the gas gland region, and squamous cells. Multilamellar bodies are numerous in the gas gland cells and the cuboidal cells and are present in smaller numbers in the squamous cells. Capillaries lie in the lamina propria directly below the epithelial lining. A thick continuous muscularis mucosae and a submucosa consisting of tightly packed cells, cell processes, and connective tissue may contribute to the impermeability to gases of the wall of the anterior chamber. The posterior chamber of the swimbladder is lined by a single type of squamous epithelial cell. Multilamellar bodies were occasionally observed in these cells also. Other types of cells frequently form a partial second layer between the epithelial lining and the basement lamina. A thin muscularis mucosae lies directly below the basement lamina and the capillaries of the posterior chamber are located in the submucosa. The tunica externa is a layer of dense connective tissue that surrounds both the anterior and posterior chambers. Collagen fibrils in the form of tactoids are present in this layer.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00221697
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  • 6
    Digitale Medien
    Digitale Medien
    Ultrastructural immunocytochemical localization of fibronectin in the developing rat lung (1983)
    Springer
    Cell & tissue research 234 (1983), S. 165-177 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Fibronectin ; Lung ; Development ; Ultrastructure ; Immunocytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary In a previous study changes in the macrodistribution of fibronectin during rat-lung development were examined. Using the peroxidase-antiperoxidase immunocytochemical technique, we have demonstrated the presence of fibronectin in embryonic, neonatal, and adult rat lung at the ultrastructural level. In the embryo, fibronectin is found both in an intra-and extracellular association with isolated pneumoblasts, and in a periodic distribution along the basal lamina. The neonate displays fibronectin in an intracellular association with early type-I cells and on their basal and luminal surfaces, but not in association with type-II cells. Neonatal basal lamina is diffusely labeled by anti-fibronectin antiserum. Fibronectin in adult tissue is found both intracellularly and on the basal and luminal surfaces of type-I cells but not in type-II cells. The basal lamina and interstitial connective tissue are slightly or non-reactive. These observations confirm and extend our initial suggestion that fibronectin is involved in rat-lung development.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217410
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  • 7
    Digitale Medien
    Digitale Medien
    Cytochemical study of the lamellar bodies in the swimbladder of the toadfish Opsanus tau L. (1977)
    Springer
    Cell & tissue research 185 (1977), S. 77-87 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Swimbladder (Toadfish) ; Lamellar bodies ; Cytochemistry ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The columnar epithelial cells of the gas gland in the swimbladder of the toadfish, Opsanus tau L., contain lamellar bodies that resemble the lamellar bodies found in epithelial cells of vertebrate lungs. Cytochemical assays indicate that swimbladder lamellar bodies are soluble in chloroform-methanol solution, react with tricomplex flocculation solution (indicating a phospholipid component), exhibit a positive reaction for cholesterol when exposed to digitonin, and contain acid phosphatase. The anterior chamber of the toadfish swimbladder is lined by an extracellular layer. Digitonin-cholesterol crystals are found in this layer when the swimbladder is treated with digitonin. A ruthenium red positive layer is also present in the anterior chamber of the toadfish swimbladder. The structure and cytochemistry of swimbladder lamellar bodies are compared with those of vertebrate lung lamellar bodies. Similarities between the extracellular layer in the swimbladder and the extracellular layer in lungs are also noted.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00226670
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  • 8
    Digitale Medien
    Digitale Medien
    Preliminary observations on the fine structure of oral mucosa (1956)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 126 (1956), S. 225-239 
    Details
    ISSN: 0003-276X
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/ar.1091260208
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  • 9
    Digitale Medien
    Digitale Medien
    The fine structure of granulosa cell nexuses in rat ovarian folliclesThis work is part of a dissertation submitted by the first author to the faculty of arts and sciences, Boston University Department of Biology, in partial fulfillment of the requirements for the Ph.D. degree, 1971. (1973)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 175 (1973), S. 107-125 
    Details
    ISSN: 0003-276X
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: An intercellular junction known as the nexus has been identified in developing granulosa tissue of rat ovaries. Nexuses are common in Graafian follicles of mature cycling rats and in similar follicles of immature animals stimulated by exogenous gonadotrophins. The angle at which the plane of section intersects a nexus significantly governs its internal appearance. Within obliquely cut nexuses there is often a cross-striated pattern of uniform lines in specimens prepared by conventional fixation and staining procedures. These lines could be significant because they may represent points of continuity between communicating cells.Two forms of nexuses are observed. The abutment form is found at cell surfaces, is continuous with plasma membranes, and is frequently associated with cell processes. The other form of nexus is ring-shaped and appears free within the cytoplasm. These circular profiles probably represent transverse views through nexus-girdled cell processes as well as nexus-bound spheres which may have pinched off the processes. Since the profiles from the two sources are ordinarily indistinguishable, both are designated “annular nexuses” even though some may be cell inclusions. Lanthanum tracer can percolate into the central region of abutment nexuses but is absent from the large majority of annular nexuses, suggesting that many of them are isolated from the extracellular space. Reconstruction of serial sections reveals that some annuli are parts of cell inclusions. Sphere formation could be a means of removing nexuses from cell surfaces.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/ar.1091750110
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  • 10
    Digitale Medien
    Digitale Medien
    Ultrastructural changes in hamster lung four hours to twenty-four days after exposure to elastase (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 201 (1981), S. 523-535 
    Details
    ISSN: 0003-276X
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: A single endotracheal instillation of elastase initiates a series of changes in animal lungs that results in a condition resembling human panlobular emphysema. An ultrastructural examination of this series of changes was conducted on the lungs of male golden hamsters exposed to 3H-methylated pancreatic elastase and sacrificed at intervals between 4 hour and 24 days after exposure to enzyme.Lung tissue between 4 and 48 hours showed evidence of hemorrhage and progressive degradation of elastic fibers. Very little indication of epithelial cell damage accompanied these changes. Four days after exposure to elastase, synthesis of new elastic fibers began with the appearance of small clumps of microfibrils in close association with interstitial cells, fibroblasts, and smooth muscle cells. There was also evidence of alterations in alveolar type II cells at this time. Small fibrillar elastic fibers continued to be present in the lung through twenty-four days and may represent a slow repair process or may indicate a structural difference in elastic fibers synthesized after exposure to elastase. Evidence of the continued degradation of elastic fibers could be found up to 16 days after exposure to elastase, revealing that repair processes were occurring in some areas of the lung while destructive processes still predominated in other areas.
    Zusätzliches Material: 26 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/ar.1092010309
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