ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Abstract: We have investigated the postnatal development of cerebellar glutamate decarboxylase (GAD), acetylcholine esterase (AChE), 2′,3′-cyclic nucleotide 3′-phosphohydrolase (CNPase), arylsulfatase, succinate dehydrogenase (SDH), Na,K-ATPase, arylesterase, and lactate dehydrogenase (LDH) activities in homozygous (jj) Gunn rats, comparing them with those in heterozygotes. Also examined were GAD activities in the anterior and posterior parts of the vermis cerebelli on day 13. The specific activities of GAD on the basis of wet weight (g) were lower in jj rats after day 10, and remained unchanged from days 10 to 15 in jj rats with severe hypoplasia. On day 13, GAD on wet weight and protein (mg) bases in the anterior part OfJJ rats was lower than that in the posterior parts of both rats. On the contrary, AChE activities based on wet weight after day 10 and on protein after day 5 in jj rats were significantly elevated. While correlations of GAD on a wet weight basis with the cerebellar wet weight were positive after day 8, those of AChE on wet weight and protein bases were inverse after days 10 and 5, respectively. CNPase and SDH activities based on protein in jj rats were higher after day 15 and showed inverse correlations with the cerebellar wet weight after days 15 and 10, respectively. Arylsulfatase activities on wet weight and protein bases in jj rats, which had a peak on day 20, were significantly high after days 10 and 8, respectively. Arylsulfatase activities by wet weight on days 10 to 20 and by protein after day 8 were inversely correlated with cerebellar wet weight in jj rats. These results suggest that the cerebellar inhibitory neurons, the axons of which make synaptic connections to the target cells from days 10 to 15, are selectively affected by bilirubin inji rats with severe hypoplasia. The enhanced arylsulfatase activity in jj rats on days 10 to 20 may be due to the increased number of lysosomes, suggesting that cell damage by bilirubin followed by cell destruction occurs. A high level of AChE activity in jj rats appears to show a relative increase in density of the mossy fibers in the hypoplastic cerebellum.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1471-4159.1982.tb11499.x
Permalink
