ISSN:
1435-5922
Keywords:
stomach
;
isolated cell
;
cell culture
;
growth factors
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Proliferating cells in the gastric mucosal epithelium were successfully enriched by counterflow elutriation in a medium-sized cell fraction. When inoculated on culture plates coated with E-C-L cell attachment matrix, these cells differentiated into mucus-producing cells after reaching confluence. Northern blot analysis did not detect any transcript of the proton pump, histidine decarboxylase, somatostatin, or pepsinogen I, indicating the absence of parietal, ECL, D, and chief cells in the confluent monolayer. These mucus-producing cell monolayers that respond to various growth factors may be a suitable model with which to investigate the function of gastric mucus cells in vitro.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02348656
Permalink