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  • 1
    Electronic Resource
    Electronic Resource
    Additional reverse transcription–polymerase chain reaction of peripheral slices is not superior to analysis of the central slice in sentinel lymph nodes from melanoma patients (2004)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 150 (2004), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background  The status of the sentinel lymph node (SLN) is an important prognostic factor in patients with cutaneous melanoma. Reverse transcription–polymerase chain reaction (RT–PCR) has been used as a sensitive means of detecting tumour cells in SLNs.Objectives  To determine whether RT–PCR analysis of the SLN using both the central and the peripheral slices is more sensitive than molecular analysis of the central slice only.Methods  Eighty-three SLNs from 59 patients with primary cutaneous melanoma were identified by SLN mapping. All SLNs were bisected along their longitudinal axis to produce two equal halves. One half was used for histology and immunohistochemistry, and the other was analysed by RT–PCR for tyrosinase and MelanA. Parallel to the longitudinal axis, one central slice (approximately 2 mm in thickness) was cut manually. This central slice was used for our standard RT–PCR protocol. In the current study, up to eight additional peripheral slices (each approximately 2 mm in thickness) were cut parallel to the existing cut surface. These peripheral slices were analysed by additional RT–PCR.Results  Standard RT–PCR of the central slice yielded positive results in 34 of 59 patients (57%). Additional RT–PCR of peripheral slices demonstrated positive findings in six additional patients (10%) who were initially negative by standard RT–PCR of the central slice. In detail, seven of those 34 patients positive by standard RT–PCR of the central slice had positive histological findings. In each of these seven patients, RT–PCR was positive both in the central slice as well as in the peripheral slices. The remaining 27 patients with positive RT–PCR results of the central slice showed negative histological findings. Only nine (33%) of these 27 patients had a positive RT–PCR also in the peripheral slices. Finally, all 25 patients with negative RT–PCR results in the central slice showed negative histological findings. Six of these patients (24%) revealed positive RT–PCR results on the analysis of peripheral slices. However, three of these patients expressed only MelanA but not tyrosinase. Thirty lymph nodes from 24 nonmelanoma patients served as negative controls for RT–PCR. In three of these 24 patients (13%) expression of MelanA but not tyrosinase was detected by RT–PCR.Conclusions  Molecular analysis of peripheral slices yielded six additional patients (10%) positive by RT–PCR who were initially negative by standard RT–PCR of the central slice. However, three of these six patients were found to express only MelanA but not tyrosinase. As MelanA expression was also found in 13% of control lymph nodes, positive MelanA expression alone in SLNs of melanoma patients requires cautious interpretation in order to avoid false-positive findings. Thus, additional molecular processing of peripheral slices did not significantly increase the number of patients with RT–PCR-positive SLNs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.2004.05792.x
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  • 2
    Electronic Resource
    Electronic Resource
    ESR-untersuchungen paramagnetisch markierter merrifield-harze
    Amsterdam : Elsevier
    Tetrahedron Letters 13 (1972), S. 3793-3794 
    Details
    ISSN: 0040-4039
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0040-4039(01)94163-2
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  • 3
    Electronic Resource
    Electronic Resource
    Destruction of tumour parenchyma in basal cell carcinoma by tumour-associated neutral proteases: a histochemical study (1991)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 124 (1991), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Proteolytic activity was demonstrated histochemically in frozen sections of basal cell carcinomas (BCCs). After incubation of tissue sections in 0.1 m phosphate buffer with 0.25 m NaCl the tumour epithelium was almost completely destroyed. The basal and squamous cell layers of the disintegrated to varying degrees, particularly where they were directly in contact with tumour epithelium. Serine and metalloprotease inhibitors diminished this tissue destruction, lodoacetate enhanced tumour destruction, urea and potassium thiocyanate even more so. The high proteolytic activity of BCC demonstrated in this study may be an important factor in the proliferative, invasive and destructive behaviour of this tumour.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.1991.tb00572.x
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  • 4
    Electronic Resource
    Electronic Resource
    Clinical risk factors and prognostic significance of local recurrence in cutaneous melanoma (2004)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 151 (2004), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background  True local recurrence (LR) means clinically detectable regrowth of parts of the tumour which were not completely excised. In the literature the term ‘LR’ has been used in a vague and inconsistent manner that may include satellite and in-transit metastasis.Objective  The aim of this study was to establish clinical, histological and surgical risk factors for the manifestation of LR and to evaluate the prognostic significance of LR.Study design  Data from 3960 Stage I and II melanoma patients who visited the melanoma clinic of the Department of Dermatology at the University of Tuebingen from 1980 to 1999 were documented in a prospective manner. A retrospective comparative analysis of patients with and without LR was performed.Results  Of all patients 1·4% had a LR as a first recurrence and 1·7% had a LR in the course of the follow-up period. LR were most frequent after previous clinical or histological misdiagnosis and inadequate therapy. In the univariate analysis significant risk factors for LR-free survival were age, tumour surface area, locality, tumour thickness, level of invasion, histological type, associated naevus, surgery (one step vs. multiple steps) and compliance with recommended excision margins. In the multivarate analysis the factors locality (P 〈 0·0001), tumour thickness (P = 0·0086) and compliance with recommendations on excision margins (P = 0·014) were significant independent risk factors for the manifestation of LR. The overall survival of patients with LR as first progression did not significantly differ from the overall survival of the other patients with melanoma (P = 0·60).Conclusion  True LR is a rare event for which tumour locality, tumour thickness and surgery are independent risk factors. The occurrence of LR might not impair the prognosis of melanoma patients. However, in the published literature numerous definitions of ‘LR’, including lymphogenic metastasis, complicate comparison.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.2004.06050.x
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  • 5
    Electronic Resource
    Electronic Resource
    Detection of melanoma cells in sentinel lymph nodes, bone marrow and peripheral blood by a reverse transcription–polymerase chain reaction assay in patients with primary cutaneous melanoma: association with Breslow's tumour thickness (2001)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 145 (2001), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Tyrosinase reverse transcription–polymerase chain reaction (RT–PCR) has been shown to be highly sensitive in detecting tumour cells in melanoma patients. Objective To assess whether the detection of minimal residual disease by RT–PCR is improved by concomitant analysis of sentinel lymph nodes (SLNs), bone marrow (BM) and peripheral blood (PB) in patients with primary melanoma. Methods Thirty-five SLNs, 41 BM samples and 26 PB specimens from 26 patients with primary cutaneous melanoma (tumour thickness ≥ 0·75 mm) were examined by nested RT–PCR for tyrosinase and Melan-A. SLNs and BM samples were also analysed by histopathology. RT–PCR findings were related to tumour thickness of the primary melanoma. Results Overall, melanoma cells were detected by RT–PCR in 13 of 26 patients (50%). Seven patients had positive RT–PCR results in their SLNs (27%), including all patients (n = 4) with histologically positive SLNs, two patients had positive findings in their BM exclusively detected by RT–PCR (8%) and six patients in PB (23%). The presence of tumour cells detected by RT–PCR in SLNs was not related to the presence of melanoma cells in BM and/or PB. The incidence of RT–PCR-positive SLNs was significantly associated with greater tumour thickness (P = 0·004). Both patients with positive RT–PCR findings in their BM had a large tumour thickness (≥ 2 mm). No association between positive RT–PCR findings in PB and greater tumour thickness was observed. Conclusions RT–PCR-positive SLNs were strongly associated with greater tumour thickness, underlining the prognostic significance of SLN positivity. Similar to certain epithelial malignancies, molecular investigation of the BM might provide complementary prognostic information in the early stages of melanoma. In contrast, no association between positive RT–PCR results in PB and increasing tumour thickness was found, implying that RT–PCR findings in PB are of doubtful clinical relevance in primary melanoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.2001.04334.x
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  • 6
    Electronic Resource
    Electronic Resource
    Unblutige Blutdruckmessung an Kleintieren mit dem Kondensatormikrophon nach Brecht und Boucke (1955)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 225 (1955), S. 190-190 
    Details
    ISSN: 1432-1912
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00246858
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  • 7
    Electronic Resource
    Electronic Resource
    Eine histologische Aufarbeitungstechnik von Hauttumorexzisaten zur lückenlosen Schnittrandkontrolle (1996)
    Springer
    Der Pathologe 17 (1996), S. 127-129 
    Details
    ISSN: 1432-1963
    Keywords: Schlüsselwörter Maligne Hauttumoren ; Basaliom ; Plattenepithelkarzinom der Haut ; Lentigo maligna ; Lentigo-maligna-Melanom ; Dermatofibrosarcoma protuberans ; Mikrographische Chirurgie ; Key words Malignant skin tumors ; Micrographic surgery ; Basal cell carcinoma ; Squamous cell carcinoma ; Lentigo maligna ; Lentigo maligna melanoma ; Dermatofibrosarcoma protuberans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary In the treatment of basal cell carcinoma, squamous cell carcinoma and a number of other tumors of the skin, the reccurrence rate is tenfold lower if they are treated with micrographic surgery in comparison with tumors, assessed by parallel histological sections. We demonstrated this in our investigations including over 5900 cases. The rate of local recurrences is under 1 %. The difference can be explained by the typical growth pattern of skin tumors. In the following article two simple methods of tissue processing are described, which can be used for formalin-fixed tissues and for fresh tissues.
    Notes: Zusammenfassung Bei der Behandlung von Basaliomen, Plattenepithelkarzinomen sowie einer Reihe weiterer Tumoren der Haut zeigen Fälle, die mittels einer dreidimensionalen, lückenlosen histologischen Aufarbeitung der Schnittränder untersucht und bis zum Nachweis tumorfreier Ränder nachoperiert wurden (mikrographische Chirurgie), eine um eine Zehnerpotenz geringere Rezidivrate wie Patienten, deren Tumorexzisate mit parallelen Serienschnitten beurteilt wurden. Dies konnte auch in unseren Untersuchungen an über 5900 Fällen demonstriert werden. Die Rezidivrate liegt dabei unter 1 %. Der Unterschied läßt sich durch das typische subklinische Wachstumsverhalten von epithelialen Hauttumoren erklären. In der folgenden Arbeit werden 2 einfache Methoden der technischen Aufarbeitung beschrieben, welche auch für Einsendehistologien gut geeignet sind.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002920050144
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  • 8
    Electronic Resource
    Electronic Resource
    Über Blutdruckreaktionen auf künstliche Hypo- und Hyperthermie und ihre pharmakologische Beeinflußbarkeit (1955)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 225 (1955), S. 251-259 
    Details
    ISSN: 1432-1912
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Zusammenfassung 1. An Ratte, Meerschweinchen und Goldhamster wurden die Reaktionen des Blutdrucks auf rasche Auskühlung und Erwärmung und deren Beeinflußbarkeit untersucht. 2. Die Blutdrucksteigerung bei Auskühlung und bei Überwärmung ist eine zentral bedingte Notfallfunktion. 3. Die gefundenen Blutdruckbewegungen wurden unterdrückt a) durch zentrale Hemmung: völlig von Narkotica (tiefste Narkose), Dolantin, Pyrazolonen, Megaphen und Novocainamid; teilweise von den Antihistaminica der Phenothiazinreihe, Dromoran und Cliradon. b) durch periphere Hemmung: von allen Adrenolytica, darunter auch Megaphen und Spartein. c) durch Blockade des Nebennierenmarkes: von Spartein. 4. Ohne Hemmwirkung waren Morphin, Ganglienblocker und Parasympathicolytica. 5. Zwischen Hemmwirkung auf die untersuchten Blutdruckreaktionen, analgetischer und antipyretischer Wirkungsstärke lassen sich keine deutlichen Beziehungen feststellen, vielmehr scheinen die Angriffspunkte der verschiedenen zentral wirksamen Pharmaka bezüglich der Hemmung der Notfallfunktion sehr different zu sein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00247776
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  • 9
    Electronic Resource
    Electronic Resource
    Qualitätssicherung in der operativen und onkologischen Dermatologie Stellungnahme der VOD zur Therapie erworbener Nävuszellnävi (1997)
    Springer
    Der Hautarzt 48 (1997), S. 705-706 
    Details
    ISSN: 1432-1173
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001050050647
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  • 10
    Electronic Resource
    Electronic Resource
    Shave-Exzision von melanozytären Nävi der Haut Indikation, Technik, Ergebnisse (2000)
    Springer
    Der Hautarzt 51 (2000), S. 575-580 
    Details
    ISSN: 1432-1173
    Keywords: Schlüsselwörter Shave-Exzision ; Nävi ; Rezidive ; Kosmetische Ergebnisse ; Frühmelanome ; Keywords Shave excision ; Nevi ; Recurrences ; Cosmetic results ; Early melanoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Abstract Background and objective. Shave excision of nevi is a technique still under debate. Speed, simplicity, and the fact that it provides excised material for histologic examination are contrasted with the lack of excision margins and a higher rate of nevus recurrences. In this study, the pros and cons of shave excision were evaluated. Patients and methods. Conventional excisions (268 nevi with intracutaneous butterfly sutures) and shave excisions (403 nevi) were compared with the patients' subjective assessments and objective parameters as recurrence, color, depth, surface smoothness of the scars, and the healing process. The nevi, found on the entire integument, ranged in diameter from 2 to 15 mm, with an average of 5 mm. A second excision was performed only in cases in which an early malignant melanoma could not be excluded. Results. Shave excisions were evaluated subjectively as being better. Shave excisions resulted in fewer complications (7.9% versus 15%), but recurrences were more frequent (18.1% versus 6.0%). There was no close realtionship between histopathologic finding of complete excision and recurrences. Conclusions. Small nevi without clinical suspicion of malignant melanoma can be removed with the shave excision technique with good results. Patients should be informed about the higher rate of recurrences. The appliance of the shave technique requires exact knowledge and experience, enabling good histopathologic examinations.
    Notes: Zusammenfassung Hintergrund und Fragestellung. Die Shave-Exzision von melanozytären Nävi ist umstritten. Der Schnelligkeit und Einfachheit des Eingriffs mit der Möglichkeit der histologischen Untersuchung des Exzisats stehen die oft knappe Entfernung und häufigere Nävusrezidive gegenüber. Hier werden Vor- und Nachteile gegeneinander abgewogen. Material und Methode. Konventionelle Exzisionen mit Wundverschluss durch intrakutane Schmetterlingsnähte (n=268) und Shave-Exzisionen (n=403) wurden verglichen durch die subjektive Beurteilung der Patienten und objektive Parameter wie Rezidiv, Farbe und Oberfächenbeschaffenheit der Narben. Die Nävi hatten einen Durchmesser von 2–15 mm, im Median 5 mm, und befanden sich am gesamten Integument. Eine Nachexzision erfolgte nur, wenn ein Frühmelanom nicht sicher auszuschließen war. Ergebnisse. Die Patienten bewerteten die Shave-Exzisionen etwas besser. Sie zeigten seltener Komplikationen (7,9% vs. 15%), aber häufiger Rezidive (18,1% vs. 6,0%). Es bestand keine enge Beziehung zwischen den histologischen Befunden inkompletter Exzision und Rezidiven. Schlussfolgerung. Kleinere Nävi sind recht gut durch Shave-Exzisionen zu entfernen, wenn kein Melanomverdacht besteht. Die höhere Rezidivrate muss dem Patienten mitgeteilt werden. Die Technik verlangt eingehende Kenntnis des Vorgehens und Erfahrung, damit gute histologische Untersuchungen gewährleistet sind.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001050051174
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