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  • 1
    Electronic Resource
    Electronic Resource
    Silencing of a β-1,3-glucanase transgene is overcome during seed formation (1997)
    Springer
    Plant molecular biology 34 (1997), S. 125-137 
    Details
    ISSN: 1573-5028
    Keywords: developmental regulation ; Β-1,3-glucanases ; transgene silencing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Expression of a β-1,3-glucanase transgene (gn1) driven by the CaMV 35S promoter is silenced in the T17 homozygous tobacco transgenic line. This silencing process is post-transcriptionally regulated and subject to developmental control. We have examined this phenomenon to investigate the developmental pathways involved in suppression and reactivation of gn1 expression as well as to identify the plant tissues where these processes occur. Analysis of β-1,3-glucanase activity and gene expression have allowed us to determine that suppression of gn1 is a very efficient process reducing the steady-state gn1 mRNA level, simultaneously, in all leaves of the plant. Gene silencing occurs a few weeks after seed germination, and is maintained throughout vegetative growth and floral development. Expression of gn1 is restored in the maturing fruit some time after fertilization. In situ hybridization analyses show that expression of gn1 is restored within the developing seeds in tissues derived from meiotically divided cells. In contrast to the high level of expression found in seedlings obtained from germinated T17 homozygous seeds, the expression of gn1 is not reactivated in plantlets regenerated in vitro from leaf explants of suppressed T17 homozygous plants that is, in plant tissues obtained by mitotic division. Thus, reactivation of gn1 expression specifically occurs along the developmental programme controlling sexual reproduction and likely throughout epigenetic modifications affecting the state of gene expression during meiosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005882106266
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Structure and induction pattern of a novel proteinase inhibitor class II gene of tobacco (1995)
    Springer
    Plant molecular biology 27 (1995), S. 1197-1204 
    Details
    ISSN: 1573-5028
    Keywords: pathogenesis-related proteins ; proteinase inhibitor ; signal transduction ; wounding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA and a corresponding genomic clone encoding a protein with partial identity to type II proteinase inhibitors from potato, tomato and Nicotiana alata, were isolated from tobacco libraries. The protein of 197 amino acids contains a putative signal peptide of 24 residues and three homologous domains, each with a different reactive site. The tobacco PI-II gene is not expressed in leaves of healthy plants, but is locally induced in leaves subjected to different types of stress (TMV infection, wounding, UV irradiation) and upon ethephon treatment. As opposed to the analogous PI-II genes of potato and tomato, the tobacco gene is not systemically induced by wounding or pathogenic infection. A far-upstream region in the PI-II promoter, containing various direct and indirect repeats, shares considerable sequence similarity to a similar region in the stress-inducible Cu/Zn-superoxide dismutase gene of N. plumbaginifolia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020893
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    Paper (German National Licenses)
    Fulltext
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