ISSN:
1572-8854
Keywords:
Nucleic acid–water interaction
;
water mediated recognition in biology
;
inosine 5′-monophosphate polyhydrated complex
;
variant and invariant water molecules for nucleotide recognition
Source:
Springer Online Journal Archives 1860-2000
Topics:
Geosciences
,
Physics
Notes:
Abstract The crystal structure of an octadecahydrated complex between two inosine 5′-monophosphate (IMP) has been determined at atomic resolution, which reveals the hydrogen bonding and the coordination cooperativity of water molecules to nucleotide recognition. The crystal belongs to monoclinic space group P21 with cell dimensions a = 8.65(1), b = 21.90(1), c = 12.37(1)Å, and β = 110.38(9)°. The ribose hydroxyls, purine N7, keto(O6) bonded water molecules W1, W2, W5, W6, W8 and the phosphate bridge forming water oxygens of W4, W7, W11 appear to play an invariant role in their hydrogen bonding interactions with the IMPs. The synergistic role of the water molecules W5, W6, W8 in the purine staking domain N27⋯W5=2.583,O16⋯W8=2.759,O2627⋯W6=2.723 Åhave been clearly observed for the first time. The complexation of the water molecules through variable hydrogen bonding coordination indicate their functional involvement through extensive cooperative donor-acceptor network mechanism. The occurrence of hydrogen-bonded water spines, water bridges and their interplay in the structural association of IMPs could indicate the possible viability of those aquatic centers in the biological situation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1023235820633
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