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  • 1
    Electronic Resource
    Electronic Resource
    Book reviews (1975)
    Springer
    Plant ecology 30 (1975), S. 73-76 
    Details
    ISSN: 1573-5052
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02387881
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  • 2
    Electronic Resource
    Electronic Resource
    Role of ethylene in auxin-induced flower bud formation in tobacco explants (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 78 (1990), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effect of ethytene on in vitro flower bud formation in thin-layer explants from tobacco pendicels (Nicotiana tabacum L. cv. Samsun) was studied Endogenous ethylene production was stimulated by l-minocyclopropanc-l-carhoxylic acid (ACT), and inhibited by aminoethoxyviny lglycine (AVG). resulting in higher and lower ethylene accumulation. respectively. In the presence of an elevated ethylene concentration, the number of flower buds formed after 7 days of culture in explants was increased, compared with the control. Treatment with AVG or with AgNO3 which blocks ethylene action resulted in decreased bud numbers after 7 days of culture. A different effect of ethylene was visible after 14 days of culture, when regeneration was complete. Treatment with AgNO3 led to more bud regeneration, and increasing ethylene concentrations to lower bud numbers. The endogenous production of ethylene was enhanced by high concentrations of 1-naphthaleneacetic acid (NAA).The inhibitory effect of applied ethylene was almost 100% in explants cultured at low concentrations of NAA (below 1 μM). but hardly visible at high concentrations (4.5 μM). As a consequence, the optimal NAA concentration shifted to a higher value in the presence of ethylene. These results are interpreted as a reduction in tissue sensitivity to auxin and in regenerative capability by ethylene. The effect of ethylene on auxin action is not exerted at the level of hormone concentration. Neither NAA uptake nor conversion to conjugates was effected by ethylene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1990.tb02076.x
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  • 3
    Electronic Resource
    Electronic Resource
    Development of flower buds in thin-layer cultures of floral stalk tissue from tobacco: Role of hormones in different stages (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 61 (1984), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The in vitro development of flower buds was studied on tissue explants of epidermis and subepidermal cortex from the flower stalks of Nicotiana tabacum L. cv. Samsun. The number of flower buds formed depended mainly on cytokinin concentration. Auxin acted as a modifier in a complex way. In early development, NAA at 1 μM decreased the number of buds initiated and delayed bud emergence. At a later stage, auxin promoted bud outgrowth at the same concentration. Optimal results were obtained when explants were first incubated at low auxin concentration for 3–5 days and subsequently transferred to an elevated auxin level. Physiological processes that lead to flower bud initiation start very soon after the onset of incubation. This was inferred from experiments whereby explants were first cultured at an inductive cytokinin concentration and then transferred to a non-inductive hormone level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1984.tb06109.x
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  • 4
    Electronic Resource
    Electronic Resource
    Uptake and metabolism of benzyladenine in the early stage of flower bud development in vitro in tobacco (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 74 (1988), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Benzyladenine (BA) was found to regulate the number of flower buds regenerated in vitro from pedicel tissue of tobacco. Flower bud induction was particularly sensitive to BA levels in the range of 0.45 to 1.0 μM, where a two-fold increase in concentration caused a threefold rise in the number of buds. When tissues were fed radioactive BA for 24h, only 9–12% of the counts were recovered in the original compound. The rest was present in metabolites, tentatively identified as the mono-, di- and triribotides, 7- and 9-glucosides and 9-riboside of BA. The amount of growth regulator taken up and the quantities of BA and its metabolites in the explants were all linearly related to the concentration of the medium. The internal BA concentration was ca 60% of the level in the medium after 24 h. When the concentration in the medium was raised, relatively more BA remained in the non-conjugated form. However, this change in the equilibrium between BA and the conjugates is too small to account for the steep rise in the curve representing concentration vs effect between 0.45 and 1.0 μM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1988.tb04950.x
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  • 5
    Electronic Resource
    Electronic Resource
    Floral morphogenesis in thin-layer tissue cultures of Nicotiana tabacum (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 62 (1984), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The morphological changes in thin-layer tissues of Nicotiana labacum L. cv. Samsun, cultured on Murashige and Skoog medium with 1 μ M each of naphthalene acetic acid (NAA) and benzyladenine (BA), were studied during the first 8 days of culture with light and scanning electron microscopy. The first three days of culture arc characterized by enlargement of all cells and cell divisions starling in the cortical parenchyma cells adjacent to the medium. Between days 3 and 6, epidermal and/or subepidermal cells start to divide, resulting in division centers, which lead to flower bud formation. The hormones NAA and BA in different concentrations affect the formation and distribution of flower buds, bud morphology and callus formation. BA influences particularly bud formation and bud morphology, while NAA affects callus formation in particular. In addition, polarity may occur in the formation of both callus and flower buds, the degree of which depends upon the hormone concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1984.tb05927.x
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  • 6
    Electronic Resource
    Electronic Resource
    Expression of two gibberellin-regulated cDNAs during early flower development in tomato (Solanum lycopersicon). Effect of grafting and paclobutrazol (2000)
    Copenhagen : Munksgaard International Publishers
    Physiologia plantarum 108 (2000), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: To study the role of translocation of gibberellin (GA) intermediates or bioactive GAs from other plant parts to buds during early flower development in tomato (Solanum lycopersicon), the effect of grafting and paclobutrazol (PAC) treatment on the expression of tgas100 and tgas118, two GA-regulated mRNAs, was analysed. Both mRNAs accumulated in a dose-dependent fashion. Application of 0.5 ng GA3 per bud to developmentally arrested flower buds of a GA-deficient mutant of tomato (gib-1) induced tgas100 expression, while the tgas118 abundance increased. For obtaining normal flower development through anthesis in the mutant, a single GA3 treatment was required of at least 5 ng GA3 per bud. In wild-type flower buds, PAC decreased the abundance of tgas100 and tgas118 mRNAs either when PAC was sprayed on whole plants or directly applied to buds. When only the wild-type buds were treated with PAC, the expression profiles characteristic for untreated buds were not restored by translocation of endogenous GAs. Grafting of gib-1 scions onto wild-type donor plants did not result in normal flower development or expression profiles like in wild-type buds. We conclude that the role of GA transport in early flower development of tomato is negligible and that the GAs required for development have to be synthesized in the flower bud itself.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1399-3054.2000.108001095.x
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  • 7
    Electronic Resource
    Electronic Resource
    The role of endogenous gibberellins during fruit and seed development: Studies on gibberellin-deficient genotypes of Arabidopsis thaliana (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 67 (1986), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Siliqua development was studied in the wild type line Landsberg erecta and the GA-sensitive mutant ga-1 of Arabidopsis thaliana (L.) Heynh. Reciprocal crosses between wild type and ga-l mutant, and self pollinations of either parent have shown that siliqua growth is determined by endogenous GAs originating from maternal tissues and embryo. The ga-1 mutant either self pollinated or cross-pollinated with wild type pollen showed reduced siliqua growth, which to a large extent could be overcome by exogenously applied GAs. The siliquae of the ga-1 mutant possessed very reduced ent-kaurene synthesizing capacity and no detectable endogenous GA-activity indicating an early block in the GA-biosynthetic pathway. Seed weight is not affected by GA-deficiency during development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1986.tb02463.x
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  • 8
    Electronic Resource
    Electronic Resource
    Formation of bound gibberellins inPharbitis nil (1971)
    Springer
    Planta 99 (1971), S. 290-301 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Developing seeds ofPharbitis nil accumulate free as well as bound gibberellins until a maximum level is reached at approximately 25 days after anthesis. Seeds from CCC-treated parent plants have a strongly reduced level of free as well as bound gibberellins. When different spray reagents were used it was found that trichloroacetic acid in particular was suitable to locate non-hydrolysed bound GA fractions on thin-layer plates. Chromatography showed two major bound GA fractions, determined with spray reagents as well as by means of hydrolysis.3H-GA1 applied to youngPharbitis plants was converted to two water-soluble compounds present in the aqueous phase. The rate of conversion was significantly enhanced when3H-GA1 and14C-glucose were applied to the same plants. Chromatography indicated that one of the conversion products of3H-GA1 became at least partly associated with the applied14C-glucose (or its products). This suggestion was also supported by the fact that mild acid hydrolysis of the aqueous fraction resulted in the reappearance of3H-GA1 and a conversion product of3H-GA1, including a14C-radioactivity peak cochromatographing with14C-glucose. However, the conversion products obtained with3H-GA1 applied to plants appeared to be chromatographycally different from any of the bound-GA fraction established by means of hydrolysis or spray reagents in developing seeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385821
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  • 9
    Electronic Resource
    Electronic Resource
    The metabolism of applied gibberellic acid in Pharbitis nil choisy: tentative identification of its sole metabolite as gibberellic acid glucoside and some of its properties (1975)
    Springer
    Planta 126 (1975), S. 25-35 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Radioactive gibberellic acid ([14C]GA3) applied to seedlings of Pharbitis nil strain Violet is converted to one single metabolite (R-[14C]GA3), which has been tentatively identified as GA3-glucoside. As with authentic GA3-glucoside, R-[14C]GA3 can be hydrolysed to some extent with cellulase and β-glucuronidase, but hardly at all with β-glucosidase. Acid hydrolysis, which is more effective than enzymatic hydrolysis, yielded GA3 as well as a biological inactive compound. The latter represents a degradation product of GA3 due to the sensitivity of GA3 to acidic conditions. The R-[14C]GA3, like authentic GA3-glucoside, possesses little or no biological activity. R-[14C]GA3 applied to developing seeds is partly hydrolysed during imbibition of the mature seed but is, however, reconverted to R-[14C]GA3 during subsequent germination. Applied R-[14C]GA3 is strongly accumulated in the cotyledons of Pharbitis seedlings, to a greater extent than [14C]GA3. However, unlike [14C]GA3 it is not accumulated in the apical regions of the hypocotyl. Furthermore no competition was observed between R-[14C]GA3 and [14C]GA3, which suggests that they do not compete for the same sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00389357
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  • 10
    Electronic Resource
    Electronic Resource
    Book reviews (1983)
    Springer
    Theoretical and applied genetics 65 (1983), S. 212-212 
    Details
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00308068
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