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  • 1
    Electronic Resource
    Electronic Resource
    Rapid-cycle PCR detection of Pyrenophora graminea from barley seed (2001)
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 50 (2001), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Sixty RAPD primers were used to screen for a diagnostic marker that could be used to identify Pyrenophora graminea, a fungal seedborne pathogen that causes leaf stripe on barley. Primer pairs were designed to differentiate P. graminea from other Pyrenophora spp. using a sequence-characterized amplified region (SCAR) approach. A pair of P. graminea-specific primers (PG2 F/R) was obtained that amplified a single fragment from 37 isolates of P. graminea tested, but not from 29 isolates of other Pyrenophora spp. or 12 saprophytes isolated from barley seed. Rapid PCR detection was achieved using a LightCycler, in which the emission of fluorescence from the binding of SYBR Green I dye to the PCR products is measured. The P. graminea-specific product resulting from amplification with PG2 F/R can be distinguished from any nonspecific products by post-PCR melting point analysis. The PCR assay involves 40 amplification cycles of PCR, and the total PCR test including melting point analysis takes 25 min to complete. The rapidity of this test, combined with the closed ‘in-tube’ detection of PCR products, which reduces the potential for contamination, offers significant advantages compared with conventional laboratory and PCR analyses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3059.2001.00563.x
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  • 2
    Electronic Resource
    Electronic Resource
    Quantitative genetic analysis of growth in larval scallops (Placopecten magellanicus) (1996)
    Springer
    Marine biology 124 (1996), S. 671-677 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We estimated the broad-sense heritability of larval size in 20 full-sib families of the giant scallop, Placopecten magellanicus (Gmelin, 1791) grown in laboratory culture in August and September 1991. The goal was to compare scallops with other bivalves which have been shown to have significant heritabilities for larval growth. Secondly, we estimated the lipid content of occytes from female parents, since this is hypothesized to affect larval growth and survival. Finally, we estimated the among-family variation in mortality from 4 to 21 d as a test of possible genetic variation for viability among larval scallops. Significant genetic variation (h2=1.10 to 1.24) was estimated for larval shell length at 4, 14, and 21 d. There was a significant correlation (r=0.66) between larval size at 4 d and lipid content of oocytes, but only when two females with high levels of lipid oocyte−1 were excluded as outliers. There was no correlation between larval size at 14 and 21 d and lipid content of oocytes. Mortality among families from 4 to 21 d was high (69 to 97%), and was significantly different among families. These results indicate that there is significant heritability for larval growth which is largely independent of the lipid content of the oocytes. A high heritability for larval growth may indicate that this trait is only weakly correlated with fitness.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00351048
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic variation among populations of Mytilus spp. in eastern Newfoundland (1995)
    Springer
    Marine biology 124 (1995), S. 417-424 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Limited sampling of Mytilus spp. in eastern Newfoundland has suggested that M. trossulus Gould and M. edulis L. are distributed as pure populations or mixtures of both species. In 1989 and 1990 we collected a wider series of samples of Mytilus spp. from 16 sites in eastern Newfoundland in order to determine the genetic structure based on four polymorphic enzyme loci (Est, Pgm, Lap, Ap). Associations among loci (particularly Pgm and Est), and a bimodal distribution of individual principal-component scores (based on fourlocus genotypes) provided evidence for the presence of both M. trossulus and M. edulis. Most of the sites consisted of a mixture of the two species. The relative frequency of each species in the samples varied on both a macro- and microgeographic scale. Two wave-exposed sites had the highest frequency (〉90%) of M. trossulus. The distribution of hybrid index scores, based on three partially diagnostic loci (Est, Pgm, Lap), provided no evidence for hybridization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00363915
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  • 4
    Electronic Resource
    Electronic Resource
    Morphological variation of Mytilus edulis and Mytilus trossulus in eastern Newfoundland (1999)
    Springer
    Marine biology 133 (1999), S. 691-699 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Allopatric populations of Mytilus species show distinct shell morphology which may be due to genetic and/or environmental effects. Sympatric populations of Mytilus species show similar shell morphology which may be due to hybridization eroding morphological differences and/or the influence of common environmental conditions. The present study examined shell morphology and shell shape from 16 sites in eastern Newfoundland where M. edulis L. and M. trossulus Gould coexist in common environments with limited hybridization. Shell morphology was based on measurements of eight characters, and shell shape was quantified by elliptic Fourier analysis of shell outlines. Significant differences were observed between species for both shell morphology and shell shape across 16 sites sampled. The relatively small differences in morphology and shape between the species were probably due to exposure to common environments rather than hybridization. Shell shape for M. edulis was more eccentric compared to M. trossulus which was more elongated. Shell shape analysis of a range of size classes at one site showed a change from an eccentric to an elongated shape going from the smaller to the larger size classes. Both species showed a similar trend, with the larger M. edulis more eccentric and the larger M. trossulus more elongated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002270050510
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    Paper (German National Licenses)
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