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  • 1
    Electronic Resource
    Electronic Resource
    The effect of temperature on colony growth by Erysiphe sp. infecting Rhododendron (1998)
    Oxford, U.K. and Cambridge, USA : Blackwell Science Ltd
    Plant pathology 47 (1998), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The growth and reproduction of powdery mildew pathogens is generally encouraged by increasing temperatures, up to 25°C. Germination and germ tube extension of Erysiphe sp. on Rhododendron cv. Elizabeth were optimal at 20°C for conidia originally formed at either 10 or 15°C. During a 50-day period of colony growth, the viability of conidia formed at 15°C declined but for those formed at 10°C it increased. The expansion of Erysiphe colonies over an initial 12-day period was favoured by incubation at 15°C compared with 10 or 20°C. In the first 8 to 10 days of growth, secondary and tertiary hyphae formed most rapidly at 20°C. After 12 days, expansion of colonies at 20°C was limited to the area initially infested by primary hyphae, whereas in colonies grown at 10 and 15°C secondary and tertiary hyphae had extended beyond the area first colonized. Small colonies of densely packed hyphae formed at 20°C compared with open spreading colonies observed at 10 and 15°C.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3059.1998.00252.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Rapid-cycle PCR detection of Pyrenophora graminea from barley seed (2001)
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 50 (2001), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Sixty RAPD primers were used to screen for a diagnostic marker that could be used to identify Pyrenophora graminea, a fungal seedborne pathogen that causes leaf stripe on barley. Primer pairs were designed to differentiate P. graminea from other Pyrenophora spp. using a sequence-characterized amplified region (SCAR) approach. A pair of P. graminea-specific primers (PG2 F/R) was obtained that amplified a single fragment from 37 isolates of P. graminea tested, but not from 29 isolates of other Pyrenophora spp. or 12 saprophytes isolated from barley seed. Rapid PCR detection was achieved using a LightCycler, in which the emission of fluorescence from the binding of SYBR Green I dye to the PCR products is measured. The P. graminea-specific product resulting from amplification with PG2 F/R can be distinguished from any nonspecific products by post-PCR melting point analysis. The PCR assay involves 40 amplification cycles of PCR, and the total PCR test including melting point analysis takes 25 min to complete. The rapidity of this test, combined with the closed ‘in-tube’ detection of PCR products, which reduces the potential for contamination, offers significant advantages compared with conventional laboratory and PCR analyses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3059.2001.00563.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The repression and stimulation of growth of Erysiphe sp. on Rhododendron by fungicidal compounds (1997)
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 46 (1997), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Erysiphe sp. is a causal agent of powdery mildew on Rhododendron. A novel in vivo method permitting the screening of fungicides on woody plants is described. Eight fungicides were evaluated for activity against Erysiphe sp. using Rhododendron ponticum microplantlets grown in vitro. Pathogen development changed with both the type of fungicidal compound and the concentrations applied. The most active materials were fenpropidin and penconazole, which showed high activity at the lowest concentrations. Six of the compounds performed more effectively than a mixture of bupirimate + triforine (Nimrod T), the standard recommendation for control of this pathogen on Rhododendron. All fungicides affected the sporulation of Erysiphe sp., with propiconazole, pyrazophos and triadimenol causing a significant increase in sporulation at the lowest concentrations. At higher concentrations, sporulation was significantly reduced by all treatments. No phytotoxic effects were detected with any fungicide at any concentration. The growth of plantlets in most treatments showed no significant difference from the untreated controls. The results of the study are discussed in relation to strategies for control and the epidemiology of Erysiphe sp. infecting Rhododendron.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3059.1997.d01-25.x
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Culture in vitro of Rhododendron and Erysiphe sp. (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 44 (1995), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Successful dual cultures of a powdery mildew pathogen and Rhododendron ponticum plantlets were achieved using a tissue culture technique. Sporulation was observed within 1 week of inoculation. Comparative in vitro cultures were grown at 10, 15 and 20°C with host tissue transferred to fresh media at 2-monthly intervals. Viable conidia capable of infecting host tissue in vivo were obtained from cultures maintained at 10 and 15°C after culture for more than 6 months. Isolates from three sources (Auchincruive, Benmore and Perth) were introduced into culture. Cultures inoculated with Perth and Benmore isolates exhibited increased host growth at 10 and 15°C, respectively compared with control plantlets. These effects may result from pathogen-induced changes to host regulatory metabolism. The culture technique allows the long-term maintenance of pathogen cultures under sterile conditions without requiring subculture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1995.tb02787.x
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Effects of relative humidity, light intensity and photoperiod on the colony development of Erysiphe sp. on Rhododendron (2002)
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 51 (2002), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Growth and reproduction by powdery mildew pathogens is generally inhibited by decreasing relative humidity. With Erysiphe sp. on Rhododendron cv. Elizabeth, the initial stages of colony development were adversely affected by reducing the relative humidity from 100% to 70 and 85%. No significant effects on secondary or tertiary hyphal development were detected. Light intensity and photoperiod both had considerable effect on the induced resistance response of the host. Over the initial 5 days of colonization there were no significant differences between any of the treatments. After 13 days, however, expansion of fungal colonies at 180 photosynthetic active radiation (PAR) was limited solely to the area initially infested by primary hyphae. By comparison, in colonies grown at 80 PAR regardless of day length, secondary and tertiary hyphae had extended beyond the area first colonized. These effects resulted in differing morphologies, small colonies of densely packed hyphae formed at 180 PAR compared with open spreading colonies at 80 PAR.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.0032-0862.2001.x
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    Paper (German National Licenses)
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