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  • 1
    Electronic Resource
    Electronic Resource
    Strategies for virus resistance in plants
    Amsterdam : Elsevier
    Trends in Genetics 5 (1989), S. 56-60 
    Details
    ISSN: 0168-9525
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9525(89)90023-1
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  • 2
    Electronic Resource
    Electronic Resource
    Antisense nucleic acids and proteins: Fundamentals and applications - edited by Joseph N.M. Mol and Alexander R. van der Krol Marcel Dekker, 1991. 115.00 (viii + 231 pages) ISBN 0 8247 8516 9
    Amsterdam : Elsevier
    Trends in Genetics 9 (1993), S. 94-95 
    Details
    ISSN: 0168-9525
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9525(93)90232-7
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  • 3
    Electronic Resource
    Electronic Resource
    The sensitivity and specificity of a rapid nucleic acid hybridization method for the detection of potato virus X in crude sap samples (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 33 (1984), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A DNA copy (cDNA) of the common European strain (strain-group 3) potato virus X RNA (PVX3-RNA) was prepared by reverse transcription of PVX-RNA and amplified by cloning into E. coli plasmid pBR322. This DNA hybridizes with PVX-RNA and not at all to RNA of potato virus Y. A low amount of cross-hybridization was observed with strain-group 2 of PVX (PVX2). However, by hybridization of PVX2-RNA to restriction endonuclease fragments of PVX3-cDNA, it was demonstrated that cross-homology was not present in all regions of the PVX genome.The cloned PVX3-cDNA was hybridized to viral RNA in crude sap samples which had been spotted on to nitrocellulose membrane. The signal, which was assayed by autoradiography, allowed the detection of 50 pg of viral RNA. This value was not adversely affected by encapsidation of the RNA, nor by components of crude sap. The sap spot hybridization was as effective as enzyme-linked immunosorbent assay both in detection of small quantities of purified PVX3, and in screening of plants from a breeding programme for immunity to PVX3 infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1984.tb01331.x
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  • 4
    Electronic Resource
    Electronic Resource
    Sequence complexities of the poly(A)-containing mRNA in uninfected soybean root and the nodule tissue developed due to the infection by Rhizobium
    Amsterdam : Elsevier
    BBA Section Nucleic Acids And Protein Synthesis 563 (1979), S. 496-507 
    Details
    ISSN: 0005-2787
    Keywords: Leghemoglobin ; Nitrogen fixation ; Poly(A)-containing mRNA ; Rhizobium ; Root nodule symbiosis ; mRNA sequence
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2787(79)90068-6
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  • 5
    Electronic Resource
    Electronic Resource
    Novel strategies for engineering virus resistance in plants
    Amsterdam : Elsevier
    Current Opinion in Biotechnology 5 (1994), S. 117-124 
    Details
    ISSN: 0958-1669
    Keywords: BMV; brome mosaic virus ; CMV; sucumber mosaic virus ; CyRSV; cymbidium ringspot virus ; DI; defective-interfering ; PEBV; pea early browning virus ; PLRV; potato leaf roll virus ; PVX; potato virus X ; PVY; potato virus Y ; RdRp; RNA-dependent RNA polymerase ; TEV; tobacco etch virus ; TMV; tobacco mosaic virus ; TRV; tovacco rattle virus ; TYMV; turnip yellow mosaic virus ; ToSWV; tomato spotted wilt virus
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0958-1669(05)80023-0
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  • 6
    Electronic Resource
    Electronic Resource
    Novel strategies for engineering virus resistance in plants
    Amsterdam : Elsevier
    Current Opinion in Biotechnology 5 (1994), S. 117-124 
    Details
    ISSN: 0958-1669
    Keywords: BMV; brome mosaic virus ; CMV; sucumber mosaic virus ; CyRSV; cymbidium ringspot virus ; DI; defective-interfering ; PEBV; pea early browning virus ; PLRV; potato leaf roll virus ; PVX; potato virus X ; PVY; potato virus Y ; RdRp; RNA-dependent RNA polymerase ; TEV; tobacco etch virus ; TMV; tobacco mosaic virus ; TRV; tovacco rattle virus ; TYMV; turnip yellow mosaic virus ; ToSWV; tomato spotted wilt virus
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0958-1669(05)80023-0
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Expression of α-amylase and other gibberellin-regulated genes in aleurone tissue of developing wheat grains (1986)
    Springer
    Planta 169 (1986), S. 420-428 
    Details
    ISSN: 1432-2048
    Keywords: Aleurone ; α-Amylase ; Complementary DNA ; Gibberellin and gene expression ; Seed development ; Triticum (gibberellin)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Aleurone tissue from freshly harvested immature wheat grains (Triticum aestivum L. cv. Sappo) which is normally unresponsive to gibberellic acid can be made responsive by subjecting the tissue to a pre-incubation treatment in a simple buffered medium prior to the addition of the growth substance. The effectiveness of this treatment is dependent on grain age, with grains less than 15–20 days post anthesis failing to become converted to a responsive state whilst tissue from grains older than this become increasingly susceptible. Tissue from grains of a certain age (approx. 25–28 days post anthesis) produce small amounts of α-amylase following this treatment even in the absence of exogenously applied growth substance. Using different 32-labelled complementary-DNA probes for α-amylase in wheat it was demonstrated that the failure of freshly harvested tissue to produce α-amylase was correlated with the absence of the appropriate mRNA species. Inability to accumulate α-amylase mRNA in response to gibberellic acid was removed by the pre-iccubation treatment and also by enforced drying. The gibberellin-regulated expression of other unidentified genes also responds to pre-incubation or drying. Induction of gibberellin-responsiveness in immature aleurone cells did not extend to the secretion of acid phosphatase, protease and ribonuclease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392140
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  • 8
    Electronic Resource
    Electronic Resource
    Mapping of intra-locus duplications and introgressed DNA: aids to map-based cloning of genes from complex genomes illustrated by physical analysis of the Rx locus in tetraploid potato (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 679-689 
    Details
    ISSN: 1432-2242
    Keywords: Key words Map-based cloning ; BAC ; Rx ; PVX ; Potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We describe here novel approaches to high-resolution mapping of repeated and introgressed DNA which may prove generally useful in map-based cloning from complex genomes. These approaches were developed in order to clone the Rx locus in potato. First, we prepared a BAC library from a tetraploid plant carrying Rx in the duplex condition (Rx, Rx, rx, rx). BAC clones were then isolated with close markers on either side of Rx. However these clones did not extend across Rx: in the cloned DNA the closest markers to Rx were separated by single recombination events on either side of Rx. To bridge the gap we exploited the finding that the BAC clones on the right side of Rx contained resistance-gene homologues. Anticipating that there would be duplicated copies of these resistance gene homologues in the vicinity of Rx, we used low-stringency PCR conditions to identify additional markers. One of these markers was completely linked to Rx in our mapping population and was used to isolate a BAC (BAC77) that had not been previously identified by screening with Rx-flanking markers. Based on two criteria it was concluded that BAC77 spans Rx. There was a chromosomal recombination in one plant of our mapping population that separated the BAC77 right end from Rx. On the other side of Rx it was found that the BAC77 left end was outside the region of DNA carrying Rx that had been introgressed into potato cv Amaryl from a Solanum tuberosum ssp. andigena accession CPC1673.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051121
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  • 9
    Electronic Resource
    Electronic Resource
    High-resolution genetical and physical mapping of the Rx gene for extreme resistance to potato virus X in tetraploid potato (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 153-162 
    Details
    ISSN: 1432-2242
    Keywords: Key words PVX ; Resistance gene ; Mapping ; RFLP ; AFLP ; CAPS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The Rx locus in potato confers extreme resistance to PVX. In the F1 progeny of crosses between the PVX-susceptible cultivar Huinkel and the cultivar Cara (Rx genotype) there was a 1 : 1 segregation of PVX resistance, indicating that Rx in Cara is present in the simplex condition. Using potato and tomato RFLP markers, we mapped Rx in Cara to the distal end of chromosome XII at a different position to the previously mapped Rx1 locus. To generate a high-resolution linkage map in the vicinity of Rx a total 728 AFLP primer combinations were screened using DNA of bulked resistant and susceptible segregants. We also screened segregating populations for chromosomal recombination events linked to the Rx locus and identified 82 plants with recombination events close to Rx. Using these recombinant plants we have identified AFLPs that flank Rx and span an interval of 0.23 cM in a region of the genome where 1 cM corresponds to approximately 400 kb.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050543
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  • 10
    Electronic Resource
    Electronic Resource
    A saponin-detoxifying enzyme mediates suppression of plant defences (2002)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 418 (2002), S. 889-892 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Plant disease resistance can be conferred by constitutive features such as structural barriers or preformed antimicrobial secondary metabolites. Additional defence mechanisms are activated in response to pathogen attack and include localized cell death (the hypersensitive response). Pathogens ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature00950
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