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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Science Ltd
    Plant pathology 45 (1996), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A disease of pea caused by pea early-browning virus was first described in the Netherlands, and shortly afterwards was reported from England. The virus has since been reported from several other European countries, and also from North Africa. It can systemically infect crop legume species and the disease is locally important in pea in the Netherlands and England. The virus, a member of the Tobravirus genus, is transmitted through sandy or light-textured soils by species of trichodorid nematodes, and also through seed of pea and faba bean. Seed transmission is the most probable means of virus spread, especially over long distances, to previously uninfested soils. Aspects of the disease including symptoms, soil and seed transmission, detection, resistance and control, and characteristics of the pathogen, are reviewed.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 28 (1979), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In four out of five seasons in Wales, during 1970-76, the winter oat cvs Maris Osprey and Peniarth were very susceptible to oat mosaic virus, cv. Padarn was moderately susceptible and cvs Pendrwm and Century were resistant. However, plants of resistant cultivars were sometimes severely damaged and some symptomless plants contained virus particles. It is suggested that a minimum threshold of virus in the plant is necessary to induce symptoms and that this threshold is lower in susceptible than in resistant cultivars. Cvs Pendrwm and Century were also the most resistant to oat tubular virus. Sap from cv. Padarn induced most local lesions in the assay host Nicotiana clevelandii, indicating that oat tubular virus reached its highest concentration in this cultivar.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A DNA copy (cDNA) of the common European strain (strain-group 3) potato virus X RNA (PVX3-RNA) was prepared by reverse transcription of PVX-RNA and amplified by cloning into E. coli plasmid pBR322. This DNA hybridizes with PVX-RNA and not at all to RNA of potato virus Y. A low amount of cross-hybridization was observed with strain-group 2 of PVX (PVX2). However, by hybridization of PVX2-RNA to restriction endonuclease fragments of PVX3-cDNA, it was demonstrated that cross-homology was not present in all regions of the PVX genome.The cloned PVX3-cDNA was hybridized to viral RNA in crude sap samples which had been spotted on to nitrocellulose membrane. The signal, which was assayed by autoradiography, allowed the detection of 50 pg of viral RNA. This value was not adversely affected by encapsidation of the RNA, nor by components of crude sap. The sap spot hybridization was as effective as enzyme-linked immunosorbent assay both in detection of small quantities of purified PVX3, and in screening of plants from a breeding programme for immunity to PVX3 infection.
    Type of Medium: Electronic Resource
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