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  • 1
    ISSN: 1573-5168
    Keywords: fish ; Anguilla ; reproduction ; neuroendocrinology ; GnRH ; mGnRH ; cGnRH II ; steroid feedback
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Au moyen de dosages radioimmunologiques spécifiques de chacune des formes de GnRH présentes chez l'anguille européenne, Anguilla anguilla, (mGnRH et cGnRH II), nous avons comparé leur répartition dans l'hypophyse et les différentes parties du cerveau, chez l'anguille femelle argentée, ainsi que les modifications de leurs teneurs chez des femelles dont la maturation sexuelle est induite par un traitement à l'extrait hypophysaire de carpe. Chez les témoins, mGnRH est plus abondant que cGnRH II dans l'hypophyse, les lobes olfactifs et le téléncéphale, le di-et mésencéphale, alors que l'inverse est observé dans la partie postérieure du cerveau (mét- et mésencéphale). La maturation expérimentale des gonades entraine une augmentation significative des teneurs en mGnRH de l'hypophyse et des parties antérieures du cerveau; un tel effet positif n'est pas observé sur les faibles teneurs en cGnRH II qui, au contraire, sont diminuées. Ces données indiquent que le rétrocontrôle positif des stéroides gonadiques sur GnRH, que nous avions démontré précédemment, s'exercerait spécifiquement sur la forme mGnRH. Les différences dans la répartition et le contrôle de mGnRH et cGnRH II suggèrent que ces deux formes ont des rôles physiologiques différents chez l'anguille. L'élévation importante de mGnRH lors de la maturation sexuelle suggère l'implication principale de cette forme dans le contrôle neuroendocrine de la fonction de reproduction.
    Notes: Abstract Using specific radioimmunoassays for the two GnRH molecular forms present in the European eel, Anguilla anguilla, (mGnRH and cGnRH II), we compared their distributions in the pituitary and different parts of the brain of female silver eels, as well as the modifications of their levels in experimentally matured female eels (treated with carp pituitary extract). In control eels, mGnRH levels were higher than cGnRH II levels in the pituitary, olfactory lobes and telencephalon, di- and mesencephalon, while the opposite was found in the posterior part of the brain (met- and myelencephalon). Experimental sexual maturation of the gonads significantly increased mGnRH levels in the pituitary and anterior parts of the brain; such a positive effect was not observed on the low cGnRH II levels, which were, in contrast, reduced. These data indicate that the positive feedback of gonadal hormones on GnRH, that we previously demonstrated, would specifically affect the mGnRH form. The differential distribution and control of mGnRH and cGnRH II suggest that these two forms have different physiological roles in the eel. The large increase in mGnRH during sexual maturation suggests the prime implication of this form in the neuroendocrine control of reproduction.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5168
    Keywords: fish ; reproduction ; neuroendocrinology ; Anguilla ; sexual maturation ; feedback ; gonadoliberin ; gonadotropin ; castration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Treatment of sham-operated female silver eels with carp pituitary extract stimulated ovarian development and induced increases in pituitary gonadotropin (GTH) and gonadoliberin (GnRH) contents. Both effects of carp pituitary extract were abolished in ovariectomized eels, indicating the involvement of the gonads. Endogenous sexual steroids, the secretion of which was increased during sexual maturation, should be responsible for the stimulation of GTH and GnRH levels. Ovariectomy itself had no significant effect on pituitary GTH and GnRH contents, reflecting the fact that, at the silver stage, sexual steroid levels are too low to exert any significant effect on pituitary GTH and GnRH. The positive feedback control exerted by the gonads on GTH and GnRH levels during sexual maturation, in the eel as well as in some other teleosts, would produce an amplification of the pubertal stimulation of the hypothalamo-pituitary-gonadal axis.
    Type of Medium: Electronic Resource
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