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  • 1
    Electronic Resource
    Electronic Resource
    Molecular specification of cell lines in the embryo of Platynereis (Annelida) (1993)
    Springer
    Development genes and evolution 202 (1993), S. 260-269 
    Details
    ISSN: 1432-041X
    Keywords: Monoclonal antibodies ; Acetylcholinesterase ; Cell line ; Differentiation ; Spiralia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In this study we describe the site and moment of histospecific differentiation in developmental stages of the annelid Platynereis dumerilii by use of biochemical markers. The monoclonal antibody (mab) OI7 and uncloned hybridoma supernatants (pAb's) OI8, OI10, OI46 and OI69 recognize neural antigens that appear asynchronously during development. By an enzymatic test, acetylcholinesterase (AChE) was found specific for nervous tissue as well. The patterns of neural structures labelled by antibodies differ, however, from those revealed by AChE staining. Experimental inhibition of transcription (with actinomycin D) and of translation (using puromycin) demonstrate that the expression of histospecific neural markers depends on both zygotic transcription and subsequent translation. The mAb OI64 labels epidermal (and neural) gland cells. The antibody 4D9, raised against the engrailed protein of Drosophila, labels single rows of ciliated cells at the posterior border of segments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00363215
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  • 2
    Electronic Resource
    Electronic Resource
    Contrôle de la détermination et de la différenciation des caractères sexuels somatiques de Néréidiens (Annélides polychètes) (1974)
    Springer
    Development genes and evolution 175 (1974), S. 307-325 
    Details
    ISSN: 1432-041X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Les modalités de la détermination et de la différenciation du dimorphisme sexuel de Néréidiens (Annélides Polychètes) ont été recherchées chezNereis pelagica L. etPerinereis cultrifera G. par des expériences de décérébration, de castration (par irradiation X), de greffes et d'injection de produits génitaux. Les caractères sexuels somatiques des cirres parapodiaux (renflement et crénelures) se développent toujours conformément au sexe génétique quel que soit l'état génital de laNereis et la nature de l'intervention. La détermination des caractères sexuels somatiques des cirres parapodiaux apparaît donc comme étant très précoce (3 mois au moins avant la différenciation génitale) et stable. Par contre, les papilles pygidiales, caractère sexuel mâle dans les conditions normales, peuvent se différencier chez les très jeunes femelles dont l'épitoquie est déclenchée prématurément par décérébration et chez les femelles de tout âge dont les transformations hétéronéréidiennes sont réalisées en présence d'un milieu mâle (injection de produits génitaux, greffe sur un mâle). Les papilles pygidiales semblent donc déterminées précocement chez les deux sexes; leur différenciation est cependant inhibée chez les femelles au cours de la maturation génitale.
    Notes: Summary The modalities of determination and differentiation of sexual dimorphism of Nereids (Annelida Polychaeta) were investigated inNereis pelagica L. andPerinereis cultrifera G. with experiments involving decerebration, castration (by X irradiation), grafts or injection of genital products. The sexual somatic characters of the parapodial cirri (swelling and crenellations) always develop according to the genetic sex, whatever the genital state of the Nereis and the nature of the intervention. Therefore the determination of the sexual somatic characters of the parapodial cirri appear to be determined in a very precocious and stable way (at least 3 months before the genital differentiation). The pygidial papillae, a male sexual character in normal conditions, can be formed by very young females in which heteronereidation is realized prematurely by decerebration, and by females of any age in which heteronereid transformations are realized in a male environment (e.g. by injection of genital products, or grafting onto a male). Therefore the pygidial papillae may be determined precociously in either sex; however their differentiation is normally inhibited in females in the course of genital maturation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00574896
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  • 3
    Electronic Resource
    Electronic Resource
    Etude ultrastructurale des cirres parapodiaux de Nereidiens atoques (Annélides Polychètes) (1972)
    Springer
    Cell & tissue research 131 (1972), S. 309-327 
    Details
    ISSN: 1432-0878
    Keywords: Parapodial cirri ; Sensory cells ; Polychaete annelids ; Chemoreception
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les cirres parapodiaux deN. pelagica L.,P. cultrifera G. etPl. dumerilii Aud. et M. Edw. sont constitués par une expansion épidermique enveloppant une masse nerveuse comprenant un nerf axial, la névroglie et de nombreuses cellules nerveuses bipolaires. L'épiderme, caractérisé par la présence de formations de type myofilamentaire, est surmonté par une cuticule normale limitée extérieurement par une “membrane” riche en polysaccharides et dont les plis superficiels renferment un matériel tubulaire disposé en strates parallèles à la surface. Des cellules nerveuses bipolaires, dont l'aspect rappelle étroitement celui de cellules chimioréceptrices, relient le nerf à la surface du cirre. Elles sont soit groupées et portant chacune un seul cil, soit isolées et surmontée chacune de quatre cils; chaque cil est entouré par une couronne de microvillosités prolongeant la surface du bourgeon dendritique. Les cirres parapodiaux deNereis atoques se présentent done comme des chimiorécepteurs.
    Notes: Summary The parapodial cirri ofN. pelagica L.,P. cultrifera G. andPl. dumerilii Aud. and M. Edw. are formed by an epidermal expansion enveloping a nervous mass which includes an axial nerve, glia and many bipolar nerve cells. The epidermis, characterized by the presence of formations of myofilamentous type, is covered by a normal cuticle limited outside by a “membrane” rich in polysaccharides the superficial folds of which contain a tubular material disposed in layers parallel to the surface. The bipolar neurons, whose appearance closely resembles that of chemoreceptive cells, send a nerve to the surface of the cirrus. These cells are either grouped and each bearing a single cilium, or isolated and endowed with four cilia; each cilium is surrounded by a crown of microvilli which extend the surface of the dendritic hillock. Therefore, the parapodial cirri ofNereis appear to be chemoreceptors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00582854
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  • 4
    Electronic Resource
    Electronic Resource
    Multiplex RT-PCR method for the analysis of the expression of human sialyltransferases: application to breast cancer cells (1998)
    Springer
    Glycoconjugate journal 15 (1998), S. 19-27 
    Details
    ISSN: 1573-4986
    Keywords: sialyltransferases ; breast cancer cells ; multiplex RT-PCR ; glycosyltransferases ; bp, base pair ; GAPDH, glyceraldehyde-3-phosphate dehydrogenase ; kb, kilobase ; PNA, Peanut (Arachis hypogaea) agglutinin ; RT-PCR, reverse transcription-polymerase chain reaction ; TBE, Tris base 0.13 M, boric acid 45 mM ; Na2EDTA 2.55 mM, pH 8.8 buffer ; Tm, melting temperature ; the nomenclature of sialyltransferases is based on that of Tsuji et al. [36]: ST3Gal I: CMP-NeuAc, Gal beta 1-3GalNAc alpha 2,3-sialyltransferase, EC 2.4.99.4 ; ST3Gal III: CMP-NeuAc, Gal beta 1-3/4GlcNAc alpha 2,3-sialyltransferase, EC 2.4.99.6 ; ST3Gal IV: CMP-NeuAc, Gal beta 1-4GlcNAc or Gal beta 1-3GalNAc alpha 2,3-sialyltransferase, EC 2.4.99. ; ST6Gal I: CMP-NeuAc, Gal beta 1-4GlcNAc alpha 2,6-sialyltransferase, EC 2.4.99.1.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract In many cases of human cancer, the appearance of hypersialylated glycan structures is related to a precise stage of the disease; this may depend on altered regulation of one or more sialyltransferases genes. Since several distinct sialyltransferase enzymes arising from different unique genes transfer sialic acid residues in the same linkage onto the same acceptor, it is impossible to precisely determine which enzyme is involved in the observed phenotype based on enzymatic assays. We have developed a very sensitive and highly reproducible multiplex reverse transcriptase-polymerase chain reaction technique in order to monitor the expression of four human sialyltransferases genes ST6Gal I, ST3Gal I, ST3Gal III and ST3Gal IV in small cell samples. Multiplex PCR amplification using specific primers for each sialyltransferase and detection of amplification products by polyacrylamide gel electrophoresis is a method that is fast and easy to handle and has proven to be useful for establishing sialyltransferase patterns of expression in breast immortalized cell line HBL100 as well as in breast cancer cell lines MCF-7/6, MCF-7/AZ and MDA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006983214918
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