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  • 1
    Electronic Resource
    Electronic Resource
    Transformation of Mycobacterium aurum and Mycobacterium smegmatis with the broad host-range Gram-negative cosmid vector pJRD215 (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 5 (1991), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The transformation of Mycobacterium aurum and Mycobacterium smegmatis with the Gram-negative RSF1010-derived cosmid pJRD215 is described. The plasmid is stably maintained in both species and the antibiotic resistance determinants for kanamycin and streptomycin are expressed. Southern blot analysis shows that rearrangements take place both in M. aurum and in M. smegmatis. The use of pJRD215 in mycobacterial cloning systems is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1991.tb00803.x
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  • 2
    Electronic Resource
    Electronic Resource
    An in vivo analysis of the energetics of aldose oxidation by Acinetobacter calcoaceticus (1987)
    Springer
    Applied microbiology and biotechnology 26 (1987), S. 560-567 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A continuous culture study was made of the energetics of oxidation of various aldose sugars by Acinetobacter calcoaceticus LMD 79.41. The consumption of aldoses during carbon- and energy-limited growth of the organism on mixtures of acetate and an aldose was independent of the pH of the culture. Acid production, however, was strongly dependent on this parameter. It is shown that aldose consumption without concurrent acid production is due to formation of the corresponding lactone, the hydrolysis of which is pH-dependent. The cell yield of A. calcoaceticus on mixtures of acetate and glucose or xylose was much higher than during growth on acetate alone. This increase in cell yield was, however, dependent on the pH of the culture. Only at pH values which permitted a high rate of lactone hydrolysis an enhancement of the cell yield was observed. These results suggest that lactone hydrolysis has an important bearing on the efficiency of periplasmic oxidation of aldoses in bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00253033
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  • 3
    Electronic Resource
    Electronic Resource
    Synthesis of optically pure 1,2-epoxypropane by microbial asymmetric reduction of chloroacetone (1992)
    Springer
    Applied microbiology and biotechnology 38 (1992), S. 297-300 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A number of bacteria and yeast was screened for asymmetric reduction of prochiral chloroacetone into chiral 1-chloro-2-propanol, which is chemically convertible into chiral 1,2-epoxypropane. In this way Rhodotorula glutinis produced optically pure S-1,2-epoxypropane with 98% enantiomeric excess and in a relatively high final concentration. The enzyme that catalysed the asymmetric reduction was an NAD(P)H-dependent alcohol dehydrogenase. Reduction of racemic 3-chloro-2-butanone resulted in mixtures of cis and trans-2,3-epoxybutane, indicating that no enantioselective reduction of this haloketone occurred.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00170075
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  • 4
    Electronic Resource
    Electronic Resource
    Effect of various co-substrates on 1,2-epoxypropane formation from propene by ethene-utilizing Mycobacteria (1987)
    Springer
    Applied microbiology and biotechnology 26 (1987), S. 434-438 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Ethanol, ethylacetate and ethene were tested as no-substrates to improve prolonged 1,2-epoxypropane formation from propene by immobilized cells of three strains (Eu1, 2W, E3) of ethene-utilizing Mycobacteria. Cells grown either on ethene or on both ethene and ethanol were immobilised on lava and the effect of co-substrates on 1,2-epoxypropane formation from propene was recorded in a gas/solid bioreactor. The rate of 1,2-epoxypropane formation by immobilized cells of strains Eu1 and 2W was significantly enhanced when ethanol or ethylacetate were supplied simultaneously with propene, while biocatalysis during a prolonged period of time was achieved in the presence of ethylacetate. Co-substrates had no beneficial effect on 1,2-epoxypropane formation by strain E3. It was possible to increase the 1,2-epoxypropane formation rate by immobilized cells over a period of three weeks of operation by supplying propene and ethene alternately.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00253527
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation and description of a non-motile, fusiform, stalked bacterium, a representatieve of a new genus (1970)
    Springer
    Antonie van Leeuwenhoek 36 (1970), S. 397-407 
    Details
    ISSN: 1572-9699
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A single strain representing the fusiform group of caulobacters first described by Henrici and Johnson has been isolated from a freshwater pond. Like the genusCaulobacter this is a chemo-organotrophic bacterium that has one polar prostheca, a stalk in the sense that its apical holdfast permits the cell to attach to solid substrates. Fine structure studies reveal, however, that the prostheca of this organism contains typical cellular constituents, not the membranous material found in the stalks ofCaulobacter andAsticcacaulis. The organism also differs from the other caulobacters in having no motile stage and no dimorphic life cycle (both daughter cells are stalked at the time of division). Because only one strain has been isolated no nomenclatural proposals are made, but sufficient evidence is presented to indicate that this is a representative of a new genus of the Schizomycetes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02069040
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  • 6
    Electronic Resource
    Electronic Resource
    Inactivation of alkene oxidation by epoxides in alkene-and alkane-grown bacteria (1985)
    Springer
    Applied microbiology and biotechnology 22 (1985), S. 428-433 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The oxidation of propene by resting-cells of ethene-grown Mycobacterium E3 was inactivated by 1,2-epoxypropane. Inactivation increased with increasing epoxide concentrations with 50% inactivation at approximately 30 mM epoxide. Other lower epoxides as epoxyethane and 1,2-epoxybutane also inactivated oxidation of propene as well as of other alkenes. Propene oxidation by resting-cells of ethane-grown Mycobacterium E20 and resting-cells of methane-grown Methylosinus trichosporium OB3b was inactivated for 50% at much lower 1,2-epoxypropane concentrations of approximately 1 and 3 mM respectively. It was demonstrated that in vivo the predominant effect of 1,2-epoxypropane was on the epoxidizing enzyme, i.e. alkene mono-oxygenase (strain E3), alkane mono-oxygenase (strain E20) and methane mono-oxygenase (methylotroph) and that the effect of the epoxide on the alkene mono-oxygenase was irreversible.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252785
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  • 7
    Electronic Resource
    Electronic Resource
    Bioformation of 4-hydroxybenzoate from 4-chlorobenzoate by Alcaligenes denitrificans NTB-1 (1986)
    Springer
    Applied microbiology and biotechnology 25 (1986), S. 289-294 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Strain NTB-1, identified as a Alcaligenes denitrificans sp., was isolated from a mixture of soil and sewage samples using 4-chlorobenzoate as sole carbon and energy source. Simultaneous adaptation experiments and enzyme studies revealed that 4-chlorobenzoate was converted to 4-hydroxybenzoate which was further oxidized yielding 3,4-dihydroxybenzoate. Bioformation of 4-hydroxybenzoate from 4-chlorobenzoate when 4-chlorobenzoate-grown cells were incubated with 4-chlorobenzoate under conditions of low and controlled oxygen concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00253665
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  • 8
    Electronic Resource
    Electronic Resource
    Characterization of a Mycobacterium sp. and a Xanthobacter sp. for the removal of vinyl chloride and 1,2-dichloroethane from waste gases (1992)
    Springer
    Applied microbiology and biotechnology 37 (1992), S. 796-801 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Mycobacterium aurum L1 and Xanthobacter autotrophicus GJ10 were characterized with respect to their potential use in the biological treatment of waste gases containing vinyl chloride (VC) and 1,2-dichloroethane (DE). VC at a concentration of 125 g m −3 in the gas phase was not toxic but DE at 22 g m −3 already resulted in a decreased growth rate. Kinetic properties of washed cells were determined and chemostat cultures were run to optimize the medium for VC removal. Using a mixed culture of the two strains, simultaneous removal and mineralization of VC and DE was demonstrated. The affinity constants of growing cells found for the two substrates are, however, significantly higher than the maximal allowable concentrations of VC and DE in waste gases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00174848
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  • 9
    Electronic Resource
    Electronic Resource
    Dithiol- and NAD-dependent degradation of epoxyalkanes by Xanthobacter Py2 (1995)
    Springer
    Applied microbiology and biotechnology 42 (1995), S. 775-781 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A broad range of epoxyalkanes was converted into the corresponding ketones by cell extracts of Xanthobacter Py2. Both 1,2- and 2,3-epoxyalkanes were degraded and in addition, the degradation of 2,3-epoxyalkanes in all cases was highly enantioselective. Conversion of a deuterium-labelled substrate indicated that the ketone product was probably formed indirectly via an hydroxy intermediate. Degradation of epoxyalkanes by Xanthobacter Py2 was dependent on both NAD and another, not yet identified, cofactor that was present in the low-molecular-mass fraction (LMF) of propene-grown cells. It is proposed that the LMF was involved in a reductive reaction step since it could be replaced by dithiothreitol (DTT) and various other dithiol compounds. Epoxyalkane-degrading activity was inhibited by the sulphhydryl blocking reagent N-ethylmaleimide (NEM). Inhibition by NEM and stimulation by LMF, DTT and other dithiols was effective only in the simultaneous presence of NAD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00171961
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  • 10
    Electronic Resource
    Electronic Resource
    The involvement of an enantioselective transaminase in the metabolism of D-3- and D-4-hydroxyphenylglycine inPseudomonas putida LW-4 (1988)
    Springer
    Applied microbiology and biotechnology 29 (1988), S. 224-230 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Pseudomonas putida LW-4, isolated on D-phenylglycine as sole carbon and energy source, was also able to grow on D-3-and D-4-hydroxyphenylglycine. Both D-3-and D-4-hydroxyphenylglycine were initially converted to the corresponding hydroxyphenylglyoxylates by means of an enantioselective transaminase. Subsequently, the hydroxyphenylglyoxylates were decarboxylated and then oxidized to 3-and 4-hydroxybenzoate, respectively. These latter compounds in turn were oxidized by NADPH-dependent hydroxylases to protocatechuate, which was further oxidized via an intradiol cleavage. Preliminary experiments with cell extracts in which the 4-hydroxyphenylglyoxylate decarboxylase was partially removed by an ammonium sulfate fractionation showed that D-4-hydroxyphenylglyoxycine could be formed from 4-hydroxyphenylglyoxylate by the enantioselective transaminase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01982906
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