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  • 1
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Plant Physiology and Plant Molecular Biology 44 (1993), S. 435-456 
    ISSN: 1040-2519
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 101 (2000), S. 165-172 
    ISSN: 1432-2242
    Keywords: Key words Medicago sativa ; Autotetraploid linkage map ; Microsatellites SSR ; Inbreeding depression ; Single dose allele (SDA) analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Cultivated alfalfa (Medicago sativa) is an autotetraploid. However, all three existing alfalfa genetic maps resulted from crosses of diploid alfalfa. The current study was undertaken to evaluate the use of Simple Sequence Repeat (SSR) DNA markers for mapping in diploid and tetraploid alfalfa. Ten SSR markers were incorporated into an existing F2 diploid alfalfa RFLP map and also mapped in an F2 tetraploid population. The tetraploid population had two to four alleles in each of the loci examined. The segregation of these alleles in the tetraploid mapping population generally was clear and easy to interpret. Because of the complexity of tetrasomic linkage analysis and a lack of computer software to accommodate it, linkage relationships at the tetraploid level were determined using a single-dose allele (SDA) analysis, where the presence or absence of each allele was scored independently of the other alleles at the same locus. The SDA diploid map was also constructed to compare mapping using SDA to the standard co-dominant method. Linkage groups were generally conserved among the tetraploid and the two diploid linkage maps, except for segments where severe segregation distortion was present. Segregation distortion, which was present in both tetraploid and diploid populations, probably resulted from inbreeding depression. The ease of analysis together with the abundance of SSR loci in the alfalfa genome indicated that SSR markers should be a useful tool for mapping tetraploid alfalfa.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-1561
    Keywords: Neotyphodium ; Acremonium ; ergot alkaloids ; diallel cross ; heritability ; fescue toxicosis ; endophyte ; tall fescue ; mutualism ; symbiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Ergot alkaloids in endophyte-infected (E+) tall fescue (Festuca arundinacea) are responsible for livestock toxicosis. The host plant is capable of modifying the extent to which endophyte produces ergot alkaloids, possibly via endophyte nutrition by the host. Endophytes vary in alkaloid production. Maintaining the E+ tall fescue is essential for plant survival. Therefore, crop scientists are interested in reducing alkaloid concentration of E+ tall fescue. The objectives of this study were to determine maternal and paternal effects as an estimate of plant and endophyte genotype interactions for ergot alkaloid phenotype and the heritability of the ergot alkaloid trait in divergently selected E+ tall fescue populations. Diallel crosses were conducted among four tall fescue genotypes that varied in alkaloid concentration. Five of six crosses had progeny means less than the parental means when genotypes with lowest alkaloid were the female parents. Four of six crosses had progeny means similar to the parental means of crosses when high alkaloid genotypes were the female parents. Large ranges in progeny suggest an interaction between endophyte and plant affects alkaloid phenotype. In a second experiment, a tall fescue population was screened for alkaloid concentration. Low- and high-alkaloid plants were selected and poly-crossed within each class. Mean alkaloid concentrations of the high- and low-alkaloid progeny were higher or lower than the parental population. The trait was highly heritable and alkaloid concentration was reduced by 86% in the low-alkaloid population after two generations of selection.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 83 (1991), S. 89-96 
    ISSN: 1432-2242
    Keywords: RFLP ; Alfalfa ; Genetic diversity ; Phylogenetic tree ; Gene duplication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Alfalfa (Medicago sativa L.) is a major forage crop throughout the world. Although alfalfa has many desirable traits, continued breeding is required to incorporate pest resistances and other traits. We conducted this study to determine the amount of restriction fragment length polymorphism (RFLP) variability present within and between diploid and tetraploid alfalfa populations, and whether or not this variability is sufficient for construction of an RFLP map. Diploid plants from M. sativa ssp. falcata, ssp. coerulea, and ssp. sativa and tetraploid spp. sativa cultivars ‘Apollo,’ ‘Florida 77,’ and ‘Spredor 2’ were included. A total of 19 cDNA clones was probed onto genomic Southern blots containing DNA digested by EcoRI, HindIII, or BamHI. Phylogenetic trees were produced, based on parsimony analysis of shared restriction fragments. Evidence for extensive gene duplication was found; most probes detected complex patterns of restriction fragments. Large amounts of variation are present within all diploid subspecies. M. sativa ssp. falcata plants formed clusters distinct from ssp. sativa or ssp. coerulea plants, which were not distinctly clustered. Some M. sativa ssp. falcata plants were more similar to the other groups than to other plants within ssp. falcata. Variation among tetraploid cultivars showed that Florida 77 and Apollo had more similarities than either showed with Spredor 2. All three cultivars showed large within-population variation, with Apollo being the most diverse and Spredor 2 the least. Based on these results, development of an RFLP map at the diploid level appears possible. Also, differentiation of cultivars, particularly ones of divergent origin, seems possible based on RFLP patterns.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 116 (1989), S. 283-285 
    ISSN: 1573-5036
    Keywords: acetylene reduction ; acid-tolerant germplasm ; Al ; Al toxicity ; lucerne ; Medicago sativa ; N2-fixation ; nodulation ; top dry weight
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Alfalfa (Medicago sativa L.) growth and nodulation in acid soil is reduced because the plant and its bacterial symbiontRhizobium meliloti cannot tolerate acid, aluminum-rich soil. A study was conducted to determine if a relatively acid-tolerant alfalfa germplasm combined with a relatively acid-tolerantR. meliloti strain could overcome these limitations. In a light room study, an acid-tolerant alfalfa germplasm inoculated with a more acid-tolerantR. meliloti strain produced greater top growth, nodule number and weight, and acetylene reduction values in an unlimed soil (pH 4.6) than the same germplasm inoculated with a relatively acid-sensitiveR. meliloti strain or an acid-sensitive germplasm inoculated with either a relatively acid-tolerant or acid-sensitiveR. meliloti strain.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 52 (1979), S. 585-590 
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Azospirillum brasilense ; Guineagrass ; Plant dry weight ; Plant nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects on plant dry weight and acetylene reduction activity after applyingAzospirillum brasilense (strain 13t) to guineagrass,Panicum maximum Jacq., grown in clay pots under greenhouse conditions, are reported and discussed.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 60 (1981), S. 205-211 
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Acid soil ; K application ; Liming ; N2-fixation ; Nodulation ; P application ; Rhizobium meliloti ; Root dry weight ; Shoot dry weight
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Alfalfa plants,Medicago sativa L., were selected from the Florida 66 cultivar for vigor in an acid (pH 4.4, Al≥.4 meq/100 g) and a limed, fertilized (pH 6.5, Al=0 meq/100 g, P and K added) Cecil topsoil. The selected plants were intermated by selection condition to achieve two germplasms, acid selected (A-1) and limed, fertile selected (L-1). ARhizobium meliloti strain (79-4s) was isolated from a high acetylene reducing nodule from a plant in a similar acid soil. The germplasms and the Rhizobium strain were then tested in greenhouse pots for agronomic performance under a variety of soil pH and fertility conditions. The 79-4s inoculum, as well as commercially prepared inoculum, gave better plant yield and acetylene reduction (N2-fixation) at all harvests when compared to a sterile peat control, but the commercial inoculum was the best inoculum treatment. The A-1 germplasm produced higher shoot dry weight at the final harvest than did the L-1 germplasm at all soil pH’s when P and K were applied at the highest rates. The A-1 germplasm also had better root weight (mainly fibrous roots) and acetylene reduction in these soil conditions. The two germplasms appear to be genetically distinct and respond differently depending on soil pH and fertility conditions.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 65 (1982), S. 27-33 
    ISSN: 1573-5036
    Keywords: Acid soil ; Acid tolerant germplasm ; Liming ; Nodulation ; P application ; Root dry weight ; Top dry weight
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Seedlings of a broad based population of alfalfa, (Medicago sativa L.), were visually selected for 2 generations (cycles) in an acid Cecil soil (pH=4.8). Simultaneously, 2 generations of selections were made in a limed Cecil soil (pH=6.2) amended with phosphorus. When tested in acid soil (pH=4.8) with added P, the cycle 2 acid selections yielded significantly more top yield than either the limed selections or the original seed. When grown in the limed soil (pH=6.2) without added P, the acid selections yielded significantly less. There were no significant differences among selections in other soil conditions (pH=4.8, no P added and pH=6.2, P added). Root weight and length and nodule fresh weight correlated well with top yield in all treatments. The yield of the 10 parent varieties and polycrossed seed of their cycle 1 acid and cycle 1 limed selections were compared under two soil conditions (acid and limed). The results of this test indicate that some varieties were more responsive to acid soil selection than other. Overall, none of the 10 varieties showed any decreases in total top yield due to one cycle of selection in either the acid or limed soil conditions.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 74 (1983), S. 431-436 
    ISSN: 1573-5036
    Keywords: Al ; Liming ; Lucerne ; Mn ; N content ; P application ; Selection ; Top-growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Previous selection of alfalfa for productivity in acid soil resulted in germplasms with more acid soil tolerance, but which required high P levels to be responsive. In this investigation, the field performance of alfalfa germplasms (selected in either acid or limed soil conditions) was evaluated over three years at varying soil pH and P levels. Advancement made by selection in high and low yielding soil environments was also determined. Increasing soil pH from 4.8 to 7.2 increased alfalfa dry matter yield as did increasing P application from 80 to 200 kg P ha−1. At the acid end, levels of Al and Mn were not found to be toxic, but N content of tissue increased with increasing soil pH indicating better N2-fixation due probably to improved Mo availability. In one of the three years, the acid selections yielded significantly higher than the base population when data were pooled for all soil conditions, but there was no advantage in low pH soil. Acid selections were also more responsive than limed selections to P application over all pH levels. Acid soil selection appeared advantageous in increasing the productivity of alfalfa for use in high yielding environments.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 65 (1982), S. 187-192 
    ISSN: 1573-5036
    Keywords: Al toxicity ; Gypsum ; Liming ; Lucerne ; Top growth dry weight ; Water extraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Rooting into acid subsoils would be a desirable trait for alfalfa which should result in better water extraction and yield. In this study, the rooting depth into acid subsoils and top yield of alfalfa plants selected for acid tolerance were investigated in a repacked profile with a limed, fertile topsoil, but unamended, acid subsoil. The effects of subsoil modification by CaCO3 and CaSO4·2H2O addition on alfalfa rooting, top growth, and water extraction were also studied. Plants from acid selections rooted deeper into acid subsoil when compared to control plants (selected under limed conditions). However, the reverse response was found in the CaSO4·2H2O treated subsoil. There were no differences among selections for total top yield for any subsoil treatment. Water extraction from the lower subsoil and top growth yield (data pooled by selections) were improved mainly in the subsoil treatment containing the highest addition of CaCO3.
    Type of Medium: Electronic Resource
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