ISSN:
0021-9541
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
Some properties of the enzyme phosphoglucose isomerase (PGI) from the foetal lung fibroblast strain MRC-5 have been investigated throughout the in vitro lifespan of this cell strain.No significant age-related alterations in specific activity or thermostability of PGI could be detected. Titration of enzymatic activity with antibody directed against purified PGI showed no detectable differences in PGI from extracts of early passage cells compared with enzyme from senescent cells.The effect of p-fluorophenylalanine incorporation on PGI was examined in early passage fibroblasts. Thermostability studies showed increased heat lability of PGI from analogue treated cells when compared with enzyme from control cells at the same passage. However, no inactive PGI protein could be detected by antiserum titration in extracts from analogue-treated cells.The results indicate that no significant amount of altered or inactive PGI is produced in ageing fibroblasts.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcp.1040940114
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