ISSN:
1432-1203
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary The polymorphic locus D19S11 consists of four closely linked RFLPs: α, β, δ and γ on chromosome 19p13.2→19cen, revealed by subclones p13-1-82 and p13-2-21 from cosmid 1–13. Here, we report that p13-1-25, an additional subclone of c1-13, reveals three insertion/deletion RFLPs, α, ε, and ϕ, at the D19S11 locus. In situ hybridization of p13-1-25 to metaphase chromosomes from a carrier of a 19/X translocation with a breakpoint near the centromere confirms localization of D19S11 to 19p. Studies with hydatidiform moles have generated assignments of specific restriction fragments to these three loci, and genotypic studies in three-generation families have indicated that they are closely linked. Loci α (also detected by p13-1-82) and ϕ each have but two common alleles, whereas ε has at least 33 alleles, including a null allele. Fifty unrelated individuals tested displayed unique fragment patterns on Taq I blots probed with p13-1-25. Applications of this probe include monitoring loss of chromosome 19 during tumorigenesis, monitoring engraftment of donor bone marrow after transplantation, testing for paternity, and mapping disease genes on chromosome 19.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00283056
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