ISSN:
1365-3083
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
In this study the idiotype-positive B cells of one patient with smouldering multiple mycloma (IgGx) and of one patient with multiple myeloma (IgGλ) were analysed phenotypically and functionally. As regards the expression of B cell-associated differentiation antigen and size distribution, the idiotype-positive B ells resembled normal IgG-bearing blood B Cells. In functional studies the lymphocytes were cultured in vitro with Staphylococcus aureus. pokeweed mitogen, T-cell factors, or combinations of those. After culture, proliferation and differentiation of the idiotype-positive B cells were measured by autoradiography, an idiotype-specific ELISA, and B spot ELISA. The results show that idiotype-positive B Cell of both patients are able to proliferate after simulation in vitro. In contrast to their normal counterparts, however, almost no increase in the amount of secreted idiotypic IgG could be induced. This suggests that the idiotype-positive blood B cells have lost some of their ability to respond to exogenous stimuli.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-3083.1988.tb01513.x
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