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  • 1
    ISSN: 1432-0584
    Keywords: Thalassaemia ; Scanning electron microscopy ; Transmission electron microscopy ; Freeze-etching electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The reported scanning (SEM), transmission (TS), and freeze-etching (FE) electron microscopic studies have agreed in confirming that in thalassaemic erythrocytes, previously incubated with brilliant cresyl blue (BCB), the unpaired alpha chains precipitate in the central portions of the cell whereas excess beta chains locate in the submembranous regions. This is due to the fact that beta chains, possessing two thiols instead of only one (as in alpha chains), are more liable to bind to similar groups contained in the inner red cell leaflet. Less soluble alpha chains tend to form inter-chain bridges and thus precipitate centrally. SEM observations have given evidence that on the surface of the affected red cells denaturated alpha chains give rise to large and shallow invaginations whereas denatured beta chains lead to a diffuse wrinkled appearance. The causes of the different SEM aspects have been suggested.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of thermal analysis and calorimetry 38 (1992), S. 505-514 
    ISSN: 1572-8943
    Keywords: ageing ; differential scanning calorimetry ; dynamic thermogravimetry ; electron microscopy ; tendon collagen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Mittels DSC, TG und Elektronenmikroskop wurden unverkalkte Beugesehnenregionen von Truthahnbeinen in Abhängigkeit des Alters untersucht. Rehydratierte Proben zeigen mit zunehmendem Alter ein Ansteigen der thermischen Stabilität. Der Wert von δHD sinkt von 11 cal/g in den ersten Lebenswochen auf 7 cal/g nach der 11.Woche. Nach etwa 11 Wochen geht der Durchmesser der Kollagenfibrillen vom einer eingipfeligen in eine multigipfelige Verteilung über. Weder die DSC-, noch die TG-DTG-Kurven von getrockneten Proben zeigen irgendwelche nennenswerten Unterschiede in Abhängigkeit vom Alter. Die änderungen des thermischen Verhaltens rehydratierter Proben und der Verteilung des Fibrillendurchmessers können den änderungen der Bindung des Wassers in Abhängigkeit vom Alter zugeschrieben werden.
    Notes: Abstract A differential scanning calorimetric, thermogravimetric and electron microscopic investigation has been carried out on the uncalcified areas of turkey leg flexor tendon as a function of age. Rehydrated samples exhibit an increase of thermal stability with age. The δHD values drop from about 11 cal·g−1 in the first weeks of life down to 7 cal·g−1 after the 11th week. At about 11 weeks, the collagen fibril diameter distribution passes from unimodal to multimodal. The DSC curves as well as the TG-DTG curves recorded from dried samples do not show any appreciable difference with ageing. The variations in thermal behaviour of rehydrated samples and fibril diameter distribution could be related to modifications in water binding with ageing.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0878
    Keywords: Collagen microfibrillar arrangement ; Freeze-fracture ; Tail tendon ; X-ray diffraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The microfibrillar arrangement in collagen fibrils of tendons in the tail of the rat was examined by electron microscopy and X-ray diffraction. Fresh and air-dried collagen fibers were examined in unstretched and stretched conditions. The results demonstrate that the microfibrils have a course parallel to the longitudinal axis of the collagen fibrils. The influence of stretching and hydration of the samples on the orientation of fibrils and microfibrils is also assessed.
    Type of Medium: Electronic Resource
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