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  • 1
    Electronic Resource
    Electronic Resource
    Effects of growth temperature on maximal specific growth rate, yield, maintenance, and death rate in glucose-limited continuous culture of the thermophilic Bacillus caldotenax (1980)
    Springer
    Applied microbiology and biotechnology 10 (1980), S. 303-315 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The influence of temperature on the growth of the theromophilic Bacillus caldotenax was investigated using chemostat techniques and a chemically defined minimal medium. All determined growth constants, that is maximal specific growth rate, yield and maintenance, were temperature dependent. It was striking that the very large maintenance requirement was about 10 times higher than for mesophilic cells under equivalent conditions. A death rate, which was very substantial at optimal and supraoptimal growth temperatures, was estimated by comparing the maintenance for substrate and oxygen. There was no indication for a thermoadaptation as postulated by Haberstich and Zuber (1974).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00498727
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The growth behaviour of Thermus aquaticus in continuous cultivation (1982)
    Springer
    Applied microbiology and biotechnology 15 (1982), S. 69-74 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The growth of the yellow pigmented non-sporulating caldoactive bacterium Thermus aquaticus was investigated in different culture vessels and using differnt culture techniques. Each combination of these two variables led to very specific characteristic behaviour of the culture. A synthetic medium for a white cell type of T. aquaticus was optimized by means of pulse and medium-shift techniques. The main kinetic parameters of the organism have been determined to be μ=1.62h−1 and Y (glucose)=0.4 g g−1 at T=68 °C and pH=7.3. In complex medium only a mixed population of white and yellow cells could be cultivated. The cell yield was shown to be very low (Y=0.02 g g−1) due to incomplete substrate utilisation, but a very high maximal specific growth rate was determined (μ max=3.5h−1) at 75 °C and pH=7.3. The maintenance coefficient for oxygen uptake was approximately Mo=16 mMol g−1 h−1. A discussion of the problems arising in the cultivation of thermophilic microorganisms with respect to their physiology and stability is given.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00499509
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  • 3
    Electronic Resource
    Electronic Resource
    Growth kinetics of Thermus thermophilus (1982)
    Springer
    Applied microbiology and biotechnology 15 (1982), S. 75-82 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The nonsporulating extreme thermophile Thermus thermophilus was grown in continuous culture at dilution rates up to 2.65 h−1 at 75°C and pH 6.9 on complex medium. Concomitantly very low yield (Y=0.12 g cell dry weight g−1 utilized organic carbon) and incomplete substrate utilization (always less than 45%) were found. In batch cultures T. thermophilus could be grown with μ max =h−1, in shake flasks only with μ max =h−1 with the same low yield and incomplete substrate utilization. Stable steady states at 84δC and 45°C were realized at a dilution rate of 0.3 h−1 whereas at 86°C and 40°C no growth could be detected. Artefacts arising from wall growth (in bioreactors) or improper materials must be ruled out. Inhibition of growth by organic substrates was demonstrated at low concentrations: a decrease in the yield obtained was found when more than 0.7 gl−1 of meat extract were supplied in the medium. The maintenance requirement for oxygen is potentially very high and was determined to be 10 to 15 mmol g−1 h−1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00499510
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  • 4
    Electronic Resource
    Electronic Resource
    Population distribution of aerobic extremely thermophilic microorganisms in an icelandic natural hot spring (1982)
    Springer
    Applied microbiology and biotechnology 16 (1982), S. 151-156 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The aerobic microbial population of natural hot spring water was investigated by means of taxonomic analyses of 33 characteristic properties. Twenty-eight strains isolated in Iceland were compared with 3 Thermus and 4 Bacillus reference strains. The results showed a high variability in the characteristics of bacteria isolated from nearby sampling areas. No typical sampling-area-specific or temperature-specific populations were found. These findings suggested high diversity of thermophiles due to environmental factors. In this report adaptation mechanisms are postulated and discussed in relation to the growth behaviour of Thermus strains in the natural environment and in continuous cultivation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00500745
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  • 5
    Electronic Resource
    Electronic Resource
    Equipment and growth inhibition of thermophilic bacteria (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 24 (1982), S. 2597-2599 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260241123
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    Paper (German National Licenses)
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