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  • 1
    Electronic Resource
    Electronic Resource
    Electroporation-mediated DNA delivery to seedling tissues ofPhaseolus vulgaris L. (common bean) (1995)
    Springer
    Plant cell reports 15 (1995), S. 119-124 
    Details
    ISSN: 1432-203X
    Keywords: direct gene transfer ; Fabaceae ; grain legumes ; intact tissue ; transient gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary DNA was delivered to intact embryonic axes of the legumePhaseolus vulgaris L. through electroporation. Expression of the ß-glucuronidase reporter gene was observed in hypocotyl and epicotyl tissue in a spot-like manner. Transgene expression was high when a single pulse of 260 ms at a field strength of 225 V·cm−1 was applied but could be achieved within a wide range of electrical conditions. Linearization of plasmid DNA greatly enhanced transient expression levels. The procedure was successful for embryonic axes of all testedP. vulgaris cultivars, for similar explants of several large-seeded leguminous species, as well as for some other tissues ofP. vulgaris.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01690267
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Shoot regeneration in long-term callus cultures derived from mature flowering plants of Cyclamen persicum Mill. (1996)
    Springer
    Plant cell reports 15 (1996), S. 545-548 
    Details
    ISSN: 1432-203X
    Keywords: Primulaceae ; tissue culture ; regeneration ; somaclonal variation ; organogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In long-term callus cultures of Cyclamen persicum Mill. two types of tissue could be distinguished. One type featured a brown suberised outer layer and was poorly organogenic. The other type was yellowish in appearance and gave rise to many shoot buds. Both types co-existed on the same callus, the former prevailing. Selection for organogenic tissue resulted in cultures yielding approximately three times more petioles than random subcultures. Callus-derived shoots could be rooted and established in the greenhouse. The method allowed for the production of thousands of plants but the regenerants often showed deviant phenotypes and genotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232991
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Shoot regeneration in long-term callus cultures derived from mature flowering plants of Cyclamen persicum Mill. (1996)
    Springer
    Plant cell reports 15 (1996), S. 545-548 
    Details
    ISSN: 1432-203X
    Keywords: Abbreviations: BA, 6-benzylaminopurine; BMP, basal medium propagation; BMR, basal medium rooting; DAPI, 4', 6-diamino-2-phenylindole; KIBA, potassium salt of indole-3-butyric acid; kinetin, 6-furfurylaminopurine; MS, Murashige and Skoog; NAA, 1-naphthaleneacetic acid ; Key words:Primulaceae, tissue culture, regeneration, somaclonal variation, organogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary. In long-term callus cultures of Cyclamen persicum Mill. two types of tissue could be distinguished. One type featured a brown suberised outer layer and was poorly organogenic. The other type was yellowish in appearance and gave rise to many shoot buds. Both types co-existed on the same callus, the former prevailing. Selection for organogenic tissue resulted in cultures yielding approximately three times more petioles than random subcultures. Callus-derived shoots could be rooted and established in the greenhouse. The method allowed for the production of thousands of plants but the regenerants often showed deviant phenotypes and genotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050071
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    A simple technique to overcome vitrification inGypsophila paniculata L. (1989)
    Springer
    Plant cell, tissue and organ culture 19 (1989), S. 181-188 
    Details
    ISSN: 1573-5044
    Keywords: Gypsophila paniculata L. ; vitrification ; micropropagation ; evaporation ; lag phase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Node cultures ofGypsophila paniculata showed vitrified growth when cultured under standard conditions, resulting in poor survival of tissue culture derived plants. The deviant growth pattern appeared to be determined in the lag phase of the cultures. A limited evaporation period in this stage induced normal growth. Re-establishment of high relative humidity and poorer gas exchange thereafter did not lead to vitrified growth. Vitrification was also depressed when evaporation was continued during the whole culture period, but shorter plantlets resulted. A simple adaptation of the plastic lid that is commonly used in micropropagation, allowed controlled and contamination-free evaporation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043345
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    Paper (German National Licenses)
    Fulltext
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