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  • 1
    Electronic Resource
    Electronic Resource
    Identification and mapping of three flower colour loci of potato (S. tuberosum L.) by RFLP analysis (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 295-300 
    Details
    ISSN: 1432-2242
    Keywords: Solanum tuberosum ; Allelism ; RFLP map ; Anthocyanin markers ; Tester clones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The inheritance of flower colour in diploid potato (2 n = 2x = 24), was found to be controlled by three unlinked loci D, F and P. To determine the allelism with previously described loci and to dissect this oligogenic trait, a set of tester clones with well-defined genotypes was developed. By backcrossing the mapping population with these tester clones it was possible to obtain monogenic segregation ratios. These were required to detect linkage with RFLP loci and, despite distorted Mendelian ratios, the inheritance and mapping of the D, F and P loci could be unambiguously determined. Locus D, involved in the biosynthesis of red anthocyanins, was mapped on chromosome 2, while locus P, involved in the production of blue anthocyanins, was mapped on chromosome 11. Locus F, involved in the flower-specific expression of gene(s) accommodated by the D and P loci, was mapped on chromosome 10. The tester clones and the map position of the D, F and P loci may be of considerable value in simplifying the genetics of anthocyanin pigmentation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222091
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Physiological and genetic control of tuber formation (1999)
    Springer
    Potato research 42 (1999), S. 313-331 
    Details
    ISSN: 1871-4528
    Keywords: tuberization ; stolon formation ; hormones ; morphological changes ; gibberellins ; QTL mapping ; fingerpringting ; carbohydrate metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Tuber formation is a plalstic and complex, but well-orchestrated sequences of morphological. physiological and biochemical events. The physiological control mechanisms of this sequence of events may involve many hormones, but certainly gibberellic acids play a dominant role: they affecft most steps and are influenced by inducing or non-inducing conditions in a manner consistent with effects of these conditions on tuber induction. The genetic control is also complex. Numerous cDNA fragments have been isolated which are specifically expressed during tuberization. They can be used in anti-sense orientation in transgenic plants to thest their possible role in tuberization. In addition these cDNA fragments are used as genetic marker loci in QTL mapping studies and serve as candidate genes to exlain phenotypic variation. Profiles of the importance of QTLs over time may be combined with expression profiles of candidate genes. Such novel approaches offer unique opportunities for synergism between physiology, molecular biology and genetics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02357860
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Mapping of resistance to the potato cyst nematode Globodera rostochiensis from the wild potato species Solanum vernei (1996)
    Springer
    Molecular breeding 2 (1996), S. 51-60 
    Details
    ISSN: 1572-9788
    Keywords: BSA ; Globodera rostochiensis ; potato ; RFLP ; SCAR ; Solanum vernei
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A population of diploid potato (Solanum tuberosum) was used for the genetic analysis and mapping of a locus for resistance to the potato cyst nematode Globodera rostochiensis, introgressed from the wild potato species Solanum vernei. Resistance tests of 108 genotypes of a F1 population revealed the presence of a single locus with a dominant allele for resistance to G. rostochiensis pathotype Ro1. This locus, designated GroV1, was located on chromosome 5 with RFLP markers. Fine-mapping was performed with RAPD and SCAR markers. The GroV1 locus was found in the same region of the potato genome as the S. tuberosum ssp. andigena H1 nematode resistance locus. Both resistance loci could not excluded to be allelic. The identification of markers flanking the GroV1 locus offers a valuable strategy for marker-assisted selection for introgression of this nematode resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00171351
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  • 4
    Electronic Resource
    Electronic Resource
    The potential of AFLPs in biosystematics: A first application inSolanum taxonomy(Solanaceae) (1998)
    Springer
    Plant systematics and evolution 210 (1998), S. 87-103 
    Details
    ISSN: 1615-6110
    Keywords: Solanaceae ; Solanum ; AFLP markers ; DNA fingerprinting ; phylogeny ; polyploids ; potato ; tomato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using the AFLP technique highly informative DNA fingerprints were generated from 19 taxa ofSolanum sect.Petota (potatoes) and three taxa ofSolanum sect.Lycopersicum (tomatoes). Both phenetic and cladistic analyses were conducted from the individual genotypic level to the species level. An AFLP fingerprint, using a combination of suitable AFLP primers, generated 12 to 71 scorable fragments per genotype which was sufficient for taxonomic interpretation. The classifications based on the molecular markers were generally in agreement with current taxonomic opinions. Unexpectedly,S. microdontum was associated with ser.Megistacroloba rather than with ser.Tuberosa, andS. demissum (ser.Demissa) and species of ser.Acaulia appeared closely affiliated. AFLP is an efficient and reliable technique to generate biosystematic data and therefore a promising tool for evolutionary studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00984729
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  • 5
    Electronic Resource
    Electronic Resource
    The inheritance and chromosomal localization of AFLP markers in a non-inbred potato offspring (1995)
    Springer
    Molecular breeding 1 (1995), S. 397-410 
    Details
    ISSN: 1572-9788
    Keywords: AFLP marker ; allelism ; allogamous ; linkage map ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract AFLPTM is a new technique to generate large numbers of molecular markers for genetic mapping. The method involves the selective amplification of a limited number of DNA restriction fragments out of complex plant genomic DNA digests using PCR. With six primer combinations 264 segregating AFLP amplification products were identified in a diploid backcross population from non-inbred potato parents. The identity of an AFLP marker was specified by the primer combination of the amplification product and its size estimated in bases. The segregating AFLP amplification products were mapped by using a mapping population with 217 already known RFLP, isozyme and morphological trait loci. In general, the AFLP markers were randomly distributed over the genome, although a few clusters were observed. No indications were found that AFLP markers are present in other parts of the genome than those already covered by RFLP markers. Locus specificity of AFLP markers was demonstrated because equally sized amplification products segregating from both parental clones generally mapped to indistinguishable maternal and paternal map positions. Locus specificity of AFLP amplification products will allow to establish the chromosomal identity of linkage groups in future mapping studies. Since AFLP technology is a multi-locus detection system, it was not possible to identify the AFLP alleles which belong to a single AFLP locus. The consequences of a genetic analysis based on single alleles, rather than on loci with two or more alleles on mapping studies using progenies of non-inbred parents are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01248417
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    Paper (German National Licenses)
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