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  • 1
    Electronic Resource
    Electronic Resource
    Dynamics of a gravitational billiard with a hyperbolic lower boundary (1999)
    Woodbury, NY : American Institute of Physics (AIP)
    Chaos 9 (1999), S. 841-848 
    Details
    ISSN: 1089-7682
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Gravitational billiards provide a simple method for the illustration of the dynamics of Hamiltonian systems. Here we examine a new billiard system with two parameters, which exhibits, in two limiting cases, the behaviors of two previously studied one-parameter systems, namely the wedge and parabolic billiard. The billiard consists of a point mass moving in two dimensions under the influence of a constant gravitational field with a hyperbolic lower boundary. An iterative mapping between successive collisions with the lower boundary is derived analytically. The behavior of the system during transformation from the wedge to the parabola is investigated for a few specific cases. It is surprising that the nature of the transformation depends strongly on the parameter values. © 1999 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.166467
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  • 2
    Electronic Resource
    Electronic Resource
    063
Electroporation-Facilitated Gene Therapy for Wound Healing (2005)
    Oxford, UK; Malden, USA : Blackwell Publishing Ltd/Inc.
    Wound repair and regeneration 13 (2005), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Introduction:  Using peptide growth factors to improve upon natural wound healing provides promise, but topical application of growth factors has found limited clinical success. Gene therapy has been limited by low transfection efficiency. We are investigating the use of electroporation (EP)in vivo to enhance transfection efficiency and improve wound healing with DNA expression vectors for growth factors.Methods:  To assess plasmid transfection and wound healing, gWIZ luciferase vector and PCDNA3.1/KGF expression vector were used, respectively. Cutaneous wounds were produced via 8 mm-punch biopsy in Sprague Dawley rats. Healing was impaired by cecal ligation induced sepsis. We used NIH image analysis software and histologic assessment to assess wound closure.Results:  Plasmid Transfection: EP effectively increased expression of gWIZ luciferase vector 53-fold compared to vector without EP (p 〈 0.001). We demonstrated that transfection was localized to skin when transfected skin was reflected to reveal underlying muscle. We found that EP-assisted transfection lasted for 30 days, which is appropriate for treatment of wound healing.Wound Healing:  Using PCDNA3.1/KGF expression vector and EP wounds were 60% smaller on day 12 versus vector without EP (p 〈 0.009). We assessed quality of healing with a histologic scoring system grading quality of epithelial coverage, organization of scar and resolution of inflammation. KGF vector + EP score of 3.0 +/− 0.3 was better than that of 1.8 +/− 0.3 for treatment with vector alone (p 〈 0.05).Conclusions:  These results demonstrate the capacity of electroporation-facilitated transfection with DNA plasmid expression vector for growth factor to improve wound healing.Acknowledgments:  Funding from USAMRMC PRMRP DAMDI7-03-1-0029 and Maryland State Firefighters Fund
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2005.130215bk.x
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  • 3
    Electronic Resource
    Electronic Resource
    067
Electroporation-Enhanced Gene Therapy with KGF-1 Corrects Healing Impairment in Older Diabetic Mice (2005)
    Oxford, UK; Malden, USA : Blackwell Publishing Ltd/Inc.
    Wound repair and regeneration 13 (2005), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Introduction:  Diabetes is known to impair wound healing. We assessed the effect of aging on this impairment and further explored the possibility of repairing age-dependant impairment by replenishing levels of the growth factor KGF-1 using our system of electroporation enhanced gene therapy.Methods:  Female BKS.Cg-m+/+Leprdb/J mice (diabetic) were obtained from the Jackson Laboratory (Bar Harbor, ME). Mice at time of study were 7, 27, and 84 weeks, respectively. Two wounds were created using a 5 mm punch biopsy. Plasmid DNA encoding KGF-1 was injected at 40 μg / wound. Electroporation was carried out with six square wave pulses, at 1800 volts for 100 μs; with aninterval of 125 ms. Wounds were assessed planometrically over a 14-day period.Results:  We found that wound healing is severely impaired in older, but not young diabetic mice (7-week-old mice displayed a threefold smaller wound area than 84-week-old mice on day 9). Glucose levels were similar in old and young groups ruling out differences in intensity of the metabolic derangement to explain this disparity (old: 334 +/− 41 mg/dl vs. young: 290 +/− 33 mg/dl). Treatment with KGF-1 gene therapy increased wound closure in aged mice 5.9-fold as compared to untreated aged mice 5 days after wounding (treated: 3724 +/− 631 vs. untreated: 632 +/− 517, P = 0.002). Aged KGF-1 gene therapy-treated mice closed wounds 10-fold faster than treated young mice 5 days after wounding (old: 3724: +/− 631 vs. young 358 +/− 871, P = 0.004).Conclusions:  We conclude that progressive damage to the tissues capability to heal occurs in individuals with diabetes. Treatment with KGF-1 gene therapy is more effective in aged mice than young mice. With an increasing geriatric diabetic population, gene therapy to replenish growth factors may be worth exploring.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2005.130215bo.x
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  • 4
    Electronic Resource
    Electronic Resource
    Evaluation of Air-Borne Particulates in Atmospheric Pollution Studies (1958)
    s.l. : American Chemical Society
    Analytical chemistry 30 (1958), S. 1172-1180 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac60139a002
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  • 5
    Electronic Resource
    Electronic Resource
    Solution structure of the glycosylphosphatidylinositol membrane anchor glycan of Trypanosoma brucei variant surface glycoprotein (1989)
    s.l. : American Chemical Society
    Biochemistry 28 (1989), S. 2881-2887 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00433a020
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  • 6
    Electronic Resource
    Electronic Resource
    Temperature dependence of photoconductivity in buckminsterfullerene films (1992)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 60 (1992), S. 1735-1737 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We report the temperature dependence from 330 to 230 K of the photoconductivity measured in sublimed films of buckminsterfullerene, specifically, C60/70, up to 3 μm thick. In contrast to the temperature dependence of the dark conductivity, where reversible structure, associated with the onset of a phase transition to orientational ordering below 250 K, is observed, the photoconductivity shows an essentially monotonic decrease to lower temperatures. This provides additional evidence that the thermal generation rate increases as the C60/70 becomes orientationally ordered. It also suggests that the increased order below the transition temperature does not lead to significantly increased carrier mobilities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.107201
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  • 7
    Electronic Resource
    Electronic Resource
    Heteroepitaxial nucleation of diamond on Si(001) in hot filament chemical vapor deposition (1995)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 66 (1995), S. 1900-1902 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Heteroepitaxial nucleation of diamond on mirror-polished Si(001) substrates was achieved in a conventional hot filament reactor. The key to this achievement was the development of a bias-enhanced nucleation process step analogous to the procedure for heteroepitaxial nucleation in microwave plasma chemical vapor deposition. The nucleation and subsequent growth of diamond were characterized by means of scanning electron microscopy, scanning tunneling microscopy, and Raman spectroscopy. Well-developed diamond (001) crystal faces are observed even for very thin (∼0.3 μm) diamond films. High-resolution scanning electron microscopy investigations of the early stage of growth revealed oriented crystallites on the substrate surface with a nucleation density of 109 cm−2. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.113315
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  • 8
    Electronic Resource
    Electronic Resource
    Mitochondrial mutation detection using enhanced multiplex denaturing high-performance liquid chromatography (2005)
    Oxford, UK : Blackwell Science Ltd
    International journal of immunogenetics 32 (2005), S. 0 
    Details
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: In this study, we investigated the presence of mutations within the mitochondrial genome in 40 Caucasian subjects using an enhanced multiplex denaturing high-performance liquid chromatography (DHPLC) approach. The enhanced DHPLC approach has increased sensitivity and throughput, and reduced analysis time per individual sample compared to conventional methods. This technique involved amplifying the mitochondrial genome in 18 fragments ranging in size from 300 to 2000 bp using a novel proofreading polymerase (OptimaseTM, Transgenomic Inc., Omaha, NE) with a low misincorporation rate. Fourteen of these fragments underwent subsequent restriction digestion using a combination of five restriction enzymes to enable multiplex DHPLC analysis; the remaining four underwent conventional DHPLC. Using this complete mitochondrial genome-screening approach, we confirmed a number of previously reported mutations and additionally identified a large number of novel mutations using an enhanced DHPLC technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1744-313X.2005.00508.x
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  • 9
    Electronic Resource
    Electronic Resource
    A Knowledge-based Program for Pressure Sore Prevention (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 463 (1986), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1986.tb21571.x
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  • 10
    Electronic Resource
    Electronic Resource
    Complete analysis of uranium-niobium-zirconium alloy by x-ray absorption edge technique (1968)
    s.l. : American Chemical Society
    Analytical chemistry 40 (1968), S. 27-29 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac60257a035
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