ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Osteoblasts were perceived as pivotal cells, recognized as the cells that control both the formativeand the resorptive phases of the bone remodeling cycle. Osteoblasts were an essential requirement forosteoclastogenesis though expressing or secreating bioactive osteoclast-differentiation-regulatory proteins,osteoclast differentiation factor (ODF)was the most important factor among these, ODF participate nearly inevery step of differentiation and activation of osteoclasts. In addition, intercellular adhesion molecule-1(ICAM-1)and its receptors LFA-1 play a role in osteoclast development by affecting adhesion betweenstromal cells and osteoclast progenitors before the occurrence of ODF-ODF receptor signaling. However, itis not clear about the relationship between ODF, ICAM-1 expression of osteoblasts and differentiation stateof osteoblasts. So,the aim of this study was to investgate whether the expression of ODF, ICAM-1 dependedon the stage of osteoblastic differentiation from rat bone marrow mesenchymal stem cells(rBMSCs). Theviability of rBMSCs is reduced significantly by osteogenic inducement as differentiating into osteoblasts,ALPase activity of OS-treated rBMSCs was enhanced obviously within 9 days , declined subsequently andrecovered nearly the original level at day 14. Expression of ODF is enhanced with osteogenic differentiationguadully. whereas, expression of ICAM-1 is activated at OS-treated day 6, then keeping at a stable level.This study indicated that rBMSCs undergoing osteogenic inducement was an ideal model for studying thedifferentiation and maturation of osteoblasts. During the early stage of differentiation along osteoblasts fromstem cells to osteocytes, rBMSCs or Osteoprogenitor react somewhat differently from osteoblasts,suggesting the ability of osteoblasts to regulating differentiation and maturation of osteoclasts have beenimproved with osteogenic culture
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/56/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.361-363.1173.pdf
Permalink
