ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
It was well recognized that mechanical strain plays a crucial role in periodontal tissuesremodeling. The aim of this study was to investigate the effect of mechanical strain on osteoblasticprecursor cells in a collagen type I gel scaffold. Rat MSCs were isolated and cultured according tothe established method. Cells were induced with osteogenic medium, then seeded in a collagen typeI gel and mechanically stretched by application of cyclic biaxial strain 24h later. Strain cycle was setto 1 cycle/min (0.017Hz), and strain magnitude was set to 2%, 5%, 7% elongation. Cells werecollected in 0h, 3h, 6h, 9h, 12h, 24h and 48h respectively. ODF and ICAM-1 mRNA were analyzedby RT-PCR assay. The results shown that 2-7% elongation strain, either dynamic or static, inhibitedICAM-1and ODF expression of osteoblastic precursors, and the effects were relative tightly to strainmagnitude. The inhibition effects of dynamic strain loading group exceeded the corresponding staticstrain. This work suggested that appropriate mechanical strech may suppress differentiation ofosteoclasts through inhibiting expression of ICAM-1 and ODF. Application of mechanical stressmight have a beneficial effect on quantity of generated bone tissue and might be a important factorin tissue engineering of periodontal tissues
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/53/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.1181.pdf
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