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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Vigna vexillata is a wild cross-incompatible relative of cowpea. It is highly resistant to several diseases and pests plaguing cowpea. A linkage map was developed for V. vexillata comprising 120 markers, including 70 random amplified polymorphic DNAs, 47 amplified fragment length polymorphisms, one simple sequence repeat and two morphological traits namely, the cowpea mottle carmovirus resistance locus (CPMo V) and leaf shape (La), utilizing an F2 generation of the intra-specific cross Tvnu 1443’× Tvnu 73′. The genetic map comprised 14 linkage groups spanning 1564.1 cM of the genome. Thirty-nine quantitative trait loci (QTLs) associated with nine traits were detected on the linkage map, explaining between 15.62 and 66.58% of their phenotypic variation. Seven chromosomal intervals contained QTLs with effects on multiple traits.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 121 (2002), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Cowpea mottle carmovirus (CPMoV) causes grain yield losses of up to 75% in cowpea (Vigna unguiculata [L.] Walp.). There is no resistance to this virus among cultivated cowpea lines, but a high level of resistance exists in Vigna vexillata, a wild Vigna species. Fifty-four accessions of V. vexillata germplasm collection at IITA were tested for resistance to CPMoV. Seedlings were mechanically inoculated with the virus and susceptibility or resistance was assessed by visual scoring of disease symptoms and serological analysis using antigen-coated plate enzyme-linked immunosorbent assay (ACP-ELISA). All but three V. vexillata lines belonging to the variety angustifolia were resistant to CPMoV. Crosses were made between two resistant V. vexillata lines and the three susceptible lines. Segregation patterns observed in the F2 and the backcross populations of all the crosses showed that resistance to CPMoV in V. vexillata is controlled by a single dominant gene, and the level of resistance conferred by this gene in V. vexillata is very high.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-203X
    Keywords: Key wordsVigna unguiculata ; V. vexillata ; Cowpea ; Embryo rescue ; Mineral salts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Factors responsible for successful rescue of immature embryos of cowpea [Vigna unguiculata (L.) Walp.] and V. vexillata (L.) and for in vitro embryo development were studied. A new basal medium for embryo development in vitro was formulated on the basis of the mineral composition of embryos. Sucrose, fructose and glucose were compared as carbohydrate sources. The highest frequency of embryos developing into plants was obtained with sucrose. Adding casein hydrolysate to the medium increased plant recovery by 30%. Among the plant growth factors used, cytokinins, zeatin, 6-benzylaminopurine and kinetin were the most effective in promoting embryo maturation and development. A method that can routinely ensure high plant recovery from cultured immature cowpea embryos is proposed.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 86 (1993), S. 97-104 
    ISSN: 1432-2242
    Keywords: Vigna ; Numerical taxonomy ; RFLP ; Asiatic grams ; Cowpea ; Bambara groundnut
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The taxonomy of the genus Vigna has been primarily based on morphological attributes. We have used 27 genomic clones from soybean, common bean, mungbean and cowpea to examine restriction fragment length polymorphism (RFLP) among 44 accessions of different species belonging to four subgenera of the genus Vigna. One accession each of common bean (Phaseolus vulgaris) and soybean (Glycine max) was included in the study. Total DNA from the various genotypes was digested with one restriction enzyme (Eco RV). Results of a numerical taxonomic analysis showed a high level of genetic variation within the genus with a remarkably higher amount of variation associated with Vigna sp. from Africa relative to those from Asia. The distinctness of the Asiatic grams in subgenus Ceratotropis, cowpea in section Catiang, bambara groundnut (V. subterranean) and members of the subgenus Plectotropis was elucidated by this study. Members of the subgenus Plectotropis were closer in genome homology to those of subgenus Vigna section Catiang than to those of subgenus Ceratotropis. The relative positions of some genotypes to one another on the dendrogram and minimum spanning tree were discussed in regard to hybridisations aimed generating well-saturated genomic maps and interspecies transfer of desirable genes.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 74 (1987), S. 483-486 
    ISSN: 1432-2242
    Keywords: Abelmoschus esculentus ; Okra ; Wide hybridization ; Pollen viability ; Unreduced pollen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Crosses were made between members of the two West African okra types ‘Soudanien’ and ‘Guineen’. All crosses succeeded in both directions and the F1 plants which showed hybrid vigour for plant stature were partially sterile. Cytological observations of the F1 plants revealed abnormal meiosis which resulted in the production of microspores of variable sizes. The frequency of viable pollen (as indicated by acetocarmine staining) was low in the hybrids: 35.80% (U.I.92× U.I.313) and 39.41% (1bk-1×U.I.215). The number of seeds produced per fruit was low in the hybrids and only a few of these seeds are viable. The possibility of gene transfer between the two okra types was discussed.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-2242
    Keywords: Parallel maps ; Molecular markers ; Random amplified polymorphic DNA ; Restriction fragment length polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genome relationships between mungbean (Vigna tradiata) and cowpea (V. Unguiculata) based on the linkage arrangement of random genomic restriction fragment length polymorphism (RFLP) markers have been investigated. A common set of probes derived from cowpea, common bean (Phaseolus vulgaris), mungbean, and soybean (Glycine max) PstI genomic libraries were used to construct the genetic linkage maps. In both species, a single F2 population from a cross between an improved cultivar and a putative wild progenitor species was used to follow the segregation of the RFLP markers. Approximately 90% of the probes hybridized to both mungbean and cowpea DNA, indicating a high degree of similarity in the nucleotide sequences among these species. A higher level of polymorphism was detected in the mungbean population (75.7%) than in the cowpea population (41.2%). Loci exhibiting duplications, null phenotypes, and distorted segregation ratios were detected in both populations. Random genomic DNA RFLP loci account for about 89% of the currently mapped markers with a few cDNA and RAPD markers added. The current mungbean map is comprised of 171 loci/loci clusters distributed in 14 linkage groups spanning a total of 1570cM. On the other hand, 97 markers covered 684 cM and defined 10 linkage groups in the current cowpea map. The mungbean and cowpea genomes were compared on the basis of the copy number and linkage arrangement of 53 markers mapped in common between the two species. Results indicate that nucleotide sequences are conserved, but variation in copy number were detected and several rearrangements in linkage orders appeared to have occurred since the divergence of the two species. Entire linkage groups were not conserved, but several large linkage blocks were maintained in both genomes.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-5060
    Keywords: Okra ; character correlations ; path coefficient analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Genotypic, phenotypic and environmental correlation coefficients were calculated for fifteen characters during two growing seasons. Correlation coefficients varie between seasons. Edible pod weight, edible pod length, edible pod width, number of seeds per plant, weight of 100 seeds, length of mature pods and number of branches per plant showed significant genotypic correlation with pod yield per plant; only number of branches per plant, edible pod length and weight of 100 seeds were phenotypically correlated with pod yield. Environmental correlation coefficients were generally low but edible pod length, final plant height and edible pod weight showed significant environmental correlation with pod yield during the two seasons. The genotypic correlation coefficients of selected eight characters with pod yield were partitioned into direct and indirect causes. In the early seasons, edible pod weight had the largest positive direct effect on pod yield with its largest indirect effect through reduction in edible pod width. Edible pod width which was highly correlated with pod yield had a negative direct effect on pod yield. In the late season, edible pod weight had the largest direct effect on pod yield, with large indirect effects through reduction in number of days to flowering and number of pods per plant. Number of days to flowering had a large direct effect on pod yield with its largest indirect effect through reduction in edible pod weight. The residual factors during the two seasons were negative. The study indicated that only number of branches per plant, edible pod length and weight of 100 seeds would be useful for indirect selection for pod yield. The path analysis indicated that edible pod weight was the most reliable and effective character to select for when high yield is the objective.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 39 (1988), S. 195-198 
    ISSN: 1573-5060
    Keywords: Xanthosoma sagittifolium ; cocoyam ; pollen ; storage ; viability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of different storage conditions on cocoyam pollen viability was investigated. Two levels of temperature (0° and 5°C) and four levels of relative humidity (0, 10, 20 and 30 percent) represented the storage environments. Viable cocoyam pollen could be obtained after 28 days in storage. The best storage condition was 5°C and 30% relative humidity.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 54 (1991), S. 137-140 
    ISSN: 1573-5060
    Keywords: Vigna species ; Vigna unguiculata ; cowpea ; Vigna unguiculata subspecies dekindtiana var. dekindtiana ; Vigna vexillata ; hairy cowpea ; wide crosses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Attempts were made to cross a wild profusely hairy cowpea (Vigna vexillata) with other cowpea types (both cultivated and non-cultivated) for the purpose of transferring gene(s) for hairiness from the wild to the cultivated genepool. No hybrid was however obtained.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 91 (1996), S. 181-187 
    ISSN: 1573-5060
    Keywords: Aphis craccivora ; cowpea ; DNA markers ; insect ; legume ; restriction fragment length polymorphisms ; Vigna unguiculata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Restriction fragment length polymorphism (RFLP) analysis has several advantages over traditional methods of genetic linkage mapping, one of these being the starting point for map-based cloning. The recent development of an RFLP map of cowpea (Vigna unguiculata L. Walp) has allowed the investigation of associations between genes of interest and RFLP markers. A cross between an aphid (Aphis craccivora Koch) resistant cultivated cowpea, TT84S-2246-4, and an aphid susceptible wild cowpea, NI 963, was screened for both aphid phenotype and RFLP marker segregation. One RFLP marker, bg4D9b, was found to be tightly linked to the aphid resistance gene (Rac 1) and several flanking markers in the same linkage group (linkage group 1) were also identified. The close association of Rac 1 and RFLP bg4D9b presents a real potential for cloning this insect resistance gene.
    Type of Medium: Electronic Resource
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