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  • 1
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: 3-[123I]Iodo-L-α-methyl tyrosine (123I-IMT) is used for diagnosis and monitoring of brain tumours by means of single-photon emission tomography. As recently shown, 123I-IMT is predominantly mediated into rat C6 glioma cells by sodium-independent system L for large neutral amino acids. Until now, 123I-IMT transport in non-neoplastic glial cells has not been examined. Therefore, the aim of this study was to examine the cellular pathways and precise transport kinetics of 123I-IMT uptake into astrocytes of neonatal rats. In particular sodium-independent 123I-IMT transport into neonatal astrocytes was compared with sodium-independent 123I-IMT uptake into neoplastic rat C6 glioma cells.Competitive inhibition experiments showed that 123I-IMT is exclusively transported via sodium-independent system L into the neonatal astrocytes (92%). Kinetic analysis of sodium-independent 123I-IMT uptake into neonatal astrocytes and into C6 glioma cells revealed apparent Michaelis constants KM = 13.9 ± 0.5 µm and KM = 33.9 ± 4.1 µm, respectively, which are in the same range of KM values as those recently determined for amino acid transport into neoplastic and non-neoplastic glial cells. Indeed, the KM values in the micromolar range correspond to the expression of the LAT-1 subunit of system L both in the neonatal astrocytes and in C6 glioma cells. However, sodium-independent maximum transport velocities (Vmax) differed significantly between neonatal astrocytes and C6 glioma cells (11.1 ± 0.3 and 39.9 ± 3.3 nmol/mg protein/10 min, respectively).
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Oncostatin M (OSM) and other members of the interleukin-6 cytokines, like ciliary neurotrophic factor and leukemia inhibitory factor, can induce differentiation of glial cells. We have recently described that OSM inhibited the growth of human glioma cells in vitro and induced a cell morphology resembling that of mature astrocytes. Using the glioblastoma cell line 86HG39, we demonstrated that treatment of the glioma cells with OSM also leads to a differentiation of the malignant glioma cells as judged by a strong increase in glial fibrillary acidic protein expression. The differentiation and the growth inhibition were not significantly blocked by expression of a dominant-negative (dn) signal transducer and activator of transcription (Stat) 3 protein. OSM exerted a reduction in DNA synthesis even in the presence of a high expression level of dnStat3. Moreover, inhibition of the ras-raf-mitogen-activated protein kinase (MAPK) pathway by the MAPK kinase 1 inhibitor PD98059 resulted in a synergistic enhancement of the OSM effect, indicating that the activation of this pathway counteracts the activity of the cytokine.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We describe here the oncostatin M (OSM)-dependent inhibition of in vivo tumour formation after intracerebral inoculation of glioblastoma cells in mice. We generated human glioblastoma cells transfected with the OSM gene under the control of a tetracycline-response promoter. Upon removal of tetracycline from the medium, cells exhibited a differentiated cell morphology, while proliferation was significantly inhibited. After implantation of these cells into nude mice brains, large tumours developed in animals lacking OSM expression, whereas no tumour formation was observed in mice with induced OSM expression. Our results suggest that OSM exerts pronounced antitumorigenic effects on glioblastoma cells in vivo and provide arguments for a therapeutic application of OSM in humans.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Herz 25 (2000), S. 384-391 
    ISSN: 1615-6692
    Keywords: Key Words Cardiomyopathy ; Magnetic resonance imaging ; Schlüsselwörter Kardiomyopathie ; Magnetresonanztomographie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die kardiale Magnetresonanztomographie (MRT) bietet als nichtinvasives Verfahren die Möglichkeit, innerhalb einer Untersuchung die für die verschiedenen Formen der Kardiomyopathien charakteristischen funktionellen und morphologischen Veränderungen darzustellen. In dieser Übersicht werden die Einsatzmöglichkeiten der MRT bei den verschiedenen Formen dieser Erkrankung diskutiert. Die geringe Variabilität der MRT-Messungen gestattet ge-naue Verlaufskontrollen im klinischen Alltag, eröffnet aber auch die Möglichkeit, Therapiestudien mit einer geringeren Anzahl von einzuschließenden Patienten zu sicheren Ergebnissen zu führen. Damit erweitert sich das Spektrum der Indikationen über die Präzisierung unklarer Befunde hinaus auf die Verlaufsbeobachtung während einer pharmakologischen Intervention und auf alle wissenschaftlichen Fragestellungen. Der Vorzug der MRT, nichtinvasiv Gewebe charakterisieren zu können, verdeutlicht das Potential dieser Technik bei sekundären Kardiomyopathien sowie bei entzündlichen und infiltrativen Formen myokardialer Erkrankungen.
    Notes: Abstract Cardiac magnetic resonance imaging (MRI) is a noninvasive tool which is able to diagnose and differentiate cardiomyopathies in a single study. The assessment of essential information such as alterations of myocardial and ventricular geometry and function is possible with a high degree of accuracy and reproducibility, based on a small inter- and intraobserver variability. Thus, very small morphological and functional changes in different types of cardiomyopathy are detectable, thereby enabling the cardiologist to increase the safety of therapeutic decisions. Furthermore, MRI bears the potential to characterize tissue transformation in the different types of myocardial affections including ischemic, toxic, infiltrative or inflammatory forms.
    Type of Medium: Electronic Resource
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